Experiment: Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08755 and BWI76_RS08760 are separated by 85 nucleotides BWI76_RS08760 and BWI76_RS08765 overlap by 4 nucleotides
BWI76_RS08755: BWI76_RS08755 - adenosylmethionine--8-amino-7-oxononanoate transaminase, at 1,845,038 to 1,846,327
_RS08755
BWI76_RS08760: BWI76_RS08760 - biotin synthase, at 1,846,413 to 1,847,453
_RS08760
BWI76_RS08765: BWI76_RS08765 - 8-amino-7-oxononanoate synthase, at 1,847,450 to 1,848,607
_RS08765
Position (kb) 1846
1847
1848 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1845.557 kb on + strand, within BWI76_RS08755 at 1845.732 kb on + strand, within BWI76_RS08755 at 1845.852 kb on - strand, within BWI76_RS08755 at 1845.936 kb on + strand, within BWI76_RS08755 at 1846.147 kb on + strand, within BWI76_RS08755 at 1846.183 kb on - strand, within BWI76_RS08755 at 1846.408 kb on + strand at 1846.536 kb on - strand, within BWI76_RS08760 at 1846.653 kb on + strand, within BWI76_RS08760 at 1846.747 kb on + strand, within BWI76_RS08760 at 1846.872 kb on + strand, within BWI76_RS08760 at 1846.937 kb on - strand, within BWI76_RS08760 at 1847.691 kb on + strand, within BWI76_RS08765 at 1847.826 kb on - strand, within BWI76_RS08765 at 1848.089 kb on + strand, within BWI76_RS08765
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 1,845,557 + BWI76_RS08755 0.40 -0.6 1,845,732 + BWI76_RS08755 0.54 +2.3 1,845,852 - BWI76_RS08755 0.63 -2.0 1,845,936 + BWI76_RS08755 0.70 -1.1 1,846,147 + BWI76_RS08755 0.86 -0.8 1,846,183 - BWI76_RS08755 0.89 -0.1 1,846,408 + -1.5 1,846,536 - BWI76_RS08760 0.12 -2.1 1,846,653 + BWI76_RS08760 0.23 -1.8 1,846,747 + BWI76_RS08760 0.32 -3.3 1,846,872 + BWI76_RS08760 0.44 +0.2 1,846,937 - BWI76_RS08760 0.50 -2.2 1,847,691 + BWI76_RS08765 0.21 -0.0 1,847,826 - BWI76_RS08765 0.32 +1.0 1,848,089 + BWI76_RS08765 0.55 -1.0
Or see this region's nucleotide sequence