Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12270 and BWI76_RS12275 are separated by 38 nucleotides BWI76_RS12275 and BWI76_RS12280 are separated by 3 nucleotides BWI76_RS12280 and BWI76_RS12285 are separated by 1 nucleotides BWI76_RS12285 and BWI76_RS12290 are separated by 1 nucleotides BWI76_RS12290 and BWI76_RS12295 overlap by 4 nucleotides
BWI76_RS12270: BWI76_RS12270 - hypothetical protein, at 2,564,473 to 2,565,609
_RS12270
BWI76_RS12275: BWI76_RS12275 - hypothetical protein, at 2,565,648 to 2,565,857
_RS12275
BWI76_RS12280: BWI76_RS12280 - hypothetical protein, at 2,565,861 to 2,566,271
_RS12280
BWI76_RS12285: BWI76_RS12285 - hypothetical protein, at 2,566,273 to 2,566,656
_RS12285
BWI76_RS12290: BWI76_RS12290 - hypothetical protein, at 2,566,658 to 2,567,209
_RS12290
BWI76_RS12295: BWI76_RS12295 - hypothetical protein, at 2,567,206 to 2,567,598
_RS12295
Position (kb)
2565
2566
2567 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2564.889 kb on + strand at 2564.902 kb on + strand, within BWI76_RS12270 at 2565.276 kb on + strand, within BWI76_RS12270 at 2565.387 kb on - strand, within BWI76_RS12270 at 2565.555 kb on + strand at 2565.724 kb on + strand, within BWI76_RS12275 at 2565.732 kb on - strand, within BWI76_RS12275 at 2565.801 kb on - strand, within BWI76_RS12275 at 2565.805 kb on + strand, within BWI76_RS12275 at 2565.813 kb on - strand, within BWI76_RS12275 at 2565.816 kb on - strand, within BWI76_RS12275 at 2565.917 kb on - strand, within BWI76_RS12280 at 2565.970 kb on + strand, within BWI76_RS12280 at 2565.978 kb on - strand, within BWI76_RS12280 at 2565.992 kb on + strand, within BWI76_RS12280 at 2566.222 kb on - strand, within BWI76_RS12280 at 2566.356 kb on + strand, within BWI76_RS12285 at 2566.435 kb on + strand, within BWI76_RS12285 at 2566.641 kb on + strand at 2566.713 kb on + strand at 2566.768 kb on + strand, within BWI76_RS12290 at 2566.810 kb on - strand, within BWI76_RS12290 at 2566.941 kb on + strand, within BWI76_RS12290 at 2566.943 kb on + strand, within BWI76_RS12290 at 2567.027 kb on - strand, within BWI76_RS12290 at 2567.027 kb on - strand, within BWI76_RS12290 at 2567.121 kb on - strand, within BWI76_RS12290 at 2567.164 kb on + strand at 2567.198 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 2,564,889 + +1.6 2,564,902 + BWI76_RS12270 0.38 +0.3 2,565,276 + BWI76_RS12270 0.71 -3.8 2,565,387 - BWI76_RS12270 0.80 -1.4 2,565,555 + -0.4 2,565,724 + BWI76_RS12275 0.36 +0.1 2,565,732 - BWI76_RS12275 0.40 -0.3 2,565,801 - BWI76_RS12275 0.73 -0.5 2,565,805 + BWI76_RS12275 0.75 -0.8 2,565,813 - BWI76_RS12275 0.79 -0.4 2,565,816 - BWI76_RS12275 0.80 -0.2 2,565,917 - BWI76_RS12280 0.14 -0.0 2,565,970 + BWI76_RS12280 0.27 -1.2 2,565,978 - BWI76_RS12280 0.28 -1.3 2,565,992 + BWI76_RS12280 0.32 -1.6 2,566,222 - BWI76_RS12280 0.88 -2.1 2,566,356 + BWI76_RS12285 0.22 -0.1 2,566,435 + BWI76_RS12285 0.42 -0.3 2,566,641 + +0.3 2,566,713 + -0.4 2,566,768 + BWI76_RS12290 0.20 -1.7 2,566,810 - BWI76_RS12290 0.28 -0.2 2,566,941 + BWI76_RS12290 0.51 +0.2 2,566,943 + BWI76_RS12290 0.52 -0.9 2,567,027 - BWI76_RS12290 0.67 -0.5 2,567,027 - BWI76_RS12290 0.67 +0.1 2,567,121 - BWI76_RS12290 0.84 +1.1 2,567,164 + +0.3 2,567,198 + +1.1
Or see this region's nucleotide sequence