Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00275

Experiment: Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00265 and BWI76_RS00270 are separated by 182 nucleotidesBWI76_RS00270 and BWI76_RS00275 are separated by 10 nucleotidesBWI76_RS00275 and BWI76_RS00280 are separated by 5 nucleotidesBWI76_RS00280 and BWI76_RS00285 are separated by 27 nucleotides BWI76_RS00265: BWI76_RS00265 - potassium transporter Kup, at 54,258 to 56,126 _RS00265 BWI76_RS00270: BWI76_RS00270 - D-ribose pyranase (EC 5.4.99.62) (from data), at 56,309 to 56,728 _RS00270 BWI76_RS00275: BWI76_RS00275 - ribose ABC transporter ATP-binding protein RbsA, at 56,739 to 58,244 _RS00275 BWI76_RS00280: BWI76_RS00280 - ribose ABC transporter permease, at 58,250 to 59,215 _RS00280 BWI76_RS00285: BWI76_RS00285 - D-ribose ABC transporter substrate-binding protein, at 59,243 to 60,133 _RS00285 Position (kb) 56 57 58 59Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 55.745 kb on + strand, within BWI76_RS00265at 55.810 kb on + strand, within BWI76_RS00265at 55.932 kb on + strand, within BWI76_RS00265at 55.932 kb on + strand, within BWI76_RS00265at 55.940 kb on - strandat 56.062 kb on + strandat 56.370 kb on - strand, within BWI76_RS00270at 56.429 kb on + strand, within BWI76_RS00270at 56.435 kb on + strand, within BWI76_RS00270at 56.540 kb on - strand, within BWI76_RS00270at 56.568 kb on + strand, within BWI76_RS00270at 56.576 kb on - strand, within BWI76_RS00270at 56.576 kb on - strand, within BWI76_RS00270at 56.576 kb on - strand, within BWI76_RS00270at 56.578 kb on + strand, within BWI76_RS00270at 56.738 kb on + strandat 56.747 kb on - strandat 56.943 kb on + strand, within BWI76_RS00275at 57.201 kb on + strand, within BWI76_RS00275at 57.249 kb on - strand, within BWI76_RS00275at 57.329 kb on - strand, within BWI76_RS00275at 57.400 kb on - strand, within BWI76_RS00275at 57.553 kb on + strand, within BWI76_RS00275at 57.596 kb on + strand, within BWI76_RS00275at 57.628 kb on - strand, within BWI76_RS00275at 57.640 kb on - strand, within BWI76_RS00275at 57.661 kb on + strand, within BWI76_RS00275at 57.669 kb on - strand, within BWI76_RS00275at 57.726 kb on + strand, within BWI76_RS00275at 57.733 kb on + strand, within BWI76_RS00275at 57.764 kb on - strandat 57.770 kb on - strand, within BWI76_RS00275at 57.806 kb on + strand, within BWI76_RS00275at 57.814 kb on - strand, within BWI76_RS00275at 57.913 kb on + strand, within BWI76_RS00275at 58.020 kb on - strand, within BWI76_RS00275at 58.093 kb on + strand, within BWI76_RS00275at 58.101 kb on - strandat 58.129 kb on + strandat 58.395 kb on - strand, within BWI76_RS00280at 58.395 kb on - strand, within BWI76_RS00280at 58.442 kb on - strandat 58.490 kb on + strand, within BWI76_RS00280at 58.779 kb on + strand, within BWI76_RS00280at 58.819 kb on + strand, within BWI76_RS00280at 58.829 kb on + strand, within BWI76_RS00280at 59.158 kb on - strandat 59.173 kb on + strandat 59.215 kb on + strandat 59.231 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
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55,745 + BWI76_RS00265 0.80 -1.5
55,810 + BWI76_RS00265 0.83 -0.5
55,932 + BWI76_RS00265 0.90 -2.2
55,932 + BWI76_RS00265 0.90 -0.6
55,940 - -1.1
56,062 + +0.4
56,370 - BWI76_RS00270 0.15 -0.2
56,429 + BWI76_RS00270 0.29 +0.3
56,435 + BWI76_RS00270 0.30 -0.7
56,540 - BWI76_RS00270 0.55 +3.8
56,568 + BWI76_RS00270 0.62 +0.6
56,576 - BWI76_RS00270 0.64 +1.8
56,576 - BWI76_RS00270 0.64 -1.2
56,576 - BWI76_RS00270 0.64 +1.0
56,578 + BWI76_RS00270 0.64 +0.5
56,738 + -0.1
56,747 - +2.7
56,943 + BWI76_RS00275 0.14 -0.7
57,201 + BWI76_RS00275 0.31 +0.6
57,249 - BWI76_RS00275 0.34 -0.9
57,329 - BWI76_RS00275 0.39 -0.1
57,400 - BWI76_RS00275 0.44 +1.1
57,553 + BWI76_RS00275 0.54 +0.2
57,596 + BWI76_RS00275 0.57 +0.4
57,628 - BWI76_RS00275 0.59 -0.7
57,640 - BWI76_RS00275 0.60 -1.3
57,661 + BWI76_RS00275 0.61 +1.2
57,669 - BWI76_RS00275 0.62 +0.6
57,726 + BWI76_RS00275 0.66 +0.4
57,733 + BWI76_RS00275 0.66 +0.0
57,764 - -0.5
57,770 - BWI76_RS00275 0.68 -0.3
57,806 + BWI76_RS00275 0.71 -0.2
57,814 - BWI76_RS00275 0.71 +0.7
57,913 + BWI76_RS00275 0.78 +0.6
58,020 - BWI76_RS00275 0.85 -1.2
58,093 + BWI76_RS00275 0.90 +0.3
58,101 - -0.3
58,129 + +1.0
58,395 - BWI76_RS00280 0.15 +0.6
58,395 - BWI76_RS00280 0.15 +0.6
58,442 - +1.1
58,490 + BWI76_RS00280 0.25 -1.8
58,779 + BWI76_RS00280 0.55 +0.1
58,819 + BWI76_RS00280 0.59 +0.3
58,829 + BWI76_RS00280 0.60 -1.7
59,158 - -1.5
59,173 + -0.5
59,215 + +1.1
59,231 - -1.9

Or see this region's nucleotide sequence