Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12530 and BWI76_RS12535 are separated by 412 nucleotides BWI76_RS12535 and BWI76_RS12540 are separated by 262 nucleotides BWI76_RS12540 and BWI76_RS12545 are separated by 63 nucleotides
BWI76_RS12530: BWI76_RS12530 - sugar (glycoside-Pentoside-Hexuronide) transporter, at 2,617,234 to 2,618,688
_RS12530
BWI76_RS12535: BWI76_RS12535 - hypothetical protein, at 2,619,101 to 2,619,304
_RS12535
BWI76_RS12540: BWI76_RS12540 - maltoporin, at 2,619,567 to 2,620,943
_RS12540
BWI76_RS12545: BWI76_RS12545 - hypothetical protein, at 2,621,007 to 2,621,966
_RS12545
Position (kb)
2619
2620
2621 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2618.687 kb on + strand at 2618.695 kb on - strand at 2618.706 kb on + strand at 2618.709 kb on - strand at 2618.709 kb on - strand at 2618.711 kb on - strand at 2618.714 kb on - strand at 2618.714 kb on - strand at 2618.714 kb on - strand at 2618.826 kb on - strand at 2618.869 kb on - strand at 2618.869 kb on - strand at 2618.869 kb on - strand at 2618.998 kb on + strand at 2618.998 kb on + strand at 2618.998 kb on + strand at 2619.006 kb on - strand at 2619.056 kb on + strand at 2619.064 kb on - strand at 2619.118 kb on + strand at 2619.118 kb on + strand at 2619.118 kb on + strand at 2619.118 kb on + strand at 2619.118 kb on + strand at 2619.118 kb on + strand at 2619.118 kb on + strand at 2619.118 kb on + strand at 2619.118 kb on + strand at 2619.118 kb on + strand at 2619.118 kb on + strand at 2619.118 kb on + strand at 2619.120 kb on + strand at 2619.120 kb on + strand at 2619.123 kb on + strand, within BWI76_RS12535 at 2619.123 kb on + strand, within BWI76_RS12535 at 2619.123 kb on + strand, within BWI76_RS12535 at 2619.126 kb on - strand, within BWI76_RS12535 at 2619.128 kb on + strand, within BWI76_RS12535 at 2619.168 kb on + strand, within BWI76_RS12535 at 2619.168 kb on + strand, within BWI76_RS12535 at 2619.181 kb on + strand, within BWI76_RS12535 at 2619.235 kb on + strand, within BWI76_RS12535 at 2619.235 kb on + strand, within BWI76_RS12535 at 2619.265 kb on + strand, within BWI76_RS12535 at 2619.267 kb on - strand, within BWI76_RS12535 at 2619.267 kb on - strand, within BWI76_RS12535 at 2619.279 kb on + strand, within BWI76_RS12535 at 2619.279 kb on + strand, within BWI76_RS12535 at 2619.279 kb on + strand, within BWI76_RS12535 at 2619.282 kb on - strand at 2619.436 kb on - strand at 2619.450 kb on - strand at 2619.497 kb on + strand at 2619.622 kb on + strand at 2619.623 kb on + strand at 2619.631 kb on + strand at 2619.633 kb on + strand at 2619.633 kb on + strand at 2619.633 kb on + strand at 2619.633 kb on + strand at 2619.633 kb on + strand at 2619.633 kb on + strand at 2619.633 kb on + strand at 2619.633 kb on + strand at 2619.633 kb on + strand at 2619.633 kb on + strand at 2619.634 kb on + strand at 2619.641 kb on - strand at 2619.641 kb on - strand at 2619.641 kb on - strand at 2619.641 kb on - strand at 2619.654 kb on - strand at 2619.679 kb on - strand at 2619.679 kb on - strand at 2620.552 kb on + strand, within BWI76_RS12540 at 2620.552 kb on + strand, within BWI76_RS12540 at 2621.035 kb on - strand at 2621.048 kb on + strand at 2621.120 kb on + strand, within BWI76_RS12545 at 2621.475 kb on - strand, within BWI76_RS12545 at 2621.475 kb on - strand, within BWI76_RS12545
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 2,618,687 + -0.5 2,618,695 - +1.1 2,618,706 + -2.1 2,618,709 - +0.4 2,618,709 - +0.5 2,618,711 - +1.1 2,618,714 - +2.1 2,618,714 - -2.1 2,618,714 - +0.1 2,618,826 - +1.1 2,618,869 - +0.8 2,618,869 - +0.6 2,618,869 - +1.1 2,618,998 + +2.1 2,618,998 + -1.2 2,618,998 + -0.9 2,619,006 - -0.4 2,619,056 + +0.7 2,619,064 - +1.1 2,619,118 + +1.6 2,619,118 + -0.3 2,619,118 + +1.5 2,619,118 + -0.5 2,619,118 + -2.5 2,619,118 + +1.5 2,619,118 + -1.0 2,619,118 + +0.7 2,619,118 + -0.9 2,619,118 + +1.9 2,619,118 + -2.8 2,619,118 + +1.1 2,619,120 + +1.7 2,619,120 + -0.2 2,619,123 + BWI76_RS12535 0.11 -0.3 2,619,123 + BWI76_RS12535 0.11 -2.2 2,619,123 + BWI76_RS12535 0.11 +0.3 2,619,126 - BWI76_RS12535 0.12 -2.7 2,619,128 + BWI76_RS12535 0.13 -0.4 2,619,168 + BWI76_RS12535 0.33 -0.2 2,619,168 + BWI76_RS12535 0.33 -3.0 2,619,181 + BWI76_RS12535 0.39 +0.2 2,619,235 + BWI76_RS12535 0.66 -1.2 2,619,235 + BWI76_RS12535 0.66 -0.1 2,619,265 + BWI76_RS12535 0.80 +0.8 2,619,267 - BWI76_RS12535 0.81 -1.9 2,619,267 - BWI76_RS12535 0.81 -2.5 2,619,279 + BWI76_RS12535 0.87 +0.1 2,619,279 + BWI76_RS12535 0.87 +0.1 2,619,279 + BWI76_RS12535 0.87 +2.1 2,619,282 - +1.1 2,619,436 - +1.5 2,619,450 - +0.8 2,619,497 + +1.1 2,619,622 + -0.8 2,619,623 + -0.3 2,619,631 + +0.4 2,619,633 + -2.8 2,619,633 + +0.1 2,619,633 + -0.0 2,619,633 + +1.6 2,619,633 + -1.7 2,619,633 + -2.2 2,619,633 + +0.8 2,619,633 + -0.5 2,619,633 + -2.2 2,619,633 + +0.0 2,619,634 + +0.7 2,619,641 - +0.1 2,619,641 - +0.1 2,619,641 - +0.2 2,619,641 - -1.2 2,619,654 - -0.5 2,619,679 - +0.6 2,619,679 - +2.4 2,620,552 + BWI76_RS12540 0.72 +0.7 2,620,552 + BWI76_RS12540 0.72 -0.7 2,621,035 - +0.3 2,621,048 + -0.2 2,621,120 + BWI76_RS12545 0.12 +0.6 2,621,475 - BWI76_RS12545 0.49 +0.8 2,621,475 - BWI76_RS12545 0.49 +0.4
Or see this region's nucleotide sequence