Experiment: Betaine (C)(N); with MOPS; with chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS04055 and PFLU_RS04060 are separated by 12 nucleotides PFLU_RS04060 and PFLU_RS04065 are separated by 20 nucleotides PFLU_RS04065 and PFLU_RS04070 are separated by 331 nucleotides
PFLU_RS04055: PFLU_RS04055 - ABC transporter permease, at 919,768 to 920,421
_RS04055
PFLU_RS04060: PFLU_RS04060 - glycine betaine ABC transporter substrate-binding protein, at 920,434 to 921,327
_RS04060
PFLU_RS04065: PFLU_RS04065 - ABC transporter permease, at 921,348 to 922,064
_RS04065
PFLU_RS04070: PFLU_RS04070 - peptide chain release factor 3, at 922,396 to 923,979
_RS04070
Position (kb) 921
922
923 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 920.435 kb on + strand at 920.435 kb on + strand at 920.435 kb on + strand at 920.435 kb on + strand at 920.435 kb on + strand at 920.435 kb on + strand at 920.435 kb on + strand at 920.435 kb on + strand at 920.435 kb on + strand at 920.436 kb on - strand at 920.436 kb on - strand at 920.436 kb on - strand at 920.436 kb on - strand at 920.591 kb on + strand, within PFLU_RS04060 at 920.591 kb on + strand, within PFLU_RS04060 at 920.591 kb on + strand, within PFLU_RS04060 at 920.591 kb on + strand, within PFLU_RS04060 at 920.591 kb on + strand, within PFLU_RS04060 at 920.591 kb on + strand, within PFLU_RS04060 at 920.591 kb on + strand, within PFLU_RS04060 at 920.591 kb on + strand, within PFLU_RS04060 at 920.592 kb on - strand, within PFLU_RS04060 at 920.592 kb on - strand, within PFLU_RS04060 at 920.592 kb on - strand, within PFLU_RS04060 at 920.592 kb on - strand, within PFLU_RS04060 at 920.592 kb on - strand, within PFLU_RS04060 at 920.621 kb on + strand, within PFLU_RS04060 at 920.622 kb on - strand, within PFLU_RS04060 at 920.633 kb on + strand, within PFLU_RS04060 at 920.633 kb on + strand, within PFLU_RS04060 at 920.633 kb on + strand, within PFLU_RS04060 at 920.633 kb on + strand, within PFLU_RS04060 at 920.633 kb on + strand, within PFLU_RS04060 at 920.633 kb on + strand, within PFLU_RS04060 at 920.633 kb on + strand, within PFLU_RS04060 at 920.634 kb on - strand, within PFLU_RS04060 at 920.634 kb on - strand, within PFLU_RS04060 at 920.634 kb on - strand, within PFLU_RS04060 at 920.634 kb on - strand, within PFLU_RS04060 at 920.634 kb on - strand, within PFLU_RS04060 at 920.634 kb on - strand, within PFLU_RS04060 at 920.634 kb on - strand, within PFLU_RS04060 at 920.634 kb on - strand, within PFLU_RS04060 at 920.693 kb on + strand, within PFLU_RS04060 at 920.693 kb on + strand, within PFLU_RS04060 at 920.693 kb on + strand, within PFLU_RS04060 at 920.694 kb on - strand, within PFLU_RS04060 at 920.694 kb on - strand, within PFLU_RS04060 at 920.722 kb on - strand, within PFLU_RS04060 at 920.736 kb on - strand, within PFLU_RS04060 at 920.736 kb on - strand, within PFLU_RS04060 at 920.736 kb on - strand, within PFLU_RS04060 at 920.789 kb on + strand, within PFLU_RS04060 at 920.790 kb on - strand, within PFLU_RS04060 at 920.790 kb on - strand, within PFLU_RS04060 at 920.790 kb on - strand, within PFLU_RS04060 at 920.904 kb on + strand, within PFLU_RS04060 at 920.905 kb on - strand, within PFLU_RS04060 at 920.905 kb on - strand, within PFLU_RS04060 at 920.905 kb on - strand, within PFLU_RS04060 at 920.905 kb on - strand, within PFLU_RS04060 at 920.905 kb on - strand, within PFLU_RS04060 at 920.905 kb on - strand, within PFLU_RS04060 at 920.954 kb on + strand, within PFLU_RS04060 at 920.954 kb on + strand, within PFLU_RS04060 at 920.954 kb on + strand, within PFLU_RS04060 at 920.954 kb on + strand, within PFLU_RS04060 at 920.954 kb on + strand, within PFLU_RS04060 at 920.954 kb on + strand, within PFLU_RS04060 at 920.954 kb on + strand, within PFLU_RS04060 at 920.954 kb on + strand, within PFLU_RS04060 at 920.954 kb on + strand, within PFLU_RS04060 at 920.955 kb on - strand, within PFLU_RS04060 at 920.955 kb on - strand, within PFLU_RS04060 at 920.955 kb on - strand, within PFLU_RS04060 at 920.955 kb on - strand, within PFLU_RS04060 at 920.955 kb on - strand, within PFLU_RS04060 at 920.955 kb on - strand, within PFLU_RS04060 at 920.955 kb on - strand, within PFLU_RS04060 at 921.027 kb on - strand, within PFLU_RS04060 at 921.027 kb on - strand, within PFLU_RS04060 at 921.027 kb on - strand, within PFLU_RS04060 at 921.065 kb on + strand, within PFLU_RS04060 at 921.065 kb on + strand, within PFLU_RS04060 at 921.065 kb on + strand, within PFLU_RS04060 at 921.066 kb on - strand, within PFLU_RS04060 at 921.066 kb on - strand, within PFLU_RS04060 at 921.066 kb on - strand, within PFLU_RS04060 at 921.066 kb on - strand, within PFLU_RS04060 at 921.089 kb on + strand, within PFLU_RS04060 at 921.090 kb on - strand, within PFLU_RS04060 at 921.090 kb on - strand, within PFLU_RS04060 at 921.173 kb on + strand, within PFLU_RS04060 at 921.174 kb on - strand, within PFLU_RS04060 at 921.174 kb on - strand, within PFLU_RS04060 at 921.191 kb on + strand, within PFLU_RS04060 at 921.191 kb on + strand, within PFLU_RS04060 at 921.192 kb on - strand, within PFLU_RS04060 at 921.192 kb on - strand, within PFLU_RS04060 at 921.307 kb on + strand at 921.308 kb on - strand at 921.349 kb on + strand at 921.349 kb on + strand at 921.349 kb on + strand at 921.349 kb on + strand at 921.350 kb on - strand at 921.615 kb on + strand, within PFLU_RS04065 at 921.616 kb on - strand, within PFLU_RS04065 at 921.616 kb on - strand, within PFLU_RS04065 at 921.616 kb on - strand, within PFLU_RS04065 at 921.687 kb on + strand, within PFLU_RS04065 at 921.688 kb on + strand, within PFLU_RS04065 at 921.688 kb on + strand, within PFLU_RS04065 at 921.688 kb on + strand, within PFLU_RS04065 at 