Experiment: Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01230 and BWI76_RS01235 are separated by 49 nucleotides BWI76_RS01235 and BWI76_RS01240 are separated by 155 nucleotides BWI76_RS01240 and BWI76_RS01245 are separated by 67 nucleotides
BWI76_RS01230: BWI76_RS01230 - chloramphenicol resistance permease RarD, at 252,092 to 252,985
_RS01230
BWI76_RS01235: BWI76_RS01235 - hypothetical protein, at 253,035 to 253,499
_RS01235
BWI76_RS01240: BWI76_RS01240 - phospholipase A, at 253,655 to 254,527
_RS01240
BWI76_RS01245: BWI76_RS01245 - DNA helicase RecQ, at 254,595 to 256,421
_RS01245
Position (kb) 253
254
255 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 253.077 kb on - strand at 253.154 kb on + strand, within BWI76_RS01235 at 253.273 kb on + strand, within BWI76_RS01235 at 253.538 kb on - strand at 253.615 kb on - strand at 253.657 kb on + strand at 253.664 kb on + strand at 253.664 kb on + strand at 253.672 kb on - strand at 253.672 kb on - strand at 253.743 kb on + strand, within BWI76_RS01240 at 253.815 kb on + strand, within BWI76_RS01240 at 253.823 kb on - strand, within BWI76_RS01240 at 253.939 kb on - strand, within BWI76_RS01240 at 254.025 kb on - strand, within BWI76_RS01240 at 254.123 kb on + strand, within BWI76_RS01240 at 254.316 kb on + strand, within BWI76_RS01240 at 254.331 kb on + strand, within BWI76_RS01240 at 254.339 kb on - strand, within BWI76_RS01240 at 254.427 kb on + strand, within BWI76_RS01240 at 254.437 kb on + strand, within BWI76_RS01240 at 254.639 kb on + strand at 254.703 kb on + strand at 254.722 kb on + strand at 254.722 kb on + strand at 254.769 kb on - strand at 254.831 kb on + strand, within BWI76_RS01245 at 254.901 kb on - strand, within BWI76_RS01245 at 255.222 kb on - strand, within BWI76_RS01245
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 253,077 - +1.2 253,154 + BWI76_RS01235 0.26 +1.1 253,273 + BWI76_RS01235 0.51 +0.3 253,538 - -0.5 253,615 - -0.9 253,657 + +1.8 253,664 + +1.3 253,664 + +0.7 253,672 - +2.5 253,672 - -0.1 253,743 + BWI76_RS01240 0.10 +0.3 253,815 + BWI76_RS01240 0.18 +1.0 253,823 - BWI76_RS01240 0.19 -3.3 253,939 - BWI76_RS01240 0.33 +2.1 254,025 - BWI76_RS01240 0.42 -1.5 254,123 + BWI76_RS01240 0.54 +1.1 254,316 + BWI76_RS01240 0.76 +0.8 254,331 + BWI76_RS01240 0.77 +0.9 254,339 - BWI76_RS01240 0.78 +1.3 254,427 + BWI76_RS01240 0.88 +1.1 254,437 + BWI76_RS01240 0.90 +1.1 254,639 + +1.0 254,703 + +1.8 254,722 + -1.5 254,722 + -2.5 254,769 - +0.4 254,831 + BWI76_RS01245 0.13 -1.7 254,901 - BWI76_RS01245 0.17 +0.1 255,222 - BWI76_RS01245 0.34 -0.4
Or see this region's nucleotide sequence