Experiment: MoLS4 without Choline Chloride with Casaminos (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trpE and trpG overlap by 20 nucleotides trpG and trpD overlap by 4 nucleotides trpD and trpC overlap by 8 nucleotides trpC and trpF-1 are separated by 47 nucleotides
DVU0465: trpE - anthranilate synthase, component I (TIGR), at 532,853 to 534,451
trpE
DVU0466: trpG - anthranilate synthase, glutamine amidotransferase component (TIGR), at 534,432 to 535,061
trpG
DVU0467: trpD - anthranilate phosphoribosyltransferase (TIGR), at 535,058 to 536,056
trpD
DVU0468: trpC - indole-3-glycerol phosphate synthase (TIGR), at 536,049 to 536,822
trpC
DVU0469: trpF-1 - N-(5-phosphoribosyl)anthranilate isomerase (TIGR), at 536,870 to 537,724
trpF-1
Position (kb) 535
536
537 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1 at 534.073 kb on + strand, within trpE at 534.090 kb on + strand, within trpE at 534.098 kb on - strand, within trpE at 534.107 kb on + strand, within trpE at 534.149 kb on + strand, within trpE at 534.180 kb on - strand, within trpE at 534.256 kb on - strand, within trpE at 534.322 kb on - strand at 534.382 kb on + strand at 534.418 kb on + strand at 534.466 kb on + strand at 534.490 kb on - strand at 534.500 kb on - strand, within trpG at 534.501 kb on - strand, within trpG at 534.501 kb on - strand, within trpG at 534.563 kb on + strand, within trpG at 534.596 kb on - strand, within trpG at 534.717 kb on - strand, within trpG at 534.766 kb on + strand, within trpG at 534.796 kb on + strand, within trpG at 534.807 kb on + strand, within trpG at 534.895 kb on - strand, within trpG at 534.905 kb on + strand, within trpG at 534.921 kb on - strand, within trpG at 534.935 kb on - strand, within trpG at 535.038 kb on - strand at 535.103 kb on + strand at 535.130 kb on + strand at 535.204 kb on + strand, within trpD at 535.206 kb on - strand, within trpD at 535.229 kb on - strand, within trpD at 535.233 kb on - strand, within trpD at 535.239 kb on + strand, within trpD at 535.244 kb on + strand, within trpD at 535.244 kb on + strand, within trpD at 535.244 kb on + strand, within trpD at 535.244 kb on + strand, within trpD at 535.244 kb on + strand, within trpD at 535.252 kb on - strand, within trpD at 535.252 kb on - strand, within trpD at 535.252 kb on - strand, within trpD at 535.252 kb on - strand, within trpD at 535.257 kb on - strand, within trpD at 535.272 kb on + strand, within trpD at 535.447 kb on - strand, within trpD at 535.508 kb on - strand, within trpD at 535.546 kb on + strand, within trpD at 535.591 kb on - strand, within trpD at 535.634 kb on - strand, within trpD at 535.709 kb on + strand, within trpD at 535.756 kb on - strand, within trpD at 535.839 kb on + strand, within trpD at 535.841 kb on + strand, within trpD at 535.841 kb on + strand, within trpD at 535.849 kb on - strand, within trpD at 535.871 kb on - strand, within trpD at 535.897 kb on + strand, within trpD at 535.905 kb on - strand, within trpD at 535.920 kb on - strand, within trpD at 535.938 kb on + strand, within trpD at 535.942 kb on - strand, within trpD at 535.942 kb on - strand, within trpD at 535.955 kb on + strand, within trpD at 535.955 kb on + strand, within trpD at 535.963 kb on - strand at 535.983 kb on + strand at 535.985 kb on + strand at 535.985 kb on + strand at 535.985 kb on + strand at 536.095 kb on - strand at 536.158 kb on + strand, within trpC at 536.164 kb on - strand, within trpC at 536.166 kb on - strand, within trpC at 536.166 kb on - strand, within trpC at 536.179 kb on + strand, within trpC at 536.179 kb on + strand, within trpC at 536.179 kb on + strand, within trpC at 536.301 kb on - strand, within trpC at 536.303 kb on + strand, within trpC at 536.311 kb on - strand, within trpC at 536.318 kb on + strand, within trpC at 536.318 kb on + strand, within trpC at 536.369 kb on - strand, within trpC at 536.425 kb on + strand, within trpC at 536.461 kb on + strand, within trpC at 536.467 kb on + strand, within trpC at 536.506 kb on - strand, within