Experiment: Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11605 and BWI76_RS11610 are separated by 70 nucleotides BWI76_RS11610 and BWI76_RS11615 overlap by 45 nucleotides BWI76_RS11615 and BWI76_RS11620 are separated by 29 nucleotides BWI76_RS11620 and BWI76_RS11625 are separated by 86 nucleotides
BWI76_RS11605: BWI76_RS11605 - DNA topoisomerase III, at 2,442,026 to 2,443,981
_RS11605
BWI76_RS11610: BWI76_RS11610 - glutamate dehydrogenase, at 2,444,052 to 2,445,395
_RS11610
BWI76_RS11615: BWI76_RS11615 - hypothetical protein, at 2,445,351 to 2,445,548
_RS11615
BWI76_RS11620: BWI76_RS11620 - pyrimidine (deoxy)nucleoside triphosphate pyrophosphohydrolase, at 2,445,578 to 2,445,988
_RS11620
BWI76_RS11625: BWI76_RS11625 - membrane protein, at 2,446,075 to 2,446,692
_RS11625
Position (kb)
2444
2445
2446 Strain fitness (log2 ratio)
-2
-1
0
1 at 2444.153 kb on - strand at 2444.284 kb on + strand, within BWI76_RS11610 at 2444.439 kb on - strand, within BWI76_RS11610 at 2444.464 kb on - strand, within BWI76_RS11610 at 2445.215 kb on - strand, within BWI76_RS11610 at 2445.384 kb on + strand at 2446.039 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 2,444,153 - -0.4 2,444,284 + BWI76_RS11610 0.17 +0.1 2,444,439 - BWI76_RS11610 0.29 +1.1 2,444,464 - BWI76_RS11610 0.31 -1.6 2,445,215 - BWI76_RS11610 0.87 -2.4 2,445,384 + -2.5 2,446,039 - +0.5
Or see this region's nucleotide sequence