Experiment: MoLS4 without Choline Chloride with Casaminos (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nspC and DORF27889 are separated by 41 nucleotides DORF27889 and DVU0420 are separated by 51 nucleotides DVU0420 and DVU0421 are separated by 10 nucleotides
DVU0419: nspC - carboxynorspermidine decarboxylase (TIGR), at 472,353 to 473,525
nspC
DORF27889: DORF27889 - no description, at 473,567 to 473,728
DORF27889
DVU0420: DVU0420 - hypothetical protein (TIGR), at 473,780 to 473,956
DVU0420
DVU0421: DVU0421 - agmatinase, putative (TIGR), at 473,967 to 474,839
DVU0421
Position (kb) 473
474 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 472.603 kb on - strand, within nspC at 472.638 kb on + strand, within nspC at 472.712 kb on + strand, within nspC at 472.791 kb on + strand, within nspC at 472.897 kb on + strand, within nspC at 472.905 kb on - strand, within nspC at 472.959 kb on + strand, within nspC at 473.043 kb on - strand, within nspC at 473.056 kb on - strand, within nspC at 473.064 kb on - strand, within nspC at 473.093 kb on - strand, within nspC at 473.098 kb on - strand, within nspC at 473.138 kb on + strand, within nspC at 473.160 kb on + strand, within nspC at 473.195 kb on - strand, within nspC at 473.207 kb on + strand, within nspC at 473.262 kb on - strand, within nspC at 473.317 kb on + strand, within nspC at 473.450 kb on + strand at 473.453 kb on - strand at 473.480 kb on - strand at 473.526 kb on - strand at 473.527 kb on - strand at 473.527 kb on - strand at 473.633 kb on - strand, within DORF27889 at 473.662 kb on + strand, within DORF27889 at 473.694 kb on + strand, within DORF27889 at 473.708 kb on - strand, within DORF27889 at 473.739 kb on + strand at 473.794 kb on + strand at 473.833 kb on + strand, within DVU0420 at 473.884 kb on + strand, within DVU0420 at 473.960 kb on - strand at 474.034 kb on + strand at 474.035 kb on + strand at 474.118 kb on + strand, within DVU0421 at 474.126 kb on - strand, within DVU0421 at 474.144 kb on + strand, within DVU0421 at 474.186 kb on + strand, within DVU0421 at 474.213 kb on - strand, within DVU0421 at 474.271 kb on - strand, within DVU0421 at 474.294 kb on + strand, within DVU0421 at 474.385 kb on + strand, within DVU0421 at 474.413 kb on - strand, within DVU0421 at 474.422 kb on - strand, within DVU0421 at 474.489 kb on - strand, within DVU0421 at 474.514 kb on + strand, within DVU0421 at 474.619 kb on + strand, within DVU0421 at 474.627 kb on - strand, within DVU0421 at 474.627 kb on - strand, within DVU0421
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without Choline Chloride with Casaminos (vit. assay) 0.05% remove 472,603 - nspC DVU0419 0.21 -1.9 472,638 + nspC DVU0419 0.24 +0.4 472,712 + nspC DVU0419 0.31 -1.0 472,791 + nspC DVU0419 0.37 -0.4 472,897 + nspC DVU0419 0.46 -0.8 472,905 - nspC DVU0419 0.47 -3.3 472,959 + nspC DVU0419 0.52 -0.3 473,043 - nspC DVU0419 0.59 -0.8 473,056 - nspC DVU0419 0.60 -0.7 473,064 - nspC DVU0419 0.61 -0.6 473,093 - nspC DVU0419 0.63 -0.9 473,098 - nspC DVU0419 0.64 +0.8 473,138 + nspC DVU0419 0.67 -0.7 473,160 + nspC DVU0419 0.69 -1.7 473,195 - nspC DVU0419 0.72 -0.3 473,207 + nspC DVU0419 0.73 -1.0 473,262 - nspC DVU0419 0.77 +0.8 473,317 + nspC DVU0419 0.82 +0.8 473,450 + -1.0 473,453 - -1.6 473,480 - -0.9 473,526 - -2.1 473,527 - -0.2 473,527 - -0.3 473,633 - DORF27889 0.41 -0.3 473,662 + DORF27889 0.59 -0.1 473,694 + DORF27889 0.78 +0.2 473,708 - DORF27889 0.87 -2.2 473,739 + +1.4 473,794 + -0.2 473,833 + DVU0420 0.30 +0.1 473,884 + DVU0420 0.59 -1.0 473,960 - +0.1 474,034 + -1.3 474,035 + -1.0 474,118 + DVU0421 0.17 -1.0 474,126 - DVU0421 0.18 -1.5 474,144 + DVU0421 0.20 -0.8 474,186 + DVU0421 0.25 -0.4 474,213 - DVU0421 0.28 -0.7 474,271 - DVU0421 0.35 -1.2 474,294 + DVU0421 0.37 -2.0 474,385 + DVU0421 0.48 -1.1 474,413 - DVU0421 0.51 -1.3 474,422 - DVU0421 0.52 +0.5 474,489 - DVU0421 0.60 +1.4 474,514 + DVU0421 0.63 -0.9 474,619 + DVU0421 0.75 -1.6 474,627 - DVU0421 0.76 -0.2 474,627 - DVU0421 0.76 -1.0
Or see this region's nucleotide sequence