Experiment: MoLS4 without Choline Chloride with Casaminos (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aroA and DVU0464 are separated by 5 nucleotides DVU0464 and trpE are separated by 131 nucleotides trpE and trpG overlap by 20 nucleotides trpG and trpD overlap by 4 nucleotides
DVU0463: aroA - 3-phosphoshikimate 1-carboxyvinyltransferase (TIGR), at 530,629 to 531,948
aroA
DVU0464: DVU0464 - prephenate and/or arogenate dehydrogenase <small>(from data)</small>, at 531,954 to 532,721
DVU0464
DVU0465: trpE - anthranilate synthase, component I (TIGR), at 532,853 to 534,451
trpE
DVU0466: trpG - anthranilate synthase, glutamine amidotransferase component (TIGR), at 534,432 to 535,061
trpG
DVU0467: trpD - anthranilate phosphoribosyltransferase (TIGR), at 535,058 to 536,056
trpD
Position (kb) 532
533
534
535 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 531.925 kb on + strand at 531.986 kb on + strand at 532.039 kb on + strand, within DVU0464 at 532.087 kb on + strand, within DVU0464 at 532.260 kb on - strand, within DVU0464 at 532.347 kb on + strand, within DVU0464 at 532.436 kb on - strand, within DVU0464 at 532.436 kb on - strand, within DVU0464 at 532.552 kb on + strand, within DVU0464 at 532.557 kb on + strand, within DVU0464 at 532.678 kb on + strand at 532.692 kb on + strand at 532.700 kb on - strand at 532.722 kb on - strand at 532.737 kb on + strand at 532.844 kb on + strand at 532.917 kb on - strand at 532.938 kb on + strand at 532.948 kb on + strand at 532.951 kb on - strand at 532.956 kb on - strand at 532.961 kb on - strand at 533.078 kb on - strand, within trpE at 533.129 kb on + strand, within trpE at 533.153 kb on + strand, within trpE at 533.220 kb on + strand, within trpE at 533.286 kb on - strand, within trpE at 533.374 kb on - strand, within trpE at 533.461 kb on + strand, within trpE at 533.466 kb on + strand, within trpE at 533.489 kb on - strand, within trpE at 533.512 kb on + strand, within trpE at 533.547 kb on - strand, within trpE at 533.554 kb on - strand, within trpE at 533.608 kb on - strand, within trpE at 533.613 kb on - strand, within trpE at 533.615 kb on + strand, within trpE at 533.615 kb on + strand, within trpE at 533.623 kb on - strand, within trpE at 533.651 kb on - strand, within trpE at 533.687 kb on - strand, within trpE at 533.715 kb on - strand, within trpE at 533.719 kb on + strand, within trpE at 533.741 kb on - strand, within trpE at 533.743 kb on + strand, within trpE at 533.751 kb on - strand, within trpE at 533.769 kb on - strand, within trpE at 533.774 kb on + strand, within trpE at 533.871 kb on - strand, within trpE at 534.030 kb on - strand, within trpE at 534.037 kb on + strand, within trpE at 534.073 kb on + strand, within trpE at 534.090 kb on + strand, within trpE at 534.098 kb on - strand, within trpE at 534.107 kb on + strand, within trpE at 534.149 kb on + strand, within trpE at 534.180 kb on - strand, within trpE at 534.256 kb on - strand, within trpE at 534.322 kb on - strand at 534.382 kb on + strand at 534.418 kb on + strand at 534.466 kb on + strand at 534.490 kb on - strand at 534.500 kb on - strand, within trpG at 534.501 kb on - strand, within trpG at 534.501 kb on - strand, within trpG at 534.563 kb on + strand, within trpG at 534.596 kb on - strand, within trpG at 534.717 kb on - strand, within trpG at 534.766 kb on + strand, within trpG at 534.796 kb on + strand, within trpG at 534.807 kb on + strand, within trpG at 534.895 kb on - strand, within trpG at 534.905 kb on + strand, within trpG at 534.921 kb on - strand, within trpG at 534.935 kb on - strand, within trpG at 535.038 