Experiment: Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15970 and BWI76_RS15975 are separated by 176 nucleotides BWI76_RS15975 and BWI76_RS15980 are separated by 149 nucleotides BWI76_RS15980 and BWI76_RS15985 overlap by 20 nucleotides
BWI76_RS15970: BWI76_RS15970 - carboxypeptidase M32, at 3,313,078 to 3,314,565
_RS15970
BWI76_RS15975: BWI76_RS15975 - hypothetical protein, at 3,314,742 to 3,315,167
_RS15975
BWI76_RS15980: BWI76_RS15980 - lysine transporter LysE, at 3,315,317 to 3,315,579
_RS15980
BWI76_RS15985: BWI76_RS15985 - LysR family transcriptional regulator, at 3,315,560 to 3,315,784
_RS15985
Position (kb)
3314
3315
3316 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3314.303 kb on - strand, within BWI76_RS15970 at 3314.645 kb on + strand at 3314.730 kb on + strand at 3314.782 kb on - strand at 3314.982 kb on + strand, within BWI76_RS15975 at 3315.261 kb on - strand at 3315.433 kb on - strand, within BWI76_RS15980 at 3315.584 kb on + strand, within BWI76_RS15985 at 3315.602 kb on + strand, within BWI76_RS15985 at 3315.610 kb on - strand, within BWI76_RS15985 at 3315.746 kb on - strand, within BWI76_RS15985 at 3315.951 kb on + strand at 3315.968 kb on - strand at 3316.079 kb on - strand at 3316.079 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 3,314,303 - BWI76_RS15970 0.82 +0.2 3,314,645 + -1.6 3,314,730 + -1.1 3,314,782 - -3.3 3,314,982 + BWI76_RS15975 0.56 -2.6 3,315,261 - +0.1 3,315,433 - BWI76_RS15980 0.44 +0.5 3,315,584 + BWI76_RS15985 0.11 +0.1 3,315,602 + BWI76_RS15985 0.19 -0.8 3,315,610 - BWI76_RS15985 0.22 +0.3 3,315,746 - BWI76_RS15985 0.83 +0.1 3,315,951 + +1.0 3,315,968 - +0.4 3,316,079 - -0.6 3,316,079 - +0.9
Or see this region's nucleotide sequence