Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12740 and BWI76_RS12745 are separated by 76 nucleotides BWI76_RS12745 and BWI76_RS12750 overlap by 11 nucleotides BWI76_RS12750 and BWI76_RS12755 are separated by 7 nucleotides BWI76_RS12755 and BWI76_RS12760 are separated by 229 nucleotides
BWI76_RS12740: BWI76_RS12740 - hypothetical protein, at 2,658,273 to 2,658,635
_RS12740
BWI76_RS12745: BWI76_RS12745 - putative prophage membrane protein, at 2,658,712 to 2,659,104
_RS12745
BWI76_RS12750: BWI76_RS12750 - hypothetical protein, at 2,659,094 to 2,659,366
_RS12750
BWI76_RS12755: BWI76_RS12755 - hypothetical protein, at 2,659,374 to 2,659,916
_RS12755
BWI76_RS12760: BWI76_RS12760 - hypothetical protein, at 2,660,146 to 2,661,324
_RS12760
Position (kb)
2659
2660 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2658.223 kb on - strand at 2658.237 kb on + strand at 2658.264 kb on - strand at 2658.264 kb on - strand at 2658.358 kb on + strand, within BWI76_RS12740 at 2658.361 kb on - strand, within BWI76_RS12740 at 2658.366 kb on - strand, within BWI76_RS12740 at 2658.366 kb on - strand, within BWI76_RS12740 at 2658.377 kb on + strand, within BWI76_RS12740 at 2658.385 kb on - strand, within BWI76_RS12740 at 2658.398 kb on + strand, within BWI76_RS12740 at 2658.398 kb on + strand, within BWI76_RS12740 at 2658.404 kb on + strand, within BWI76_RS12740 at 2658.406 kb on - strand, within BWI76_RS12740 at 2658.406 kb on - strand, within BWI76_RS12740 at 2658.406 kb on - strand at 2658.412 kb on - strand, within BWI76_RS12740 at 2658.412 kb on - strand, within BWI76_RS12740 at 2658.417 kb on + strand, within BWI76_RS12740 at 2658.419 kb on + strand, within BWI76_RS12740 at 2658.419 kb on + strand, within BWI76_RS12740 at 2658.422 kb on - strand, within BWI76_RS12740 at 2658.425 kb on - strand, within BWI76_RS12740 at 2658.427 kb on - strand, within BWI76_RS12740 at 2658.427 kb on - strand, within BWI76_RS12740 at 2658.430 kb on + strand, within BWI76_RS12740 at 2658.430 kb on + strand, within BWI76_RS12740 at 2658.430 kb on + strand, within BWI76_RS12740 at 2658.430 kb on + strand, within BWI76_RS12740 at 2658.436 kb on - strand, within BWI76_RS12740 at 2658.438 kb on - strand, within BWI76_RS12740 at 2658.438 kb on - strand, within BWI76_RS12740 at 2658.438 kb on - strand, within BWI76_RS12740 at 2658.438 kb on - strand, within BWI76_RS12740 at 2658.438 kb on - strand, within BWI76_RS12740 at 2658.478 kb on - strand, within BWI76_RS12740 at 2658.548 kb on + strand, within BWI76_RS12740 at 2658.556 kb on - strand, within BWI76_RS12740 at 2658.619 kb on + strand at 2658.631 kb on + strand at 2658.638 kb on + strand at 2658.646 kb on - strand at 2658.689 kb on - strand at 2658.696 kb on + strand at 2658.704 