Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21250 and BWI76_RS21255 overlap by 14 nucleotides BWI76_RS21255 and BWI76_RS21260 are separated by 13 nucleotides BWI76_RS21260 and BWI76_RS21265 are separated by 545 nucleotides
BWI76_RS21250: BWI76_RS21250 - two-component system response regulator GlrR, at 4,448,887 to 4,450,224
_RS21250
BWI76_RS21255: BWI76_RS21255 - hypothetical protein, at 4,450,211 to 4,450,915
_RS21255
BWI76_RS21260: BWI76_RS21260 - two-component sensor histidine kinase, at 4,450,929 to 4,452,383
_RS21260
BWI76_RS21265: BWI76_RS21265 - phosphoribosylformylglycinamidine synthase, at 4,452,929 to 4,456,816
_RS21265
Position (kb) 4450
4451
4452
4453 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4449.962 kb on - strand, within BWI76_RS21250 at 4449.962 kb on - strand, within BWI76_RS21250 at 4450.023 kb on + strand, within BWI76_RS21250 at 4450.055 kb on + strand, within BWI76_RS21250 at 4450.420 kb on - strand, within BWI76_RS21255 at 4450.468 kb on - strand, within BWI76_RS21255 at 4450.473 kb on + strand, within BWI76_RS21255 at 4450.481 kb on - strand at 4450.524 kb on - strand, within BWI76_RS21255 at 4450.689 kb on + strand, within BWI76_RS21255 at 4451.054 kb on + strand at 4451.054 kb on + strand at 4451.054 kb on + strand at 4451.062 kb on - strand at 4451.088 kb on - strand, within BWI76_RS21260 at 4451.483 kb on - strand, within BWI76_RS21260 at 4451.546 kb on + strand, within BWI76_RS21260 at 4451.694 kb on + strand, within BWI76_RS21260 at 4451.920 kb on - strand, within BWI76_RS21260 at 4451.953 kb on - strand, within BWI76_RS21260 at 4452.104 kb on + strand, within BWI76_RS21260 at 4452.111 kb on - strand, within BWI76_RS21260 at 4452.156 kb on - strand, within BWI76_RS21260 at 4452.251 kb on + strand at 4452.528 kb on + strand at 4452.940 kb on + strand at 4453.142 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 4,449,962 - BWI76_RS21250 0.80 +0.2 4,449,962 - BWI76_RS21250 0.80 -0.5 4,450,023 + BWI76_RS21250 0.85 -2.3 4,450,055 + BWI76_RS21250 0.87 -0.2 4,450,420 - BWI76_RS21255 0.30 +0.8 4,450,468 - BWI76_RS21255 0.36 -0.5 4,450,473 + BWI76_RS21255 0.37 +0.8 4,450,481 - +1.0 4,450,524 - BWI76_RS21255 0.44 -1.8 4,450,689 + BWI76_RS21255 0.68 -0.6 4,451,054 + -0.4 4,451,054 + +0.4 4,451,054 + +0.7 4,451,062 - +0.3 4,451,088 - BWI76_RS21260 0.11 -2.9 4,451,483 - BWI76_RS21260 0.38 -0.4 4,451,546 + BWI76_RS21260 0.42 +0.5 4,451,694 + BWI76_RS21260 0.53 -1.8 4,451,920 - BWI76_RS21260 0.68 -1.5 4,451,953 - BWI76_RS21260 0.70 -1.0 4,452,104 + BWI76_RS21260 0.81 -1.8 4,452,111 - BWI76_RS21260 0.81 -0.9 4,452,156 - BWI76_RS21260 0.84 -1.1 4,452,251 + -0.3 4,452,528 + -0.2 4,452,940 + +1.0 4,453,142 + +0.2
Or see this region's nucleotide sequence