Experiment: Betaine (C)(N); with MOPS; with chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS03385 and PFLU_RS03390 are separated by 187 nucleotides PFLU_RS03390 and PFLU_RS03395 overlap by 8 nucleotides PFLU_RS03395 and PFLU_RS03400 are separated by 121 nucleotides PFLU_RS03400 and PFLU_RS03405 are separated by 138 nucleotides
PFLU_RS03385: PFLU_RS03385 - hypothetical protein, at 781,179 to 782,456
_RS03385
PFLU_RS03390: PFLU_RS03390 - hypothetical protein, at 782,644 to 783,390
_RS03390
PFLU_RS03395: PFLU_RS03395 - ribosomal-protein-alanine N-acetyltransferase, at 783,383 to 783,835
_RS03395
PFLU_RS03400: PFLU_RS03400 - hypothetical protein, at 783,957 to 784,361
_RS03400
PFLU_RS03405: PFLU_RS03405 - carbonate dehydratase, at 784,500 to 785,144
_RS03405
Position (kb) 783
784 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 782.481 kb on + strand at 782.481 kb on + strand at 782.481 kb on + strand at 782.482 kb on - strand at 782.496 kb on + strand at 782.496 kb on + strand at 782.496 kb on + strand at 782.497 kb on - strand at 782.497 kb on - strand at 782.497 kb on - strand at 782.518 kb on - strand at 782.565 kb on + strand at 782.581 kb on + strand at 782.581 kb on + strand at 782.581 kb on + strand at 782.581 kb on + strand at 782.581 kb on + strand at 782.581 kb on + strand at 782.581 kb on + strand at 782.581 kb on + strand at 782.581 kb on + strand at 782.581 kb on + strand at 782.581 kb on + strand at 782.585 kb on + strand at 782.585 kb on + strand at 783.396 kb on + strand at 783.397 kb on - strand at 783.449 kb on + strand, within PFLU_RS03395 at 783.506 kb on + strand, within PFLU_RS03395 at 783.506 kb on + strand, within PFLU_RS03395 at 783.506 kb on + strand, within PFLU_RS03395 at 783.507 kb on - strand, within PFLU_RS03395 at 783.507 kb on - strand, within PFLU_RS03395 at 783.666 kb on - strand, within PFLU_RS03395 at 783.666 kb on - strand, within PFLU_RS03395 at 783.666 kb on - strand, within PFLU_RS03395 at 783.731 kb on + strand, within PFLU_RS03395 at 783.731 kb on + strand, within PFLU_RS03395 at 783.731 kb on + strand, within PFLU_RS03395 at 783.731 kb on + strand, within PFLU_RS03395 at 783.731 kb on + strand, within PFLU_RS03395 at 783.732 kb on - strand, within PFLU_RS03395 at 783.732 kb on - strand, within PFLU_RS03395 at 783.732 kb on - strand, within PFLU_RS03395 at 783.732 kb on - strand, within PFLU_RS03395 at 783.732 kb on - strand, within PFLU_RS03395 at 783.740 kb on + strand, within PFLU_RS03395 at 783.741 kb on - strand, within PFLU_RS03395 at 783.773 kb on + strand, within PFLU_RS03395 at 783.773 kb on + strand, within PFLU_RS03395 at 783.773 kb on + strand, within PFLU_RS03395 at 783.774 kb on - strand, within PFLU_RS03395 at 783.774 kb on - strand, within PFLU_RS03395 at 783.774 kb on - strand, within PFLU_RS03395 at 783.774 kb on - strand, within PFLU_RS03395 at 783.774 kb on - strand, within PFLU_RS03395 at 783.774 kb on - strand, within PFLU_RS03395 at 783.776 kb on + strand, within PFLU_RS03395 at 783.776 kb on + strand, within PFLU_RS03395 at 783.777 kb on - strand, within PFLU_RS03395 at 783.833 kb on + strand at 783.833 kb on + strand at 783.833 kb on + strand at 783.833 kb on + strand at 783.834 kb on - strand at 783.834 kb on - strand at 783.834 kb on - strand at 783.834 kb on - strand at 783.834 kb on - strand at 783.953 kb on + strand at 783.953 kb on + strand at 783.953 kb on + strand at 783.953 kb on + strand at 783.953 kb on + strand at 783.954 kb on - strand at 783.954 kb on - strand at 784.014 kb on - strand, within PFLU_RS03400 at 784.262 kb on + strand, within PFLU_RS03400 