Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02330 and BWI76_RS02335 are separated by 25 nucleotides BWI76_RS02335 and BWI76_RS02340 are separated by 154 nucleotides BWI76_RS02340 and BWI76_RS02345 are separated by 15 nucleotides
BWI76_RS02330: BWI76_RS02330 - aspartate ammonia-lyase, at 479,414 to 480,850
_RS02330
BWI76_RS02335: BWI76_RS02335 - hypothetical protein, at 480,876 to 481,034
_RS02335
BWI76_RS02340: BWI76_RS02340 - membrane protein FxsA, at 481,189 to 481,665
_RS02340
BWI76_RS02345: BWI76_RS02345 - L-methionine/branched-chain amino acid transporter, at 481,681 to 482,940
_RS02345
Position (kb) 480
481
482 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 479.889 kb on - strand, within BWI76_RS02330 at 479.970 kb on + strand, within BWI76_RS02330 at 480.014 kb on - strand, within BWI76_RS02330 at 480.112 kb on + strand, within BWI76_RS02330 at 480.120 kb on - strand, within BWI76_RS02330 at 480.131 kb on + strand, within BWI76_RS02330 at 480.131 kb on + strand, within BWI76_RS02330 at 480.139 kb on - strand, within BWI76_RS02330 at 480.325 kb on + strand, within BWI76_RS02330 at 480.370 kb on + strand, within BWI76_RS02330 at 480.436 kb on - strand, within BWI76_RS02330 at 480.471 kb on + strand, within BWI76_RS02330 at 480.498 kb on - strand, within BWI76_RS02330 at 480.498 kb on - strand, within BWI76_RS02330 at 480.537 kb on + strand, within BWI76_RS02330 at 480.564 kb on - strand, within BWI76_RS02330 at 480.567 kb on + strand, within BWI76_RS02330 at 480.604 kb on - strand, within BWI76_RS02330 at 480.689 kb on - strand, within BWI76_RS02330 at 480.689 kb on - strand, within BWI76_RS02330 at 480.752 kb on + strand at 480.799 kb on + strand at 480.870 kb on - strand at 480.947 kb on + strand, within BWI76_RS02335 at 480.959 kb on + strand, within BWI76_RS02335 at 481.137 kb on + strand at 481.172 kb on + strand at 481.258 kb on + strand, within BWI76_RS02340 at 481.259 kb on + strand, within BWI76_RS02340 at 481.259 kb on + strand, within BWI76_RS02340 at 481.259 kb on + strand, within BWI76_RS02340 at 481.267 kb on - strand, within BWI76_RS02340 at 481.279 kb on - strand, within BWI76_RS02340 at 481.317 kb on - strand, within BWI76_RS02340 at 481.365 kb on + strand, within BWI76_RS02340 at 481.611 kb on + strand, within BWI76_RS02340 at 481.694 kb on + strand at 481.737 kb on + strand at 481.818 kb on - strand, within BWI76_RS02345 at 481.867 kb on + strand, within BWI76_RS02345 at 481.967 kb on - strand, within BWI76_RS02345 at 481.969 kb on + strand, within BWI76_RS02345 at 481.977 kb on - strand, within BWI76_RS02345 at 481.977 kb on - strand, within BWI76_RS02345
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 479,889 - BWI76_RS02330 0.33 +0.7 479,970 + BWI76_RS02330 0.39 +0.8 480,014 - BWI76_RS02330 0.42 +0.4 480,112 + BWI76_RS02330 0.49 -0.5 480,120 - BWI76_RS02330 0.49 -0.5 480,131 + BWI76_RS02330 0.50 +0.2 480,131 + BWI76_RS02330 0.50 +0.5 480,139 - BWI76_RS02330 0.50 +0.4 480,325 + BWI76_RS02330 0.63 -0.9 480,370 + BWI76_RS02330 0.67 +0.0 480,436 - BWI76_RS02330 0.71 +1.0 480,471 + BWI76_RS02330 0.74 -1.3 480,498 - BWI76_RS02330 0.75 +0.3 480,498 - BWI76_RS02330 0.75 -0.0 480,537 + BWI76_RS02330 0.78 -0.2 480,564 - BWI76_RS02330 0.80 -2.1 480,567 + BWI76_RS02330 0.80 +0.9 480,604 - BWI76_RS02330 0.83 -0.5 480,689 - BWI76_RS02330 0.89 +0.0 480,689 - BWI76_RS02330 0.89 +0.4 480,752 + +1.5 480,799 + +0.7 480,870 - -0.6 480,947 + BWI76_RS02335 0.45 +0.6 480,959 + BWI76_RS02335 0.52 +0.7 481,137 + -0.2 481,172 + -0.4 481,258 + BWI76_RS02340 0.14 -1.5 481,259 + BWI76_RS02340 0.15 +0.5 481,259 + BWI76_RS02340 0.15 +1.0 481,259 + BWI76_RS02340 0.15 -1.3 481,267 - BWI76_RS02340 0.16 -2.8 481,279 - BWI76_RS02340 0.19 -1.8 481,317 - BWI76_RS02340 0.27 -4.2 481,365 + BWI76_RS02340 0.37 -1.7 481,611 + BWI76_RS02340 0.88 +0.4 481,694 + +0.1 481,737 + -0.3 481,818 - BWI76_RS02345 0.11 +0.6 481,867 + BWI76_RS02345 0.15 +1.0 481,967 - BWI76_RS02345 0.23 +1.3 481,969 + BWI76_RS02345 0.23 -0.7 481,977 - BWI76_RS02345 0.23 -0.2 481,977 - BWI76_RS02345 0.23 +0.5
Or see this region's nucleotide sequence