Experiment: BHIS with Trimethoprim 15 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4394 and BT4395 are separated by 205 nucleotides BT4395 and BT4396 are separated by 94 nucleotides
BT4394: BT4394 - beta-hexosaminidase precursor (NCBI ptt file), at 5,786,139 to 5,787,779
BT4394
BT4395: BT4395 - hyaluronoglucosaminidase precursor (NCBI ptt file), at 5,787,985 to 5,790,198
BT4395
BT4396: BT4396 - transcriptional regulator (NCBI ptt file), at 5,790,293 to 5,791,180
BT4396
Position (kb)
5787
5788
5789
5790
5791 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 5786.986 kb on - strand, within BT4394 at 5787.027 kb on + strand, within BT4394 at 5787.042 kb on - strand, within BT4394 at 5787.097 kb on + strand, within BT4394 at 5787.098 kb on - strand, within BT4394 at 5787.285 kb on - strand, within BT4394 at 5787.363 kb on + strand, within BT4394 at 5787.364 kb on - strand, within BT4394 at 5787.602 kb on + strand, within BT4394 at 5787.623 kb on - strand at 5787.751 kb on - strand at 5787.800 kb on + strand at 5787.809 kb on + strand at 5787.832 kb on - strand at 5787.904 kb on - strand at 5787.914 kb on + strand at 5787.949 kb on - strand at 5787.951 kb on - strand at 5787.978 kb on - strand at 5788.004 kb on - strand at 5788.036 kb on - strand at 5788.161 kb on - strand at 5788.215 kb on + strand, within BT4395 at 5788.251 kb on + strand, within BT4395 at 5788.252 kb on - strand, within BT4395 at 5788.256 kb on - strand, within BT4395 at 5788.289 kb on - strand, within BT4395 at 5788.292 kb on - strand, within BT4395 at 5788.349 kb on - strand, within BT4395 at 5788.349 kb on - strand, within BT4395 at 5788.352 kb on - strand, within BT4395 at 5788.456 kb on + strand, within BT4395 at 5788.529 kb on - strand, within BT4395 at 5788.529 kb on - strand, within BT4395 at 5788.530 kb on + strand, within BT4395 at 5788.535 kb on - strand, within BT4395 at 5788.776 kb on + strand, within BT4395 at 5788.777 kb on - strand, within BT4395 at 5788.777 kb on - strand, within BT4395 at 5788.826 kb on - strand, within BT4395 at 5788.862 kb on + strand, within BT4395 at 5788.862 kb on + strand, within BT4395 at 5788.891 kb on + strand, within BT4395 at 5788.892 kb on - strand, within BT4395 at 5788.933 kb on - strand, within BT4395 at 5789.051 kb on - strand, within BT4395 at 5789.114 kb on - strand, within BT4395 at 5789.124 kb on + strand, within BT4395 at 5789.127 kb on + strand, within BT4395 at 5789.157 kb on - strand, within BT4395 at 5789.236 kb on + strand, within BT4395 at 5789.237 kb on - strand, within BT4395 at 5789.241 kb on + strand, within BT4395 at 5789.368 kb on - strand, within BT4395 at 5789.368 kb on - strand, within BT4395 at 5789.368 kb on - strand, within BT4395 at 5789.456 kb on - strand, within BT4395 at 5789.469 kb on + strand, within BT4395 at 5789.470 kb on - strand, within BT4395 at 5789.595 kb on - strand, within BT4395 at 5789.665 kb on + strand, within BT4395 at 5789.665 kb on + strand, within BT4395 at 5789.709 kb on - strand, within BT4395 at 5789.787 kb on + strand, within BT4395 at 5789.838 kb on + strand, within BT4395 at 5789.889 kb on - strand, within BT4395 at 5789.977 kb on - strand at 5790.009 kb on + strand at 5790.184 kb on - strand at 5790.206 kb on + strand at 5790.206 kb on + strand at 5790.344 kb on - strand at 5790.363 kb on + strand at 5790.371 kb on - strand at 5790.383 kb on + strand, within BT4396 at 5790.391 kb on - strand, within BT4396 at 