921.688 kb on + strand, within PFLU_RS04065 at 921.688 kb on + strand, within PFLU_RS04065 at 921.688 kb on + strand, within PFLU_RS04065 at 921.688 kb on + strand, within PFLU_RS04065 at 921.688 kb on + strand, within PFLU_RS04065 at 921.688 kb on + strand, within PFLU_RS04065 at 921.688 kb on + strand, within PFLU_RS04065 at 921.688 kb on + strand, within PFLU_RS04065 at 921.688 kb on + strand, within PFLU_RS04065 at 921.688 kb on + strand, within PFLU_RS04065 at 921.688 kb on + strand, within PFLU_RS04065 at 921.688 kb on + strand, within PFLU_RS04065 at 921.689 kb on - strand, within PFLU_RS04065 at 921.689 kb on - strand, within PFLU_RS04065 at 921.689 kb on - strand, within PFLU_RS04065 at 921.689 kb on - strand, within PFLU_RS04065 at 921.689 kb on - strand, within PFLU_RS04065 at 921.689 kb on - strand, within PFLU_RS04065 at 921.689 kb on - strand, within PFLU_RS04065 at 921.689 kb on - strand, within PFLU_RS04065 at 921.704 kb on + strand, within PFLU_RS04065 at 921.705 kb on - strand, within PFLU_RS04065 at 921.807 kb on - strand, within PFLU_RS04065 at 921.915 kb on + strand, within PFLU_RS04065 at 921.915 kb on + strand, within PFLU_RS04065 at 921.915 kb on + strand, within PFLU_RS04065 at 921.915 kb on + strand, within PFLU_RS04065 at 921.915 kb on + strand, within PFLU_RS04065 at 921.916 kb on - strand, within PFLU_RS04065 at 921.916 kb on - strand, within PFLU_RS04065 at 921.943 kb on + strand, within PFLU_RS04065 at 921.943 kb on + strand, within PFLU_RS04065 at 921.943 kb on + strand, within PFLU_RS04065 at 921.943 kb on + strand, within PFLU_RS04065 at 921.965 kb on - strand, within PFLU_RS04065 at 922.035 kb on + strand at 922.035 kb on + strand at 922.036 kb on - strand at 922.036 kb on - strand at 922.213 kb on - strand at 922.226 kb on - strand at 922.250 kb on - strand at 922.270 kb on + strand at 922.271 kb on - strand at 922.271 kb on - strand at 922.271 kb on - strand at 922.271 kb on - strand at 922.303 kb on + strand at 922.304 kb on - strand at 922.304 kb on - strand at 922.354 kb on + strand at 922.355 kb on - strand at 922.355 kb on - strand at 922.355 kb on - strand at 922.443 kb on + strand at 922.443 kb on + strand at 922.444 kb on - strand at 922.590 kb on + strand, within PFLU_RS04070 at 922.599 kb on + strand, within PFLU_RS04070 at 922.600 kb on - strand, within PFLU_RS04070 at 922.687 kb on + strand, within PFLU_RS04070 at 922.688 kb on - strand, within PFLU_RS04070 at 922.936 kb on + strand, within PFLU_RS04070 at 922.936 kb on + strand, within PFLU_RS04070 at 922.936 kb on + strand, within PFLU_RS04070 at 922.937 kb on - strand, within PFLU_RS04070 at 922.937 kb on - strand, within PFLU_RS04070 at 922.937 kb on - strand, within PFLU_RS04070 at 922.937 kb on - strand, within PFLU_RS04070 at 922.954 kb on + strand, within PFLU_RS04070 at 922.966 kb on + strand, within PFLU_RS04070 at 922.967 kb on - strand, within PFLU_RS04070 at 922.967 kb on - strand, within PFLU_RS04070
