Experiment: MoLS4 without Choline Chloride with Casaminos (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trpC and trpF-1 are separated by 47 nucleotides trpF-1 and trpB-2 overlap by 4 nucleotides trpB-2 and trpA overlap by 4 nucleotides
DVU0468: trpC - indole-3-glycerol phosphate synthase (TIGR), at 536,049 to 536,822
trpC
DVU0469: trpF-1 - N-(5-phosphoribosyl)anthranilate isomerase (TIGR), at 536,870 to 537,724
trpF-1
DVU0470: trpB-2 - tryptophan synthase, beta subunit (TIGR), at 537,721 to 538,908
trpB-2
DVU0471: trpA - tryptophan synthase, alpha subunit (TIGR), at 538,905 to 539,678
trpA
Position (kb) 537
538
539 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1 at 536.750 kb on - strand at 536.757 kb on + strand at 536.757 kb on + strand at 536.765 kb on - strand at 536.799 kb on + strand at 536.807 kb on - strand at 536.860 kb on + strand at 536.979 kb on + strand, within trpF-1 at 537.037 kb on - strand, within trpF-1 at 537.037 kb on - strand, within trpF-1 at 537.044 kb on - strand, within trpF-1 at 537.044 kb on - strand, within trpF-1 at 537.135 kb on + strand, within trpF-1 at 537.206 kb on - strand, within trpF-1 at 537.288 kb on + strand, within trpF-1 at 537.389 kb on - strand, within trpF-1 at 537.476 kb on + strand, within trpF-1 at 537.479 kb on - strand, within trpF-1 at 537.489 kb on - strand, within trpF-1 at 537.574 kb on - strand, within trpF-1 at 537.579 kb on - strand, within trpF-1 at 537.664 kb on - strand at 537.671 kb on - strand at 537.688 kb on - strand at 537.690 kb on + strand at 537.692 kb on + strand at 538.023 kb on - strand, within trpB-2 at 538.233 kb on + strand, within trpB-2 at 538.236 kb on - strand, within trpB-2 at 538.244 kb on - strand, within trpB-2 at 538.254 kb on + strand, within trpB-2 at 538.264 kb on + strand, within trpB-2 at 538.432 kb on + strand, within trpB-2 at 538.527 kb on - strand, within trpB-2 at 538.529 kb on - strand, within trpB-2 at 538.594 kb on - strand, within trpB-2 at 538.814 kb on + strand at 538.825 kb on - strand at 538.855 kb on + strand at 538.863 kb on - strand at 538.863 kb on - strand at 538.873 kb on + strand at 539.142 kb on - strand, within trpA at 539.152 kb on - strand, within trpA at 539.178 kb on + strand, within trpA at 539.194 kb on + strand, within trpA at 539.202 kb on - strand, within trpA at 539.202 kb on - strand, within trpA at 539.202 kb on - strand, within trpA at 539.213 kb on + strand, within trpA at 539.279 kb on - strand, within trpA at 539.366 kb on + strand, within trpA at 539.371 kb on + strand, within trpA at 539.434 kb on - strand, within trpA at 539.450 kb on - strand, within trpA at 539.618 kb on - strand at 539.812 kb on + strand at 539.872 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without Choline Chloride with Casaminos (vit. assay) 0.05% remove 536,750 - -2.8 536,757 + -2.5 536,757 + -1.4 536,765 - -4.6 536,799 + -1.0 536,807 - -3.9 536,860 + -3.8 536,979 + trpF-1 DVU0469 0.13 -4.6 537,037 - trpF-1 DVU0469 0.20 -2.3 537,037 - trpF-1 DVU0469 0.20 -3.8 537,044 - trpF-1 DVU0469 0.20 -3.9 537,044 - trpF-1 DVU0469 0.20 -7.3 537,135 + trpF-1 DVU0469 0.31 -4.9 537,206 - trpF-1 DVU0469 0.39 -1.1 537,288 + trpF-1 DVU0469 0.49 -3.7 537,389 - trpF-1 DVU0469 0.61 -5.3 537,476 + trpF-1 DVU0469 0.71 +0.1 537,479 - trpF-1 DVU0469 0.71 -2.5 537,489 - trpF-1 DVU0469 0.72 +0.1 537,574 - trpF-1 DVU0469 0.82 -3.8 537,579 - trpF-1 DVU0469 0.83 -0.8 537,664 - +0.2 537,671 - -0.1 537,688 - +0.2 537,690 + +0.3 537,692 + +0.3 538,023 - trpB-2 DVU0470 0.25 -1.8 538,233 + trpB-2 DVU0470 0.43 -3.5 538,236 - trpB-2 DVU0470 0.43 -0.8 538,244 - trpB-2 DVU0470 0.44 -3.0 538,254 + trpB-2 DVU0470 0.45 -1.1 538,264 + trpB-2 DVU0470 0.46 -3.1 538,432 + trpB-2 DVU0470 0.60 -1.3 538,527 - trpB-2 DVU0470 0.68 -1.8 538,529 - trpB-2 DVU0470 0.68 -5.3 538,594 - trpB-2 DVU0470 0.73 -3.9 538,814 + -3.5 538,825 - -1.6 538,855 + +1.4 538,863 - -0.7 538,863 - -0.3 538,873 + -0.5 539,142 - trpA DVU0471 0.31 -3.4 539,152 - trpA DVU0471 0.32 -4.0 539,178 + trpA DVU0471 0.35 -2.1 539,194 + trpA DVU0471 0.37 -2.6 539,202 - trpA DVU0471 0.38 -6.0 539,202 - trpA DVU0471 0.38 -5.1 539,202 - trpA DVU0471 0.38 -0.4 539,213 + trpA DVU0471 0.40 -6.1 539,279 - trpA DVU0471 0.48 -5.8 539,366 + trpA DVU0471 0.60 -3.1 539,371 + trpA DVU0471 0.60 +0.1 539,434 - trpA DVU0471 0.68 -3.9 539,450 - trpA DVU0471 0.70 -1.7 539,618 - -1.1 539,812 + +0.5 539,872 - -0.1
Or see this region's nucleotide sequence