kb on - strand at 2658.708 kb on + strand at 2658.714 kb on - strand at 2658.714 kb on - strand at 2658.716 kb on - strand at 2658.918 kb on - strand, within BWI76_RS12745 at 2658.955 kb on + strand, within BWI76_RS12745 at 2658.963 kb on - strand, within BWI76_RS12745 at 2658.987 kb on - strand, within BWI76_RS12745 at 2658.994 kb on - strand, within BWI76_RS12745 at 2659.056 kb on + strand, within BWI76_RS12745 at 2659.117 kb on + strand at 2659.117 kb on + strand at 2659.117 kb on + strand at 2659.117 kb on + strand at 2659.122 kb on + strand, within BWI76_RS12750 at 2659.122 kb on + strand, within BWI76_RS12750 at 2659.125 kb on - strand, within BWI76_RS12750 at 2659.130 kb on - strand, within BWI76_RS12750 at 2659.130 kb on - strand, within BWI76_RS12750 at 2659.177 kb on + strand, within BWI76_RS12750 at 2659.325 kb on + strand, within BWI76_RS12750 at 2659.325 kb on + strand, within BWI76_RS12750 at 2659.327 kb on + strand, within BWI76_RS12750 at 2659.330 kb on + strand, within BWI76_RS12750 at 2659.440 kb on + strand, within BWI76_RS12755 at 2659.440 kb on + strand, within BWI76_RS12755 at 2659.440 kb on + strand, within BWI76_RS12755 at 2659.487 kb on + strand, within BWI76_RS12755 at 2659.487 kb on + strand, within BWI76_RS12755 at 2659.495 kb on - strand, within BWI76_RS12755 at 2659.544 kb on + strand, within BWI76_RS12755 at 2659.552 kb on - strand, within BWI76_RS12755 at 2659.647 kb on + strand at 2659.736 kb on - strand, within BWI76_RS12755 at 2659.739 kb on + strand, within BWI76_RS12755 at 2659.881 kb on + strand at 2659.881 kb on + strand at 2659.986 kb on + strand at 2659.987 kb on + strand at 2659.996 kb on + strand at 2659.996 kb on + strand at 2660.063 kb on + strand at 2660.063 kb on + strand at 2660.226 kb on + strand at 2660.231 kb on + strand at 2660.231 kb on + strand at 2660.239 kb on - strand at 2660.302 kb on + strand, within BWI76_RS12760 at 2660.316 kb on - strand, within BWI76_RS12760 at 2660.316 kb on - strand, within BWI76_RS12760 at 2660.332 kb on + strand, within BWI76_RS12760 at 2660.341 kb on + strand, within BWI76_RS12760 at 2660.341 kb on + strand, within BWI76_RS12760 at 2660.341 kb on + strand, within BWI76_RS12760 at 2660.343 kb on + strand, within BWI76_RS12760 at 2660.348 kb on + strand, within BWI76_RS12760 at 2660.348 kb on + strand, within BWI76_RS12760
