Experiment: MoLS4 without Choline Chloride with Casaminos (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU1644 and DVU1645 are separated by 5 nucleotides DVU1645 and arsC are separated by 158 nucleotides arsC and lysA-1 are separated by 225 nucleotides
DVU1644: DVU1644 - permease, putative (TIGR), at 1,723,708 to 1,724,823
DVU1644
DVU1645: DVU1645 - transcriptional regulator, ArsR family (TIGR), at 1,724,829 to 1,725,173
DVU1645
DVU1646: arsC - arsenate reductase (TIGR), at 1,725,332 to 1,725,775
arsC
DVU1647: lysA-1 - diaminopimelate decarboxylase (TIGR), at 1,726,001 to 1,727,239
lysA-1
Position (kb)
1725
1726 Strain fitness (log2 ratio)
-2
-1
0
1 at 1724.351 kb on + strand, within DVU1644 at 1724.356 kb on + strand, within DVU1644 at 1724.399 kb on + strand, within DVU1644 at 1724.399 kb on - strand, within DVU1644 at 1724.554 kb on + strand, within DVU1644 at 1724.562 kb on - strand, within DVU1644 at 1724.567 kb on + strand, within DVU1644 at 1725.027 kb on + strand, within DVU1645 at 1725.244 kb on + strand at 1725.365 kb on + strand at 1725.373 kb on - strand at 1725.389 kb on + strand, within arsC at 1725.457 kb on + strand, within arsC at 1725.459 kb on + strand, within arsC at 1725.631 kb on - strand, within arsC at 1725.719 kb on + strand, within arsC at 1725.856 kb on + strand at 1725.861 kb on - strand at 1725.919 kb on + strand at 1725.951 kb on - strand at 1725.966 kb on + strand at 1725.974 kb on - strand at 1726.029 kb on - strand at 1726.243 kb on + strand, within lysA-1 at 1726.246 kb on - strand, within lysA-1 at 1726.260 kb on - strand, within lysA-1 at 1726.417 kb on - strand, within lysA-1 at 1726.459 kb on + strand, within lysA-1 at 1726.542 kb on - strand, within lysA-1 at 1726.631 kb on - strand, within lysA-1 at 1726.699 kb on - strand, within lysA-1 at 1726.705 kb on - strand, within lysA-1 at 1726.757 kb on + strand, within lysA-1 at 1726.774 kb on + strand, within lysA-1
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without Choline Chloride with Casaminos (vit. assay) 0.05% remove 1,724,351 + DVU1644 0.58 -0.2 1,724,356 + DVU1644 0.58 +0.2 1,724,399 + DVU1644 0.62 -0.4 1,724,399 - DVU1644 0.62 +1.0 1,724,554 + DVU1644 0.76 -0.3 1,724,562 - DVU1644 0.77 +0.1 1,724,567 + DVU1644 0.77 -2.0 1,725,027 + DVU1645 0.57 -0.6 1,725,244 + +0.1 1,725,365 + +1.3 1,725,373 - -0.5 1,725,389 + arsC DVU1646 0.13 -1.1 1,725,457 + arsC DVU1646 0.28 +0.0 1,725,459 + arsC DVU1646 0.29 +0.0 1,725,631 - arsC DVU1646 0.67 +0.2 1,725,719 + arsC DVU1646 0.87 -0.2 1,725,856 + +1.1 1,725,861 - -0.3 1,725,919 + +0.0 1,725,951 - -1.6 1,725,966 + +0.2 1,725,974 - -0.4 1,726,029 - -0.1 1,726,243 + lysA-1 DVU1647 0.20 +0.3 1,726,246 - lysA-1 DVU1647 0.20 +0.7 1,726,260 - lysA-1 DVU1647 0.21 -0.1 1,726,417 - lysA-1 DVU1647 0.34 -0.4 1,726,459 + lysA-1 DVU1647 0.37 -0.1 1,726,542 - lysA-1 DVU1647 0.44 +0.4 1,726,631 - lysA-1 DVU1647 0.51 +0.0 1,726,699 - lysA-1 DVU1647 0.56 -0.1 1,726,705 - lysA-1 DVU1647 0.57 +0.0 1,726,757 + lysA-1 DVU1647 0.61 +0.1 1,726,774 + lysA-1 DVU1647 0.62 -0.1
Or see this region's nucleotide sequence