Per-strain Table
Position Strand Gene LocusTag Fraction Betaine (C)(N); with MOPS; with chloride remove 920,435 + -2.1 920,435 + +2.3 920,435 + -1.3 920,435 + -0.0 920,435 + -0.5 920,435 + -1.7 920,435 + -1.9 920,435 + -1.7 920,435 + -0.8 920,436 - +0.8 920,436 - +1.8 920,436 - -1.4 920,436 - -0.4 920,591 + PFLU_RS04060 0.18 -1.4 920,591 + PFLU_RS04060 0.18 -2.0 920,591 + PFLU_RS04060 0.18 -3.6 920,591 + PFLU_RS04060 0.18 -1.9 920,591 + PFLU_RS04060 0.18 -2.0 920,591 + PFLU_RS04060 0.18 -1.8 920,591 + PFLU_RS04060 0.18 -1.7 920,591 + PFLU_RS04060 0.18 -1.2 920,592 - PFLU_RS04060 0.18 -2.1 920,592 - PFLU_RS04060 0.18 -1.0 920,592 - PFLU_RS04060 0.18 -2.1 920,592 - PFLU_RS04060 0.18 -1.8 920,592 - PFLU_RS04060 0.18 -1.6 920,621 + PFLU_RS04060 0.21 -2.7 920,622 - PFLU_RS04060 0.21 -2.8 920,633 + PFLU_RS04060 0.22 -2.3 920,633 + PFLU_RS04060 0.22 -2.2 920,633 + PFLU_RS04060 0.22 -0.2 920,633 + PFLU_RS04060 0.22 -3.0 920,633 + PFLU_RS04060 0.22 -1.7 920,633 + PFLU_RS04060 0.22 -1.9 920,633 + PFLU_RS04060 0.22 -2.8 920,634 - PFLU_RS04060 0.22 -0.6 920,634 - PFLU_RS04060 0.22 -2.4 920,634 - PFLU_RS04060 0.22 -0.8 920,634 - PFLU_RS04060 0.22 -0.0 920,634 - PFLU_RS04060 0.22 -2.1 920,634 - PFLU_RS04060 0.22 -3.4 920,634 - PFLU_RS04060 0.22 -2.3 920,634 - PFLU_RS04060 0.22 +1.3 920,693 + PFLU_RS04060 0.29 -1.8 920,693 + PFLU_RS04060 0.29 -1.8 920,693 + PFLU_RS04060 0.29 -3.5 920,694 - PFLU_RS04060 0.29 -2.8 920,694 - PFLU_RS04060 0.29 -2.2 920,722 - PFLU_RS04060 0.32 -2.0 920,736 - PFLU_RS04060 0.34 +0.4 920,736 - PFLU_RS04060 0.34 -0.7 920,736 - PFLU_RS04060 0.34 +0.7 920,789 + PFLU_RS04060 0.40 -0.7 920,790 - PFLU_RS04060 0.40 -3.9 920,790 - PFLU_RS04060 0.40 +0.7 920,790 - PFLU_RS04060 0.40 -1.0 920,904 + PFLU_RS04060 0.53 -1.7 920,905 - PFLU_RS04060 0.53 -2.4 920,905 - PFLU_RS04060 0.53 +0.4 920,905 - PFLU_RS04060 0.53 -2.9 920,905 - PFLU_RS04060 0.53 -2.7 920,905 - PFLU_RS04060 0.53 -1.8 920,905 - PFLU_RS04060 0.53 -0.7 920,954 + PFLU_RS04060 0.58 -1.8 920,954 + PFLU_RS04060 0.58 -3.0 920,954 + PFLU_RS04060 0.58 -2.4 920,954 + PFLU_RS04060 0.58 +0.7 920,954 + PFLU_RS04060 0.58 -3.0 920,954 + PFLU_RS04060 0.58 -0.5 920,954 + PFLU_RS04060 0.58 -2.8 920,954 + PFLU_RS04060 0.58 -0.5 920,954 + PFLU_RS04060 0.58 -2.1 920,955 - PFLU_RS04060 0.58 -2.4 920,955 - PFLU_RS04060 0.58 -1.3 920,955 - PFLU_RS04060 0.58 -2.8 920,955 - PFLU_RS04060 0.58 -3.5 920,955 - PFLU_RS04060 0.58 +4.6 920,955 - PFLU_RS04060 0.58 -1.5 920,955 - PFLU_RS04060 0.58 -3.0 921,027 - PFLU_RS04060 0.66 -3.4 921,027 - PFLU_RS04060 0.66 -1.0 921,027 - PFLU_RS04060 0.66 -0.1 921,065 + PFLU_RS04060 0.71 -1.5 921,065 + PFLU_RS04060 0.71 -0.0 921,065 + PFLU_RS04060 0.71 -2.4 921,066 - PFLU_RS04060 0.71 +1.4 921,066 - PFLU_RS04060 0.71 -1.9 921,066 - PFLU_RS04060 0.71 -1.0 921,066 - PFLU_RS04060 0.71 -2.8 921,089 + PFLU_RS04060 0.73 -2.3 921,090 - PFLU_RS04060 0.73 -2.8 921,090 - PFLU_RS04060 0.73 -1.8 921,173 + PFLU_RS04060 0.83 -2.3 921,174 - PFLU_RS04060 0.83 -1.1 921,174 - PFLU_RS04060 0.83 -1.8 921,191 + PFLU_RS04060 0.85 -2.1 921,191 + PFLU_RS04060 0.85 -2.8 921,192 - PFLU_RS04060 0.85 -0.0 921,192 - PFLU_RS04060 0.85 -3.0 921,307 + -1.5 921,308 - -0.3 921,349 + -0.4 921,349 + -1.7 921,349 + -1.3 921,349 + -0.7 921,350 - -1.1 921,615 + PFLU_RS04065 0.37 -1.7 921,616 - PFLU_RS04065 0.37 -1.6 921,616 - PFLU_RS04065 0.37 -0.5 921,616 - PFLU_RS04065 0.37 -1.3 921,687 + PFLU_RS04065 0.47 -1.9 921,688 + PFLU_RS04065 0.47 -1.5 921,688 + PFLU_RS04065 0.47 -1.6 921,688 + PFLU_RS04065 0.47 -2.3 921,688 + PFLU_RS04065 0.47 -1.9 921,688 + PFLU_RS04065 0.47 -1.1 921,688 + PFLU_RS04065 0.47 -0.3 921,688 + PFLU_RS04065 0.47 -2.2 921,688 + PFLU_RS04065 0.47 -2.6 921,688 + PFLU_RS04065 0.47 -0.9 921,688 + PFLU_RS04065 0.47 -1.8 921,688 + PFLU_RS04065 0.47 -0.0 921,688 + PFLU_RS04065 0.47 -0.0 921,688 + PFLU_RS04065 0.47 -0.9 921,688 + PFLU_RS04065 0.47 -1.0 921,688 + PFLU_RS04065 0.47 -3.0 921,689 - PFLU_RS04065 0.48 -1.1 921,689 - PFLU_RS04065 0.48 -1.5 921,689 - PFLU_RS04065 0.48 -2.1 921,689 - PFLU_RS04065 0.48 -2.9 921,689 - PFLU_RS04065 0.48 -2.7 921,689 - PFLU_RS04065 0.48 -2.1 921,689 - PFLU_RS04065 0.48 -1.1 921,689 - PFLU_RS04065 0.48 -1.6 921,704 + PFLU_RS04065 0.50 -2.0 921,705 - PFLU_RS04065 0.50 -2.0 921,807 - PFLU_RS04065 0.64 -2.8 921,915 + PFLU_RS04065 0.79 -1.3 921,915 + PFLU_RS04065 0.79 -2.8 921,915 + PFLU_RS04065 0.79 -2.7 921,915 + PFLU_RS04065 0.79 -2.4 921,915 + PFLU_RS04065 0.79 +0.3 921,916 - PFLU_RS04065 0.79 +2.3 921,916 - PFLU_RS04065 0.79 -0.7 921,943 + PFLU_RS04065 0.83 +0.3 921,943 + PFLU_RS04065 0.83 -0.5 921,943 + PFLU_RS04065 0.83 -1.1 921,943 + PFLU_RS04065 0.83 -1.7 921,965 - PFLU_RS04065 0.86 -1.8 922,035 + -0.8 922,035 + -1.3 922,036 - -3.2 922,036 - -1.0 922,213 - +1.0 922,226 - +2.5 922,250 - -1.4 922,270 + -0.9 922,271 - -2.2 922,271 - +1.2 922,271 - +0.6 922,271 - -0.3 922,303 + -0.0 922,304 - +2.5 922,304 - -1.7 922,354 + +2.0 922,355 - +2.3 922,355 - +1.6 922,355 - -0.0 922,443 + +1.3 922,443 + +2.0 922,444 - +0.3 922,590 + PFLU_RS04070 0.12 +2.3 922,599 + PFLU_RS04070 0.13 +2.3 922,600 - PFLU_RS04070 0.13 +1.3 922,687 + PFLU_RS04070 0.18 -0.0 922,688 - PFLU_RS04070 0.18 +0.7 922,936 + PFLU_RS04070 0.34 -0.0 922,936 + PFLU_RS04070 0.34 +1.3 922,936 + PFLU_RS04070 0.34 +2.3 922,937 - PFLU_RS04070 0.34 +0.7 922,937 - PFLU_RS04070 0.34 +1.3 922,937 - PFLU_RS04070 0.34 +1.3 922,937 - PFLU_RS04070 0.34 +0.7 922,954 + PFLU_RS04070 0.35 +1.3 922,966 + PFLU_RS04070 0.36 +2.3 922,967 - PFLU_RS04070 0.36 +2.3 922,967 - PFLU_RS04070 0.36 +2.3
Or see this region's nucleotide sequence