Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14530 and BWI76_RS14535 are separated by 86 nucleotides BWI76_RS14535 and BWI76_RS14540 overlap by 4 nucleotides
BWI76_RS14530: BWI76_RS14530 - non-ribosomal peptide synthetase, at 3,036,018 to 3,042,116
_RS14530
BWI76_RS14535: BWI76_RS14535 - polyketide synthase, at 3,042,203 to 3,051,679
_RS14535
BWI76_RS14540: BWI76_RS14540 - yersiniabactin biosynthetic protein YbtU, at 3,051,676 to 3,052,773
_RS14540
Position (kb)
3042
3043
3044
3045
3046
3047
3048
3049
3050
3051
3052 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3041.484 kb on + strand, within BWI76_RS14530 at 3041.578 kb on - strand at 3041.591 kb on + strand at 3041.697 kb on - strand at 3041.716 kb on + strand at 3041.999 kb on + strand at 3042.461 kb on + strand at 3042.688 kb on + strand at 3042.688 kb on + strand at 3042.696 kb on - strand at 3042.785 kb on - strand at 3042.836 kb on - strand at 3042.962 kb on - strand at 3043.110 kb on - strand at 3043.244 kb on + strand, within BWI76_RS14535 at 3043.280 kb on - strand, within BWI76_RS14535 at 3043.319 kb on + strand, within BWI76_RS14535 at 3043.464 kb on - strand, within BWI76_RS14535 at 3043.536 kb on - strand, within BWI76_RS14535 at 3043.586 kb on + strand, within BWI76_RS14535 at 3043.858 kb on + strand, within BWI76_RS14535 at 3043.967 kb on - strand, within BWI76_RS14535 at 3044.088 kb on + strand, within BWI76_RS14535 at 3044.284 kb on + strand, within BWI76_RS14535 at 3044.367 kb on - strand, within BWI76_RS14535 at 3044.673 kb on + strand, within BWI76_RS14535 at 3044.681 kb on - strand, within BWI76_RS14535 at 3044.737 kb on + strand, within BWI76_RS14535 at 3045.029 kb on + strand, within BWI76_RS14535 at 3045.181 kb on + strand, within BWI76_RS14535 at 3045.307 kb on - strand, within BWI76_RS14535 at 3045.578 kb on + strand, within BWI76_RS14535 at 3045.771 kb on - strand, within BWI76_RS14535 at 3046.376 kb on - strand, within BWI76_RS14535 at 3046.468 kb on + strand, within BWI76_RS14535 at 3046.528 kb on + strand, within BWI76_RS14535 at 3046.536 kb on - strand, within BWI76_RS14535 at 3047.287 kb on + strand, within BWI76_RS14535 at 3047.317 kb on + strand, within BWI76_RS14535 at 3048.087 kb on - strand, within BWI76_RS14535 at 3048.335 kb on - strand, within BWI76_RS14535 at 3049.066 kb on + strand, within BWI76_RS14535 at 3049.241 kb on + strand, within BWI76_RS14535 at 3049.531 kb on - strand, within BWI76_RS14535 at 3049.672 kb on + strand, within BWI76_RS14535 at 3050.107 kb on + strand, within BWI76_RS14535 at 3050.115 kb on - strand, within BWI76_RS14535 at 3050.509 kb on + strand, within BWI76_RS14535 at 3050.710 kb on + strand, within BWI76_RS14535 at 3050.788 kb on + strand at 3050.838 kb on + strand at 3050.875 kb on - strand at 3051.608 kb on + strand at 3051.616 kb on - strand at 3051.749 kb on + strand at 3051.848 kb on + strand, within BWI76_RS14540 at 3052.055 kb on + strand, within BWI76_RS14540 at 3052.411 kb on + strand, within BWI76_RS14540
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 3,041,484 + BWI76_RS14530 0.90 -1.0 3,041,578 - -0.8 3,041,591 + +1.2 3,041,697 - +2.3 3,041,716 + +0.4 3,041,999 + +0.2 3,042,461 + +0.8 3,042,688 + +0.5 3,042,688 + +0.4 3,042,696 - +0.2 3,042,785 - +0.5 3,042,836 - +1.3 3,042,962 - +0.1 3,043,110 - +0.8 3,043,244 + BWI76_RS14535 0.11 -2.0 3,043,280 - BWI76_RS14535 0.11 +0.6 3,043,319 + BWI76_RS14535 0.12 -0.2 3,043,464 - BWI76_RS14535 0.13 +0.2 3,043,536 - BWI76_RS14535 0.14 +0.3 3,043,586 + BWI76_RS14535 0.15 -0.5 3,043,858 + BWI76_RS14535 0.17 +0.1 3,043,967 - BWI76_RS14535 0.19 +0.9 3,044,088 + BWI76_RS14535 0.20 +0.5 3,044,284 + BWI76_RS14535 0.22 +1.3 3,044,367 - BWI76_RS14535 0.23 -2.4 3,044,673 + BWI76_RS14535 0.26 +1.1 3,044,681 - BWI76_RS14535 0.26 -0.0 3,044,737 + BWI76_RS14535 0.27 +0.7 3,045,029 + BWI76_RS14535 0.30 -0.6 3,045,181 + BWI76_RS14535 0.31 +0.8 3,045,307 - BWI76_RS14535 0.33 +1.7 3,045,578 + BWI76_RS14535 0.36 +0.2 3,045,771 - BWI76_RS14535 0.38 +1.0 3,046,376 - BWI76_RS14535 0.44 +0.2 3,046,468 + BWI76_RS14535 0.45 +1.1 3,046,528 + BWI76_RS14535 0.46 +0.7 3,046,536 - BWI76_RS14535 0.46 +0.0 3,047,287 + BWI76_RS14535 0.54 -0.3 3,047,317 + BWI76_RS14535 0.54 +1.1 3,048,087 - BWI76_RS14535 0.62 -0.1 3,048,335 - BWI76_RS14535 0.65 -0.3 3,049,066 + BWI76_RS14535 0.72 +0.1 3,049,241 + BWI76_RS14535 0.74 +0.5 3,049,531 - BWI76_RS14535 0.77 +0.4 3,049,672 + BWI76_RS14535 0.79 +1.8 3,050,107 + BWI76_RS14535 0.83 +2.0 3,050,115 - BWI76_RS14535 0.83 +0.1 3,050,509 + BWI76_RS14535 0.88 +0.7 3,050,710 + BWI76_RS14535 0.90 -1.4 3,050,788 + -0.3 3,050,838 + +2.2 3,050,875 - -1.0 3,051,608 + +0.5 3,051,616 - +0.1 3,051,749 + +0.2 3,051,848 + BWI76_RS14540 0.16 +0.4 3,052,055 + BWI76_RS14540 0.35 +0.3 3,052,411 + BWI76_RS14540 0.67 +2.2
Or see this region's nucleotide sequence