at 784.263 kb on - strand, within PFLU_RS03400 at 784.263 kb on - strand, within PFLU_RS03400 at 784.263 kb on - strand, within PFLU_RS03400 at 784.332 kb on - strand at 784.339 kb on + strand at 784.374 kb on + strand at 784.374 kb on + strand at 784.374 kb on + strand at 784.374 kb on + strand at 784.374 kb on + strand at 784.374 kb on + strand at 784.374 kb on + strand at 784.375 kb on - strand at 784.375 kb on - strand at 784.375 kb on - strand at 784.375 kb on - strand at 784.375 kb on - strand at 784.386 kb on + strand at 784.386 kb on + strand at 784.386 kb on + strand at 784.386 kb on + strand at 784.386 kb on + strand at 784.386 kb on + strand at 784.386 kb on + strand at 784.386 kb on + strand at 784.386 kb on + strand at 784.386 kb on + strand at 784.386 kb on + strand at 784.386 kb on + strand at 784.386 kb on + strand at 784.386 kb on + strand at 784.386 kb on + strand at 784.386 kb on + strand at 784.386 kb on + strand at 784.386 kb on + strand at 784.386 kb on + strand at 784.386 kb on + strand at 784.386 kb on + strand at 784.386 kb on + strand at 784.387 kb on - strand at 784.387 kb on - strand at 784.387 kb on - strand at 784.387 kb on - strand at 784.387 kb on - strand at 784.387 kb on - strand at 784.387 kb on - strand at 784.387 kb on - strand at 784.388 kb on + strand at 784.388 kb on + strand at 784.388 kb on + strand at 784.388 kb on + strand at 784.388 kb on + strand at 784.388 kb on + strand at 784.388 kb on + strand at 784.388 kb on + strand at 784.388 kb on + strand at 784.388 kb on + strand at 784.388 kb on + strand at 784.388 kb on + strand at 784.388 kb on + strand at 784.388 kb on + strand at 784.388 kb on + strand at 784.388 kb on + strand at 784.388 kb on + strand at 784.388 kb on + strand at 784.388 kb on + strand at 784.388 kb on + strand at 784.388 kb on + strand at 784.388 kb on + strand at 784.388 kb on + strand at 784.388 kb on + strand at 784.389 kb on - strand at 784.389 kb on - strand at 784.389 kb on - strand at 784.389 kb on - strand at 784.389 kb on - strand at 784.389 kb on - strand at 784.389 kb on - strand at 784.389 kb on - strand at 784.389 kb on - strand at 784.389 kb on - strand at 784.389 kb on - strand at 784.389 kb on - strand at 784.389 kb on - strand at 784.389 kb on - strand at 784.389 kb on - strand at 784.389 kb on - strand at 784.389 kb on - strand at 784.389 kb on - strand at 784.389 kb on - strand at 784.426 kb on + strand at 784.426 kb on + strand at 784.426 kb on + strand at 784.426 kb on + strand at 784.426 kb on + strand at 784.427 kb on - strand at 784.427 kb on - strand at 784.427 kb on - strand at 784.510 kb on + strand at 784.510 kb on + strand at 784.510 kb on + strand at 784.510 kb on + strand at 784.510 kb on + strand at 784.511 kb on - strand at 784.511 kb on - strand at 784.511 kb on - strand at 784.526 kb on + strand at 784.527 kb on - strand at 784.609 kb on - strand, within PFLU_RS03405 at 784.609 kb on - strand, within PFLU_RS03405 at 784.609 kb on - strand, within PFLU_RS03405 at 784.691 kb on + strand, within PFLU_RS03405 at 784.691 kb on + strand, within PFLU_RS03405 at 784.691 kb on + strand, within PFLU_RS03405 at 784.747 kb on - strand, within PFLU_RS03405 at 784.794 kb on + strand, within PFLU_RS03405 at 784.811 kb on - strand, within PFLU_RS03405
Per-strain Table
