Experiment: Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21340 and BWI76_RS21345 overlap by 4 nucleotides BWI76_RS21345 and BWI76_RS21350 overlap by 1 nucleotides BWI76_RS21350 and BWI76_RS21355 are separated by 32 nucleotides
BWI76_RS21340: BWI76_RS21340 - SoxR reducing system protein RseC, at 4,467,978 to 4,468,457
_RS21340
BWI76_RS21345: BWI76_RS21345 - sigma-E factor regulatory protein RseB, at 4,468,454 to 4,469,410
_RS21345
BWI76_RS21350: BWI76_RS21350 - anti-RNA polymerase sigma factor SigE, at 4,469,410 to 4,470,060
_RS21350
BWI76_RS21355: BWI76_RS21355 - RNA polymerase sigma factor RpoE, at 4,470,093 to 4,470,668
_RS21355
Position (kb) 4469
4470
4471 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 4468.534 kb on - strand at 4468.867 kb on + strand, within BWI76_RS21345 at 4468.875 kb on - strand, within BWI76_RS21345 at 4469.057 kb on - strand, within BWI76_RS21345 at 4469.491 kb on - strand, within BWI76_RS21350 at 4469.752 kb on - strand, within BWI76_RS21350 at 4469.807 kb on + strand, within BWI76_RS21350 at 4470.071 kb on - strand at 4470.738 kb on - strand at 4470.757 kb on - strand at 4471.004 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 4,468,534 - -3.9 4,468,867 + BWI76_RS21345 0.43 -1.6 4,468,875 - BWI76_RS21345 0.44 +0.0 4,469,057 - BWI76_RS21345 0.63 -3.1 4,469,491 - BWI76_RS21350 0.12 -3.4 4,469,752 - BWI76_RS21350 0.53 -4.6 4,469,807 + BWI76_RS21350 0.61 -3.8 4,470,071 - -4.0 4,470,738 - -0.0 4,470,757 - -0.5 4,471,004 - +0.0
Or see this region's nucleotide sequence