Experiment: MoLS4 without Choline Chloride with Casaminos (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysC and DVUA0080 are separated by 97 nucleotides DVUA0080 and DVUA0081 overlap by 1 nucleotides DVUA0081 and xerD are separated by 304 nucleotides
DVUA0079: cysC - adenylylsulfate kinase (TIGR), at 105,582 to 106,268
cysC
DVUA0080: DVUA0080 - glycosyl transferase, group 2 family protein (TIGR), at 106,366 to 107,247
DVUA0080
DVUA0081: DVUA0081 - glycosyl transferase, group 1/2 family protein (TIGR), at 107,247 to 109,361
DVUA0081
DVUA0082: xerD - site-specific recombinase, phage integrase family (TIGR), at 109,666 to 110,697
xerD
Position (kb)
107
108
109
110 Strain fitness (log2 ratio)
-1
0
1
2 at 106.367 kb on + strand at 106.439 kb on + strand at 106.575 kb on + strand, within DVUA0080 at 106.593 kb on - strand, within DVUA0080 at 106.620 kb on - strand, within DVUA0080 at 106.622 kb on + strand, within DVUA0080 at 107.110 kb on + strand, within DVUA0080 at 107.334 kb on + strand at 107.342 kb on - strand at 107.362 kb on + strand at 107.448 kb on - strand at 107.736 kb on + strand, within DVUA0081 at 107.851 kb on + strand, within DVUA0081 at 108.049 kb on + strand, within DVUA0081 at 108.285 kb on + strand, within DVUA0081 at 108.366 kb on + strand, within DVUA0081 at 108.387 kb on + strand, within DVUA0081 at 108.454 kb on + strand, within DVUA0081 at 108.616 kb on + strand, within DVUA0081 at 108.949 kb on + strand, within DVUA0081 at 109.181 kb on + strand at 109.189 kb on - strand at 109.189 kb on - strand at 109.376 kb on - strand at 109.376 kb on - strand at 109.376 kb on - strand at 109.382 kb on - strand at 109.388 kb on + strand at 109.440 kb on - strand at 109.871 kb on + strand, within xerD at 109.879 kb on - strand, within xerD at 109.913 kb on + strand, within xerD at 109.921 kb on - strand, within xerD at 109.991 kb on + strand, within xerD at 109.999 kb on - strand, within xerD at 109.999 kb on - strand, within xerD at 110.006 kb on + strand, within xerD at 110.014 kb on - strand, within xerD at 110.017 kb on - strand, within xerD at 110.311 kb on + strand, within xerD
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without Choline Chloride with Casaminos (vit. assay) 0.05% remove 106,367 + +0.8 106,439 + -0.4 106,575 + DVUA0080 0.24 +1.4 106,593 - DVUA0080 0.26 -0.2 106,620 - DVUA0080 0.29 +0.2 106,622 + DVUA0080 0.29 +1.4 107,110 + DVUA0080 0.84 -1.5 107,334 + +0.7 107,342 - +0.8 107,362 + +0.3 107,448 - +0.8 107,736 + DVUA0081 0.23 -1.4 107,851 + DVUA0081 0.29 +0.1 108,049 + DVUA0081 0.38 -0.2 108,285 + DVUA0081 0.49 -0.2 108,366 + DVUA0081 0.53 -0.4 108,387 + DVUA0081 0.54 +1.4 108,454 + DVUA0081 0.57 +0.7 108,616 + DVUA0081 0.65 +2.4 108,949 + DVUA0081 0.80 -0.2 109,181 + -1.4 109,189 - +0.4 109,189 - +1.4 109,376 - -0.9 109,376 - -0.3 109,376 - +0.1 109,382 - +0.4 109,388 + -0.5 109,440 - -0.3 109,871 + xerD DVUA0082 0.20 +0.1 109,879 - xerD DVUA0082 0.21 -0.5 109,913 + xerD DVUA0082 0.24 -1.0 109,921 - xerD DVUA0082 0.25 -0.0 109,991 + xerD DVUA0082 0.31 -0.4 109,999 - xerD DVUA0082 0.32 -0.3 109,999 - xerD DVUA0082 0.32 +1.7 110,006 + xerD DVUA0082 0.33 -0.1 110,014 - xerD DVUA0082 0.34 +0.0 110,017 - xerD DVUA0082 0.34 +0.1 110,311 + xerD DVUA0082 0.62 -0.2
Or see this region's nucleotide sequence