Experiment: Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08715 and BWI76_RS08720 are separated by 230 nucleotides BWI76_RS08720 and BWI76_RS08725 overlap by 4 nucleotides
BWI76_RS08715: BWI76_RS08715 - acyl-CoA thioesterase, at 1,835,092 to 1,836,375
_RS08715
BWI76_RS08720: BWI76_RS08720 - imidazolonepropionase, at 1,836,606 to 1,837,823
_RS08720
BWI76_RS08725: BWI76_RS08725 - Formiminoglutamase (EC 3.5.3.8) (from data) , at 1,837,820 to 1,838,776
_RS08725
Position (kb)
1836
1837
1838 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1835.711 kb on - strand, within BWI76_RS08715 at 1835.788 kb on + strand, within BWI76_RS08715 at 1836.021 kb on + strand, within BWI76_RS08715 at 1836.029 kb on - strand, within BWI76_RS08715 at 1836.096 kb on + strand, within BWI76_RS08715 at 1836.330 kb on - strand at 1836.334 kb on + strand at 1836.434 kb on - strand at 1836.585 kb on + strand at 1836.688 kb on - strand at 1836.688 kb on - strand at 1837.299 kb on + strand, within BWI76_RS08720 at 1837.602 kb on + strand, within BWI76_RS08720 at 1837.653 kb on - strand, within BWI76_RS08720 at 1837.885 kb on - strand at 1838.181 kb on + strand, within BWI76_RS08725 at 1838.229 kb on + strand, within BWI76_RS08725 at 1838.484 kb on - strand, within BWI76_RS08725 at 1838.668 kb on + strand, within BWI76_RS08725 at 1838.668 kb on + strand, within BWI76_RS08725
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 1,835,711 - BWI76_RS08715 0.48 +0.1 1,835,788 + BWI76_RS08715 0.54 +0.6 1,836,021 + BWI76_RS08715 0.72 -1.0 1,836,029 - BWI76_RS08715 0.73 -1.7 1,836,096 + BWI76_RS08715 0.78 +0.5 1,836,330 - -0.2 1,836,334 + -0.7 1,836,434 - +0.7 1,836,585 + +0.7 1,836,688 - +0.2 1,836,688 - -2.8 1,837,299 + BWI76_RS08720 0.57 +0.4 1,837,602 + BWI76_RS08720 0.82 +1.7 1,837,653 - BWI76_RS08720 0.86 +1.6 1,837,885 - -0.1 1,838,181 + BWI76_RS08725 0.38 -0.3 1,838,229 + BWI76_RS08725 0.43 +0.1 1,838,484 - BWI76_RS08725 0.69 -3.4 1,838,668 + BWI76_RS08725 0.89 +0.1 1,838,668 + BWI76_RS08725 0.89 +1.4
Or see this region's nucleotide sequence