Position Strand Gene LocusTag Fraction Betaine (C)(N); with MOPS; with chloride remove 782,481 + +2.0 782,481 + +1.0 782,481 + +1.8 782,482 - +1.7 782,496 + +2.9 782,496 + +2.3 782,496 + +0.0 782,497 - -1.6 782,497 - -0.8 782,497 - -0.3 782,518 - +1.9 782,565 + -0.6 782,581 + +1.6 782,581 + -0.7 782,581 + -1.4 782,581 + -1.1 782,581 + -0.4 782,581 + -1.6 782,581 + -0.2 782,581 + -0.0 782,581 + -0.4 782,581 + +0.1 782,581 + +0.4 782,585 + +1.0 782,585 + +2.0 783,396 + +1.8 783,397 - +0.4 783,449 + PFLU_RS03395 0.15 -1.8 783,506 + PFLU_RS03395 0.27 -2.2 783,506 + PFLU_RS03395 0.27 -1.4 783,506 + PFLU_RS03395 0.27 -0.4 783,507 - PFLU_RS03395 0.27 -0.5 783,507 - PFLU_RS03395 0.27 +0.9 783,666 - PFLU_RS03395 0.62 -1.8 783,666 - PFLU_RS03395 0.62 -2.4 783,666 - PFLU_RS03395 0.62 -2.7 783,731 + PFLU_RS03395 0.77 -1.5 783,731 + PFLU_RS03395 0.77 -0.4 783,731 + PFLU_RS03395 0.77 +0.3 783,731 + PFLU_RS03395 0.77 +0.3 783,731 + PFLU_RS03395 0.77 +0.7 783,732 - PFLU_RS03395 0.77 -1.0 783,732 - PFLU_RS03395 0.77 +1.8 783,732 - PFLU_RS03395 0.77 -1.3 783,732 - PFLU_RS03395 0.77 -0.3 783,732 - PFLU_RS03395 0.77 -1.6 783,740 + PFLU_RS03395 0.79 -1.9 783,741 - PFLU_RS03395 0.79 +0.0 783,773 + PFLU_RS03395 0.86 +0.6 783,773 + PFLU_RS03395 0.86 -2.2 783,773 + PFLU_RS03395 0.86 -0.2 783,774 - PFLU_RS03395 0.86 -1.2 783,774 - PFLU_RS03395 0.86 +0.0 783,774 - PFLU_RS03395 0.86 -0.8 783,774 - PFLU_RS03395 0.86 -0.5 783,774 - PFLU_RS03395 0.86 -1.3 783,774 - PFLU_RS03395 0.86 -1.0 783,776 + PFLU_RS03395 0.87 -1.8 783,776 + PFLU_RS03395 0.87 -0.4 783,777 - PFLU_RS03395 0.87 -1.3 783,833 + +3.0 783,833 + -0.7 783,833 + +1.2 783,833 + +1.4 783,834 - -0.1 783,834 - +0.0 783,834 - +1.0 783,834 - -1.3 783,834 - +0.4 783,953 + +0.7 783,953 + +1.6 783,953 + -1.9 783,953 + -0.8 783,953 + -0.5 783,954 - +3.0 783,954 - +0.1 784,014 - PFLU_RS03400 0.14 -1.8 784,262 + PFLU_RS03400 0.75 +0.5 784,263 - PFLU_RS03400 0.76 -0.8 784,263 - PFLU_RS03400 0.76 -0.8 784,263 - PFLU_RS03400 0.76 -0.9 784,332 - -1.6 784,339 + +0.3 784,374 + +0.0 784,374 + +0.3 784,374 + +0.5 784,374 + -0.8 784,374 + -0.8 784,374 + -1.1 784,374 + +1.0 784,375 - -1.2 784,375 - -0.8 784,375 - -1.4 784,375 - -1.0 784,375 - -2.6 784,386 + +1.6 784,386 + -2.8 784,386 + -0.4 784,386 + +1.9 784,386 + +1.0 784,386 + +0.3 784,386 + +0.4 784,386 + -0.6 784,386 + +1.3 784,386 + -1.5 784,386 + -2.3 784,386 + +1.3 784,386 + +1.5 784,386 + -0.6 784,386 + +0.0 784,386 + -1.0 784,386 + -0.8 784,386 + -3.0 784,386 + -2.9 784,386 + +0.7 784,386 + -0.7 784,386 + +1.5 784,387 - -0.8 784,387 - +1.7 784,387 - -0.2 784,387 - +0.6 784,387 - +0.5 784,387 - -1.0 784,387 - +0.3 784,387 - -1.3 784,388 + -0.7 784,388 + +1.2 784,388 + +0.0 784,388 + +1.1 784,388 + +2.3 784,388 + -2.0 784,388 + +0.7 784,388 + +1.3 784,388 + +0.0 784,388 + +1.1 784,388 + +2.0 784,388 + +4.2 784,388 + +1.0 784,388 + +1.3 784,388 + +2.1 784,388 + +0.4 784,388 + -1.0 784,388 + -1.1 784,388 + -0.2 784,388 + -2.1 784,388 + -0.6 784,388 + -0.1 784,388 + +0.9 784,388 + -0.8 784,389 - +0.7 784,389 - -1.5 784,389 - +0.7 784,389 - -0.5 784,389 - -1.4 784,389 - +0.2 784,389 - -0.7 784,389 - +0.2 784,389 - -1.4 784,389 - +1.0 784,389 - -1.8 784,389 - +0.3 784,389 - -0.6 784,389 - +1.7 784,389 - +0.4 784,389 - -0.2 784,389 - -0.6 784,389 - -1.6 784,389 - -1.7 784,426 + +1.4 784,426 + +0.7 784,426 + +0.9 784,426 + +3.2 784,426 + +0.4 784,427 - -0.1 784,427 - +0.0 784,427 - -2.1 784,510 + +0.0 784,510 + +0.4 784,510 + -2.4 784,510 + +3.7 784,510 + -0.7 784,511 - -0.8 784,511 - -1.6 784,511 - +0.0 784,526 + -2.1 784,527 - +0.8 784,609 - PFLU_RS03405 0.17 -1.8 784,609 - PFLU_RS03405 0.17 +0.4 784,609 - PFLU_RS03405 0.17 -1.8 784,691 + PFLU_RS03405 0.30 -2.1 784,691 + PFLU_RS03405 0.30 +1.4 784,691 + PFLU_RS03405 0.30 +1.6 784,747 - PFLU_RS03405 0.38 +0.4 784,794 + PFLU_RS03405 0.46 -3.0 784,811 - PFLU_RS03405 0.48 -2.1
Or see this region's nucleotide sequence