Experiment: Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25690 and BWI76_RS25695 overlap by 47 nucleotides BWI76_RS25695 and BWI76_RS25700 are separated by 58 nucleotides BWI76_RS25700 and BWI76_RS25705 are separated by 2 nucleotides BWI76_RS25705 and BWI76_RS25710 are separated by 107 nucleotides BWI76_RS25710 and BWI76_RS25715 are separated by 40 nucleotides
BWI76_RS25690: BWI76_RS25690 - large-conductance mechanosensitive channel, at 5,334,664 to 5,335,077
_RS25690
BWI76_RS25695: BWI76_RS25695 - ribosome alternative rescue factor ArfA, at 5,335,031 to 5,335,291
_RS25695
BWI76_RS25700: BWI76_RS25700 - heavy metal-responsive transcriptional regulator, at 5,335,350 to 5,335,775
_RS25700
BWI76_RS25705: BWI76_RS25705 - hypothetical protein, at 5,335,778 to 5,336,146
_RS25705
BWI76_RS25710: BWI76_RS25710 - 50S ribosomal protein L17, at 5,336,254 to 5,336,640
_RS25710
BWI76_RS25715: BWI76_RS25715 - DNA-directed RNA polymerase subunit alpha, at 5,336,681 to 5,337,670
_RS25715
Position (kb) 5335
5336
5337 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 5334.793 kb on + strand, within BWI76_RS25690 at 5334.799 kb on + strand, within BWI76_RS25690 at 5334.801 kb on - strand, within BWI76_RS25690 at 5334.829 kb on - strand, within BWI76_RS25690 at 5334.851 kb on - strand, within BWI76_RS25690 at 5334.852 kb on + strand, within BWI76_RS25690 at 5334.854 kb on + strand, within BWI76_RS25690 at 5334.874 kb on + strand, within BWI76_RS25690 at 5334.874 kb on + strand, within BWI76_RS25690 at 5334.874 kb on - strand, within BWI76_RS25690 at 5334.882 kb on + strand, within BWI76_RS25690 at 5334.997 kb on + strand, within BWI76_RS25690 at 5335.000 kb on + strand, within BWI76_RS25690 at 5335.001 kb on + strand, within BWI76_RS25690 at 5335.006 kb on + strand, within BWI76_RS25690 at 5335.019 kb on + strand, within BWI76_RS25690 at 5335.032 kb on - strand at 5335.032 kb on - strand at 5335.032 kb on - strand at 5335.127 kb on + strand, within BWI76_RS25695 at 5335.136 kb on - strand, within BWI76_RS25695 at 5335.143 kb on - strand, within BWI76_RS25695 at 5335.189 kb on - strand, within BWI76_RS25695 at 5335.189 kb on - strand, within BWI76_RS25695 at 5335.209 kb on - strand, within BWI76_RS25695 at 5335.226 kb on + strand, within BWI76_RS25695 at 5335.248 kb on - strand, within BWI76_RS25695 at 5335.269 kb on + strand at 5335.277 kb on - strand at 5335.287 kb on + strand at 5335.305 kb on - strand at 5335.326 kb on + strand at 5335.326 kb on + strand at 5335.326 kb on + strand at 5335.326 kb on + strand at 5335.334 kb on - strand at 5335.416 kb on + strand, within BWI76_RS25700 at 5335.424 kb on - strand, within BWI76_RS25700 at 5335.424 kb on - strand, within BWI76_RS25700 at 5335.424 kb on - strand, within BWI76_RS25700 at 5335.512 kb on + strand, within BWI76_RS25700 at 5335.520 kb on - strand, within BWI76_RS25700 at 5335.525 kb on - strand, within BWI76_RS25700 at 5335.600 kb on + strand, within BWI76_RS25700 at 5335.691 kb on + strand, within BWI76_RS25700 at 5335.858 kb on + strand, within BWI76_RS25705 at 5335.891 kb on + strand, within BWI76_RS25705 at 5335.913 kb on + strand, within BWI76_RS25705 at 5336.028 kb on - strand, within BWI76_RS25705 at 5336.099 kb on - strand, within BWI76_RS25705
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 5,334,793 + BWI76_RS25690 0.31 -1.0 5,334,799 + BWI76_RS25690 0.33 -1.6 5,334,801 - BWI76_RS25690 0.33 +1.4 5,334,829 - BWI76_RS25690 0.40 +0.2 5,334,851 - BWI76_RS25690 0.45 +1.0 5,334,852 + BWI76_RS25690 0.45 -0.7 5,334,854 + BWI76_RS25690 0.46 -1.4 5,334,874 + BWI76_RS25690 0.51 -1.5 5,334,874 + BWI76_RS25690 0.51 +0.3 5,334,874 - BWI76_RS25690 0.51 -0.5 5,334,882 + BWI76_RS25690 0.53 -1.6 5,334,997 + BWI76_RS25690 0.80 +0.1 5,335,000 + BWI76_RS25690 0.81 -0.1 5,335,001 + BWI76_RS25690 0.81 +0.6 5,335,006 + BWI76_RS25690 0.83 -0.8 5,335,019 + BWI76_RS25690 0.86 -1.2 5,335,032 - +0.7 5,335,032 - -3.5 5,335,032 - +0.8 5,335,127 + BWI76_RS25695 0.37 -0.2 5,335,136 - BWI76_RS25695 0.40 -1.6 5,335,143 - BWI76_RS25695 0.43 +0.5 5,335,189 - BWI76_RS25695 0.61 +0.9 5,335,189 - BWI76_RS25695 0.61 +1.8 5,335,209 - BWI76_RS25695 0.68 +0.8 5,335,226 + BWI76_RS25695 0.75 -1.8 5,335,248 - BWI76_RS25695 0.83 -0.1 5,335,269 + +1.1 5,335,277 - +1.0 5,335,287 + -0.6 5,335,305 - +0.0 5,335,326 + +0.1 5,335,326 + +1.0 5,335,326 + +0.3 5,335,326 + +0.5 5,335,334 - -1.2 5,335,416 + BWI76_RS25700 0.15 -3.1 5,335,424 - BWI76_RS25700 0.17 -3.6 5,335,424 - BWI76_RS25700 0.17 -3.9 5,335,424 - BWI76_RS25700 0.17 -3.3 5,335,512 + BWI76_RS25700 0.38 -3.1 5,335,520 - BWI76_RS25700 0.40 -4.6 5,335,525 - BWI76_RS25700 0.41 -4.8 5,335,600 + BWI76_RS25700 0.59 +1.0 5,335,691 + BWI76_RS25700 0.80 -3.6 5,335,858 + BWI76_RS25705 0.22 -0.6 5,335,891 + BWI76_RS25705 0.31 -2.0 5,335,913 + BWI76_RS25705 0.37 -2.2 5,336,028 - BWI76_RS25705 0.68 -1.4 5,336,099 - BWI76_RS25705 0.87 +0.2
Or see this region's nucleotide sequence