Experiment: Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10275 and BWI76_RS10280 overlap by 4 nucleotides BWI76_RS10280 and BWI76_RS10285 are separated by 28 nucleotides BWI76_RS10285 and BWI76_RS10290 are separated by 191 nucleotides
BWI76_RS10275: BWI76_RS10275 - ABC transporter, at 2,176,334 to 2,177,074
_RS10275
BWI76_RS10280: BWI76_RS10280 - polar amino acid ABC transporter permease, at 2,177,071 to 2,177,796
_RS10280
BWI76_RS10285: BWI76_RS10285 - family 3 extracellular solute-binding protein, at 2,177,825 to 2,178,637
_RS10285
BWI76_RS10290: BWI76_RS10290 - transcriptional regulator, at 2,178,829 to 2,179,725
_RS10290
Position (kb)
2177
2178
2179 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2176.990 kb on + strand, within BWI76_RS10275 at 2177.183 kb on + strand, within BWI76_RS10280 at 2177.191 kb on - strand, within BWI76_RS10280 at 2177.663 kb on + strand, within BWI76_RS10280 at 2177.696 kb on + strand, within BWI76_RS10280 at 2177.701 kb on + strand, within BWI76_RS10280 at 2177.706 kb on + strand, within BWI76_RS10280 at 2177.793 kb on + strand at 2177.981 kb on - strand, within BWI76_RS10285 at 2178.350 kb on - strand, within BWI76_RS10285 at 2178.471 kb on + strand, within BWI76_RS10285 at 2178.488 kb on - strand, within BWI76_RS10285 at 2178.842 kb on - strand at 2179.198 kb on + strand, within BWI76_RS10290 at 2179.198 kb on + strand, within BWI76_RS10290 at 2179.206 kb on - strand, within BWI76_RS10290 at 2179.206 kb on - strand, within BWI76_RS10290 at 2179.255 kb on + strand, within BWI76_RS10290 at 2179.499 kb on + strand, within BWI76_RS10290 at 2179.507 kb on - strand at 2179.520 kb on + strand at 2179.622 kb on + strand, within BWI76_RS10290
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 2,176,990 + BWI76_RS10275 0.89 +0.2 2,177,183 + BWI76_RS10280 0.15 +0.1 2,177,191 - BWI76_RS10280 0.17 -0.5 2,177,663 + BWI76_RS10280 0.82 -1.8 2,177,696 + BWI76_RS10280 0.86 +0.5 2,177,701 + BWI76_RS10280 0.87 -0.7 2,177,706 + BWI76_RS10280 0.87 -0.5 2,177,793 + -0.3 2,177,981 - BWI76_RS10285 0.19 -0.5 2,178,350 - BWI76_RS10285 0.65 +1.5 2,178,471 + BWI76_RS10285 0.79 -0.2 2,178,488 - BWI76_RS10285 0.82 -3.4 2,178,842 - -0.2 2,179,198 + BWI76_RS10290 0.41 +0.5 2,179,198 + BWI76_RS10290 0.41 +3.3 2,179,206 - BWI76_RS10290 0.42 +1.2 2,179,206 - BWI76_RS10290 0.42 +0.7 2,179,255 + BWI76_RS10290 0.47 -3.9 2,179,499 + BWI76_RS10290 0.75 +1.7 2,179,507 - +1.0 2,179,520 + -0.7 2,179,622 + BWI76_RS10290 0.88 +0.9
Or see this region's nucleotide sequence