trpC at 536.511 kb on + strand, within trpC at 536.519 kb on - strand, within trpC at 536.519 kb on - strand, within trpC at 536.547 kb on - strand, within trpC at 536.566 kb on + strand, within trpC at 536.611 kb on + strand, within trpC at 536.619 kb on - strand, within trpC at 536.669 kb on + strand, within trpC at 536.750 kb on - strand at 536.757 kb on + strand at 536.757 kb on + strand at 536.765 kb on - strand at 536.799 kb on + strand at 536.807 kb on - strand at 536.860 kb on + strand at 536.979 kb on + strand, within trpF-1 at 537.037 kb on - strand, within trpF-1 at 537.037 kb on - strand, within trpF-1 at 537.044 kb on - strand, within trpF-1 at 537.044 kb on - strand, within trpF-1
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without Choline Chloride with Casaminos (vit. assay) 0.05% remove 534,073 + trpE DVU0465 0.76 -2.5 534,090 + trpE DVU0465 0.77 -3.4 534,098 - trpE DVU0465 0.78 -1.8 534,107 + trpE DVU0465 0.78 -4.2 534,149 + trpE DVU0465 0.81 -2.6 534,180 - trpE DVU0465 0.83 -4.0 534,256 - trpE DVU0465 0.88 -4.5 534,322 - -5.5 534,382 + -2.1 534,418 + -0.4 534,466 + -2.1 534,490 - -0.6 534,500 - trpG DVU0466 0.11 -3.0 534,501 - trpG DVU0466 0.11 -2.2 534,501 - trpG DVU0466 0.11 -3.5 534,563 + trpG DVU0466 0.21 -5.3 534,596 - trpG DVU0466 0.26 -4.5 534,717 - trpG DVU0466 0.45 -0.8 534,766 + trpG DVU0466 0.53 +0.4 534,796 + trpG DVU0466 0.58 -4.0 534,807 + trpG DVU0466 0.60 -3.5 534,895 - trpG DVU0466 0.73 -4.2 534,905 + trpG DVU0466 0.75 -1.3 534,921 - trpG DVU0466 0.78 -3.0 534,935 - trpG DVU0466 0.80 -1.1 535,038 - -2.1 535,103 + -6.2 535,130 + -3.2 535,204 + trpD DVU0467 0.15 -4.4 535,206 - trpD DVU0467 0.15 -3.9 535,229 - trpD DVU0467 0.17 -3.6 535,233 - trpD DVU0467 0.18 -4.5 535,239 + trpD DVU0467 0.18 -5.5 535,244 + trpD DVU0467 0.19 -5.2 535,244 + trpD DVU0467 0.19 -3.0 535,244 + trpD DVU0467 0.19 -4.7 535,244 + trpD DVU0467 0.19 -1.2 535,244 + trpD DVU0467 0.19 -4.9 535,252 - trpD DVU0467 0.19 -5.3 535,252 - trpD DVU0467 0.19 -0.8 535,252 - trpD DVU0467 0.19 -2.2 535,252 - trpD DVU0467 0.19 -1.1 535,257 - trpD DVU0467 0.20 -3.6 535,272 + trpD DVU0467 0.21 -4.7 535,447 - trpD DVU0467 0.39 -3.1 535,508 - trpD DVU0467 0.45 -4.4 535,546 + trpD DVU0467 0.49 -4.4 535,591 - trpD DVU0467 0.53 -3.7 535,634 - trpD DVU0467 0.58 -1.8 535,709 + trpD DVU0467 0.65 -2.7 535,756 - trpD DVU0467 0.70 -1.6 535,839 + trpD DVU0467 0.78 -2.3 535,841 + trpD DVU0467 0.78 -3.4 535,841 + trpD DVU0467 0.78 -0.4 535,849 - trpD DVU0467 0.79 -3.2 535,871 - trpD DVU0467 0.81 -4.4 535,897 + trpD DVU0467 0.84 -2.8 535,905 - trpD DVU0467 0.85 -3.6 535,920 - trpD DVU0467 0.86 -4.2 535,938 + trpD DVU0467 0.88 -2.8 535,942 - trpD DVU0467 0.88 -0.8 535,942 - trpD DVU0467 0.88 -0.6 535,955 + trpD DVU0467 0.90 -1.8 535,955 + trpD DVU0467 0.90 -4.3 535,963 - -4.9 535,983 + -1.1 535,985 + -6.0 535,985 + -0.2 535,985 + -4.0 536,095 - -4.7 536,158 + trpC DVU0468 0.14 -5.5 536,164 - trpC DVU0468 0.15 -0.7 536,166 - trpC DVU0468 0.15 -2.7 536,166 - trpC DVU0468 0.15 -6.1 536,179 + trpC DVU0468 0.17 -4.7 536,179 + trpC DVU0468 0.17 -5.5 536,179 + trpC DVU0468 0.17 -4.3 536,301 - trpC DVU0468 0.33 -3.3 536,303 + trpC DVU0468 0.33 -0.2 536,311 - trpC DVU0468 0.34 -6.1 536,318 + trpC DVU0468 0.35 -4.9 536,318 + trpC DVU0468 0.35 -3.3 536,369 - trpC DVU0468 0.41 -3.6 536,425 + trpC DVU0468 0.49 -6.9 536,461 + trpC DVU0468 0.53 -5.4 536,467 + trpC DVU0468 0.54 -1.3 536,506 - trpC DVU0468 0.59 -4.9 536,511 + trpC DVU0468 0.60 -6.5 536,519 - trpC DVU0468 0.61 -2.5 536,519 - trpC DVU0468 0.61 -3.2 536,547 - trpC DVU0468 0.64 +0.4 536,566 + trpC DVU0468 0.67 -2.6 536,611 + trpC DVU0468 0.73 -5.1 536,619 - trpC DVU0468 0.74 -6.4 536,669 + trpC DVU0468 0.80 -2.5 536,750 - -2.8 536,757 + -2.5 536,757 + -1.4 536,765 - -4.6 536,799 + -1.0 536,807 - -3.9 536,860 + -3.8 536,979 + trpF-1 DVU0469 0.13 -4.6 537,037 - trpF-1 DVU0469 0.20 -2.3 537,037 - trpF-1 DVU0469 0.20 -3.8 537,044 - trpF-1 DVU0469 0.20 -3.9 537,044 - trpF-1 DVU0469 0.20 -7.3
Or see this region's nucleotide sequence