5790.396 kb on - strand, within BT4396 at 5790.396 kb on - strand, within BT4396 at 5790.605 kb on + strand, within BT4396 at 5790.606 kb on - strand, within BT4396 at 5790.641 kb on - strand, within BT4396 at 5790.689 kb on - strand, within BT4396 at 5790.718 kb on + strand, within BT4396 at 5790.719 kb on - strand, within BT4396 at 5790.737 kb on - strand, within BT4396 at 5790.743 kb on + strand, within BT4396 at 5790.828 kb on + strand, within BT4396 at 5790.897 kb on + strand, within BT4396 at 5790.930 kb on + strand, within BT4396 at 5790.931 kb on - strand, within BT4396 at 5790.952 kb on - strand, within BT4396 at 5790.992 kb on - strand, within BT4396 at 5790.997 kb on - strand, within BT4396 at 5791.009 kb on - strand, within BT4396 at 5791.137 kb on - strand at 5791.175 kb on + strand at 5791.181 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 15 uM remove 5,786,986 - BT4394 0.52 -0.3 5,787,027 + BT4394 0.54 -0.5 5,787,042 - BT4394 0.55 -0.4 5,787,097 + BT4394 0.58 -0.8 5,787,098 - BT4394 0.58 -1.2 5,787,285 - BT4394 0.70 -1.6 5,787,363 + BT4394 0.75 -3.0 5,787,364 - BT4394 0.75 -0.3 5,787,602 + BT4394 0.89 -1.6 5,787,623 - -1.0 5,787,751 - -2.7 5,787,800 + -1.3 5,787,809 + -0.0 5,787,832 - -1.6 5,787,904 - -0.7 5,787,914 + -0.0 5,787,949 - -0.0 5,787,951 - -3.8 5,787,978 - -0.0 5,788,004 - -1.9 5,788,036 - -1.8 5,788,161 - -1.0 5,788,215 + BT4395 0.10 -2.3 5,788,251 + BT4395 0.12 +2.2 5,788,252 - BT4395 0.12 +1.2 5,788,256 - BT4395 0.12 +2.0 5,788,289 - BT4395 0.14 -0.0 5,788,292 - BT4395 0.14 -0.3 5,788,349 - BT4395 0.16 -0.6 5,788,349 - BT4395 0.16 -0.0 5,788,352 - BT4395 0.17 -1.0 5,788,456 + BT4395 0.21 -2.0 5,788,529 - BT4395 0.25 -1.0 5,788,529 - BT4395 0.25 -0.0 5,788,530 + BT4395 0.25 +0.3 5,788,535 - BT4395 0.25 -1.6 5,788,776 + BT4395 0.36 -2.6 5,788,777 - BT4395 0.36 +0.3 5,788,777 - BT4395 0.36 -1.4 5,788,826 - BT4395 0.38 -0.7 5,788,862 + BT4395 0.40 -0.8 5,788,862 + BT4395 0.40 -0.0 5,788,891 + BT4395 0.41 -1.2 5,788,892 - BT4395 0.41 -1.0 5,788,933 - BT4395 0.43 +0.7 5,789,051 - BT4395 0.48 -1.0 5,789,114 - BT4395 0.51 -0.7 5,789,124 + BT4395 0.51 -0.0 5,789,127 + BT4395 0.52 -2.0 5,789,157 - BT4395 0.53 -0.0 5,789,236 + BT4395 0.57 -1.0 5,789,237 - BT4395 0.57 -1.2 5,789,241 + BT4395 0.57 -2.3 5,789,368 - BT4395 0.62 -1.6 5,789,368 - BT4395 0.62 +1.0 5,789,368 - BT4395 0.62 -1.6 5,789,456 - BT4395 0.66 -1.6 5,789,469 + BT4395 0.67 +0.7 5,789,470 - BT4395 0.67 -0.0 5,789,595 - BT4395 0.73 -2.3 5,789,665 + BT4395 0.76 -0.0 5,789,665 + BT4395 0.76 +2.1 5,789,709 - BT4395 0.78 -1.3 5,789,787 + BT4395 0.81 -0.1 5,789,838 + BT4395 0.84 -1.0 5,789,889 - BT4395 0.86 +0.7 5,789,977 - -0.0 5,790,009 + +1.0 5,790,184 - -0.0 5,790,206 + -1.3 5,790,206 + -2.0 5,790,344 - +2.0 5,790,363 + -1.5 5,790,371 - -0.0 5,790,383 + BT4396 0.10 -2.0 5,790,391 - BT4396 0.11 -0.4 5,790,396 - BT4396 0.12 -0.0 5,790,396 - BT4396 0.12 -1.6 5,790,605 + BT4396 0.35 -1.3 5,790,606 - BT4396 0.35 -0.0 5,790,641 - BT4396 0.39 -1.8 5,790,689 - BT4396 0.45 +1.7 5,790,718 + BT4396 0.48 -0.9 5,790,719 - BT4396 0.48 -0.0 5,790,737 - BT4396 0.50 -2.0 5,790,743 + BT4396 0.51 -0.0 5,790,828 + BT4396 0.60 -1.6 5,790,897 + BT4396 0.68 -1.0 5,790,930 + BT4396 0.72 +0.8 5,790,931 - BT4396 0.72 -1.5 5,790,952 - BT4396 0.74 -2.5 5,790,992 - BT4396 0.79 -1.6 5,790,997 - BT4396 0.79 -0.8 5,791,009 - BT4396 0.81 -1.2 5,791,137 - -1.0 5,791,175 + +1.0 5,791,181 - +0.6
Or see this region's nucleotide sequence