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 2,658,223 - -2.3 2,658,237 + -0.4 2,658,264 - -0.1 2,658,264 - +0.5 2,658,358 + BWI76_RS12740 0.23 -1.8 2,658,361 - BWI76_RS12740 0.24 -1.9 2,658,366 - BWI76_RS12740 0.26 +0.6 2,658,366 - BWI76_RS12740 0.26 -0.8 2,658,377 + BWI76_RS12740 0.29 -3.0 2,658,385 - BWI76_RS12740 0.31 +0.7 2,658,398 + BWI76_RS12740 0.34 -1.9 2,658,398 + BWI76_RS12740 0.34 +0.5 2,658,404 + BWI76_RS12740 0.36 +0.4 2,658,406 - BWI76_RS12740 0.37 -0.5 2,658,406 - BWI76_RS12740 0.37 -1.5 2,658,406 - +2.1 2,658,412 - BWI76_RS12740 0.38 +0.7 2,658,412 - BWI76_RS12740 0.38 -2.9 2,658,417 + BWI76_RS12740 0.40 -1.9 2,658,419 + BWI76_RS12740 0.40 +1.6 2,658,419 + BWI76_RS12740 0.40 -0.9 2,658,422 - BWI76_RS12740 0.41 +0.1 2,658,425 - BWI76_RS12740 0.42 -1.3 2,658,427 - BWI76_RS12740 0.42 +0.1 2,658,427 - BWI76_RS12740 0.42 -1.4 2,658,430 + BWI76_RS12740 0.43 -2.9 2,658,430 + BWI76_RS12740 0.43 +1.1 2,658,430 + BWI76_RS12740 0.43 +2.5 2,658,430 + BWI76_RS12740 0.43 -1.5 2,658,436 - BWI76_RS12740 0.45 -0.3 2,658,438 - BWI76_RS12740 0.45 +0.5 2,658,438 - BWI76_RS12740 0.45 -0.7 2,658,438 - BWI76_RS12740 0.45 +0.4 2,658,438 - BWI76_RS12740 0.45 -1.3 2,658,438 - BWI76_RS12740 0.45 -2.5 2,658,478 - BWI76_RS12740 0.56 -0.9 2,658,548 + BWI76_RS12740 0.76 +1.9 2,658,556 - BWI76_RS12740 0.78 -1.2 2,658,619 + -0.9 2,658,631 + -0.8 2,658,638 + +0.1 2,658,646 - +1.1 2,658,689 - -1.0 2,658,696 + +1.0 2,658,704 - +0.6 2,658,708 + -1.8 2,658,714 - -1.3 2,658,714 - -0.8 2,658,716 - +0.0 2,658,918 - BWI76_RS12745 0.52 +0.3 2,658,955 + BWI76_RS12745 0.62 +0.5 2,658,963 - BWI76_RS12745 0.64 -3.0 2,658,987 - BWI76_RS12745 0.70 -0.3 2,658,994 - BWI76_RS12745 0.72 -0.8 2,659,056 + BWI76_RS12745 0.88 +1.0 2,659,117 + -1.8 2,659,117 + +0.0 2,659,117 + +1.0 2,659,117 + -0.7 2,659,122 + BWI76_RS12750 0.10 -2.4 2,659,122 + BWI76_RS12750 0.10 +1.0 2,659,125 - BWI76_RS12750 0.11 -0.6 2,659,130 - BWI76_RS12750 0.13 -0.5 2,659,130 - BWI76_RS12750 0.13 +1.0 2,659,177 + BWI76_RS12750 0.30 +1.0 2,659,325 + BWI76_RS12750 0.85 -1.0 2,659,325 + BWI76_RS12750 0.85 -2.7 2,659,327 + BWI76_RS12750 0.85 +0.6 2,659,330 + BWI76_RS12750 0.86 +0.0 2,659,440 + BWI76_RS12755 0.12 -0.5 2,659,440 + BWI76_RS12755 0.12 +1.0 2,659,440 + BWI76_RS12755 0.12 -2.0 2,659,487 + BWI76_RS12755 0.21 -1.9 2,659,487 + BWI76_RS12755 0.21 +1.3 2,659,495 - BWI76_RS12755 0.22 +2.0 2,659,544 + BWI76_RS12755 0.31 +2.6 2,659,552 - BWI76_RS12755 0.33 +0.2 2,659,647 + -0.2 2,659,736 - BWI76_RS12755 0.67 +1.5 2,659,739 + BWI76_RS12755 0.67 +0.7 2,659,881 + +3.0 2,659,881 + -0.1 2,659,986 + -2.3 2,659,987 + -0.5 2,659,996 + +1.0 2,659,996 + +1.0 2,660,063 + -1.0 2,660,063 + +1.6 2,660,226 + +1.0 2,660,231 + +1.5 2,660,231 + -0.7 2,660,239 - -0.3 2,660,302 + BWI76_RS12760 0.13 -1.0 2,660,316 - BWI76_RS12760 0.14 +2.6 2,660,316 - BWI76_RS12760 0.14 +0.3 2,660,332 + BWI76_RS12760 0.16 +0.0 2,660,341 + BWI76_RS12760 0.17 -1.0 2,660,341 + BWI76_RS12760 0.17 +0.8 2,660,341 + BWI76_RS12760 0.17 -2.1 2,660,343 + BWI76_RS12760 0.17 -1.1 2,660,348 + BWI76_RS12760 0.17 +1.0 2,660,348 + BWI76_RS12760 0.17 -1.3
Or see this region's nucleotide sequence