kb on - strand at 535.103 kb on + strand at 535.130 kb on + strand at 535.204 kb on + strand, within trpD at 535.206 kb on - strand, within trpD at 535.229 kb on - strand, within trpD at 535.233 kb on - strand, within trpD at 535.239 kb on + strand, within trpD at 535.244 kb on + strand, within trpD at 535.244 kb on + strand, within trpD at 535.244 kb on + strand, within trpD at 535.244 kb on + strand, within trpD at 535.244 kb on + strand, within trpD at 535.252 kb on - strand, within trpD at 535.252 kb on - strand, within trpD at 535.252 kb on - strand, within trpD at 535.252 kb on - strand, within trpD at 535.257 kb on - strand, within trpD at 535.272 kb on + strand, within trpD at 535.447 kb on - strand, within trpD
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without Choline Chloride with Casaminos (vit. assay) 0.05% remove 531,925 + -3.4 531,986 + -1.4 532,039 + DVU0464 0.11 -0.3 532,087 + DVU0464 0.17 -1.5 532,260 - DVU0464 0.40 -1.6 532,347 + DVU0464 0.51 -1.1 532,436 - DVU0464 0.63 -0.6 532,436 - DVU0464 0.63 -3.3 532,552 + DVU0464 0.78 -2.2 532,557 + DVU0464 0.79 +1.7 532,678 + -0.1 532,692 + -0.0 532,700 - +0.7 532,722 - -4.2 532,737 + -2.8 532,844 + +0.7 532,917 - -5.6 532,938 + +0.2 532,948 + -0.4 532,951 - -2.7 532,956 - -4.5 532,961 - -4.7 533,078 - trpE DVU0465 0.14 -1.8 533,129 + trpE DVU0465 0.17 -0.4 533,153 + trpE DVU0465 0.19 -2.9 533,220 + trpE DVU0465 0.23 -2.4 533,286 - trpE DVU0465 0.27 -3.8 533,374 - trpE DVU0465 0.33 -4.0 533,461 + trpE DVU0465 0.38 -5.4 533,466 + trpE DVU0465 0.38 -3.5 533,489 - trpE DVU0465 0.40 -4.1 533,512 + trpE DVU0465 0.41 -4.9 533,547 - trpE DVU0465 0.43 -0.1 533,554 - trpE DVU0465 0.44 -3.9 533,608 - trpE DVU0465 0.47 -4.7 533,613 - trpE DVU0465 0.48 -1.9 533,615 + trpE DVU0465 0.48 -1.9 533,615 + trpE DVU0465 0.48 -4.3 533,623 - trpE DVU0465 0.48 -1.7 533,651 - trpE DVU0465 0.50 -1.8 533,687 - trpE DVU0465 0.52 -5.2 533,715 - trpE DVU0465 0.54 -3.3 533,719 + trpE DVU0465 0.54 -3.6 533,741 - trpE DVU0465 0.56 -6.5 533,743 + trpE DVU0465 0.56 -3.3 533,751 - trpE DVU0465 0.56 -1.6 533,769 - trpE DVU0465 0.57 -4.4 533,774 + trpE DVU0465 0.58 -2.8 533,871 - trpE DVU0465 0.64 -5.6 534,030 - trpE DVU0465 0.74 -5.4 534,037 + trpE DVU0465 0.74 -0.2 534,073 + trpE DVU0465 0.76 -2.5 534,090 + trpE DVU0465 0.77 -3.4 534,098 - trpE DVU0465 0.78 -1.8 534,107 + trpE DVU0465 0.78 -4.2 534,149 + trpE DVU0465 0.81 -2.6 534,180 - trpE DVU0465 0.83 -4.0 534,256 - trpE DVU0465 0.88 -4.5 534,322 - -5.5 534,382 + -2.1 534,418 + -0.4 534,466 + -2.1 534,490 - -0.6 534,500 - trpG DVU0466 0.11 -3.0 534,501 - trpG DVU0466 0.11 -2.2 534,501 - trpG DVU0466 0.11 -3.5 534,563 + trpG DVU0466 0.21 -5.3 534,596 - trpG DVU0466 0.26 -4.5 534,717 - trpG DVU0466 0.45 -0.8 534,766 + trpG DVU0466 0.53 +0.4 534,796 + trpG DVU0466 0.58 -4.0 534,807 + trpG DVU0466 0.60 -3.5 534,895 - trpG DVU0466 0.73 -4.2 534,905 + trpG DVU0466 0.75 -1.3 534,921 - trpG DVU0466 0.78 -3.0 534,935 - trpG DVU0466 0.80 -1.1 535,038 - -2.1 535,103 + -6.2 535,130 + -3.2 535,204 + trpD DVU0467 0.15 -4.4 535,206 - trpD DVU0467 0.15 -3.9 535,229 - trpD DVU0467 0.17 -3.6 535,233 - trpD DVU0467 0.18 -4.5 535,239 + trpD DVU0467 0.18 -5.5 535,244 + trpD DVU0467 0.19 -5.2 535,244 + trpD DVU0467 0.19 -3.0 535,244 + trpD DVU0467 0.19 -4.7 535,244 + trpD DVU0467 0.19 -1.2 535,244 + trpD DVU0467 0.19 -4.9 535,252 - trpD DVU0467 0.19 -5.3 535,252 - trpD DVU0467 0.19 -0.8 535,252 - trpD DVU0467 0.19 -2.2 535,252 - trpD DVU0467 0.19 -1.1 535,257 - trpD DVU0467 0.20 -3.6 535,272 + trpD DVU0467 0.21 -4.7 535,447 - trpD DVU0467 0.39 -3.1
Or see this region's nucleotide sequence