Experiment: Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12025 and BWI76_RS12030 overlap by 104 nucleotides BWI76_RS12030 and BWI76_RS12035 are separated by 9 nucleotides BWI76_RS12035 and BWI76_RS12040 are separated by 7 nucleotides BWI76_RS12040 and BWI76_RS12045 overlap by 4 nucleotides BWI76_RS12045 and BWI76_RS12050 overlap by 8 nucleotides BWI76_RS12050 and BWI76_RS12055 are separated by 34 nucleotides
BWI76_RS12025: BWI76_RS12025 - integrase, at 2,529,969 to 2,531,198
_RS12025
BWI76_RS12030: BWI76_RS12030 - hypothetical protein, at 2,531,095 to 2,531,409
_RS12030
BWI76_RS12035: BWI76_RS12035 - hypothetical protein, at 2,531,419 to 2,531,658
_RS12035
BWI76_RS12040: BWI76_RS12040 - hypothetical protein, at 2,531,666 to 2,531,974
_RS12040
BWI76_RS12045: BWI76_RS12045 - hypothetical protein, at 2,531,971 to 2,532,690
_RS12045
BWI76_RS12050: BWI76_RS12050 - hypothetical protein, at 2,532,683 to 2,533,036
_RS12050
BWI76_RS12055: BWI76_RS12055 - DNA single-strand annealing protein RecT-like protein, at 2,533,071 to 2,534,180
_RS12055
Position (kb)
2531
2532
2533 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2531.006 kb on - strand, within BWI76_RS12025 at 2531.085 kb on - strand at 2531.104 kb on + strand at 2531.143 kb on + strand at 2531.149 kb on + strand at 2531.151 kb on - strand at 2531.152 kb on - strand at 2531.226 kb on + strand, within BWI76_RS12030 at 2531.234 kb on - strand at 2531.313 kb on - strand, within BWI76_RS12030 at 2531.423 kb on - strand at 2531.461 kb on + strand, within BWI76_RS12035 at 2531.461 kb on + strand, within BWI76_RS12035 at 2531.461 kb on + strand, within BWI76_RS12035 at 2531.469 kb on - strand, within BWI76_RS12035 at 2531.469 kb on - strand, within BWI76_RS12035 at 2531.469 kb on - strand, within BWI76_RS12035 at 2531.708 kb on + strand, within BWI76_RS12040 at 2531.983 kb on + strand at 2532.114 kb on - strand, within BWI76_RS12045 at 2532.233 kb on + strand, within BWI76_RS12045 at 2532.627 kb on + strand at 2532.627 kb on + strand at 2532.642 kb on + strand at 2532.645 kb on - strand at 2532.869 kb on + strand, within BWI76_RS12050 at 2532.877 kb on - strand, within BWI76_RS12050 at 2532.937 kb on + strand, within BWI76_RS12050 at 2532.956 kb on - strand at 2532.989 kb on - strand, within BWI76_RS12050 at 2533.106 kb on - strand at 2533.276 kb on + strand, within BWI76_RS12055 at 2533.531 kb on - strand, within BWI76_RS12055 at 2533.662 kb on - strand, within BWI76_RS12055
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 2,531,006 - BWI76_RS12025 0.84 -1.9 2,531,085 - +1.5 2,531,104 + -2.8 2,531,143 + +0.5 2,531,149 + +0.1 2,531,151 - -0.2 2,531,152 - +0.0 2,531,226 + BWI76_RS12030 0.42 -1.4 2,531,234 - -1.2 2,531,313 - BWI76_RS12030 0.69 +1.6 2,531,423 - +1.1 2,531,461 + BWI76_RS12035 0.17 +0.8 2,531,461 + BWI76_RS12035 0.17 +0.1 2,531,461 + BWI76_RS12035 0.17 +0.1 2,531,469 - BWI76_RS12035 0.21 +1.0 2,531,469 - BWI76_RS12035 0.21 -0.6 2,531,469 - BWI76_RS12035 0.21 -1.3 2,531,708 + BWI76_RS12040 0.14 -1.5 2,531,983 + -1.6 2,532,114 - BWI76_RS12045 0.20 -1.4 2,532,233 + BWI76_RS12045 0.36 -0.8 2,532,627 + +1.7 2,532,627 + -1.7 2,532,642 + -0.1 2,532,645 - -0.3 2,532,869 + BWI76_RS12050 0.53 +1.7 2,532,877 - BWI76_RS12050 0.55 +0.8 2,532,937 + BWI76_RS12050 0.72 -1.8 2,532,956 - -1.4 2,532,989 - BWI76_RS12050 0.86 -1.3 2,533,106 - -1.4 2,533,276 + BWI76_RS12055 0.18 +0.6 2,533,531 - BWI76_RS12055 0.41 -0.8 2,533,662 - BWI76_RS12055 0.53 -0.7
Or see this region's nucleotide sequence