Experiment: MoLS4 without Choline Chloride with Casaminos (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt b2488 and DVU2290 overlap by 4 nucleotides DVU2290 and DVU2291 overlap by 4 nucleotides DVU2291 and hypA are separated by 8 nucleotides hypA and cooF overlap by 4 nucleotides
DVU2289: b2488 - hydrogenase, CooX subunit, putative (TIGR), at 2,382,468 to 2,383,103
b2488
DVU2290: DVU2290 - hydrogenase, CooU subunit, putative (TIGR), at 2,383,100 to 2,383,645
DVU2290
DVU2291: DVU2291 - carbon monoxide-induced hydrogenase CooH, putative (TIGR), at 2,383,642 to 2,384,742
DVU2291
DVU2292: hypA - hydrogenase nickel insertion protein HypA (TIGR), at 2,384,751 to 2,385,113
hypA
DVU2293: cooF - iron-sulfur protein CooF (TIGR), at 2,385,110 to 2,385,631
cooF
Position (kb)
2383
2384
2385 Strain fitness (log2 ratio)
-2
-1
0
1 at 2382.750 kb on - strand, within b2488 at 2382.926 kb on + strand, within b2488 at 2383.041 kb on + strand at 2383.041 kb on + strand at 2383.041 kb on + strand at 2383.116 kb on + strand at 2383.243 kb on + strand, within DVU2290 at 2383.365 kb on + strand, within DVU2290 at 2383.639 kb on + strand at 2383.775 kb on + strand, within DVU2291 at 2383.819 kb on - strand, within DVU2291 at 2384.062 kb on + strand, within DVU2291 at 2384.276 kb on - strand, within DVU2291 at 2384.352 kb on + strand, within DVU2291 at 2384.556 kb on + strand, within DVU2291 at 2384.716 kb on - strand at 2384.850 kb on + strand, within hypA at 2384.863 kb on - strand, within hypA at 2384.876 kb on + strand, within hypA at 2384.949 kb on + strand, within hypA at 2385.084 kb on - strand at 2385.304 kb on + strand, within cooF at 2385.519 kb on + strand, within cooF at 2385.568 kb on + strand, within cooF at 2385.592 kb on - strand at 2385.639 kb on - strand at 2385.702 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without Choline Chloride with Casaminos (vit. assay) 0.05% remove 2,382,750 - b2488 DVU2289 0.44 +1.7 2,382,926 + b2488 DVU2289 0.72 +0.4 2,383,041 + -0.7 2,383,041 + -1.0 2,383,041 + +0.2 2,383,116 + +0.0 2,383,243 + DVU2290 0.26 -0.3 2,383,365 + DVU2290 0.49 -0.3 2,383,639 + +0.8 2,383,775 + DVU2291 0.12 -1.7 2,383,819 - DVU2291 0.16 +0.2 2,384,062 + DVU2291 0.38 -0.7 2,384,276 - DVU2291 0.58 +0.7 2,384,352 + DVU2291 0.64 -0.5 2,384,556 + DVU2291 0.83 -2.4 2,384,716 - +1.5 2,384,850 + hypA DVU2292 0.27 -1.1 2,384,863 - hypA DVU2292 0.31 +0.7 2,384,876 + hypA DVU2292 0.34 -0.9 2,384,949 + hypA DVU2292 0.55 -1.9 2,385,084 - -0.3 2,385,304 + cooF DVU2293 0.37 +0.0 2,385,519 + cooF DVU2293 0.78 -0.1 2,385,568 + cooF DVU2293 0.88 -1.9 2,385,592 - -0.1 2,385,639 - -0.5 2,385,702 + -0.7
Or see this region's nucleotide sequence