Experiment: BHIS with Trimethoprim 15 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4273 and BT4274 are separated by 609 nucleotides BT4274 and BT4275 are separated by 110 nucleotides BT4275 and BT4276 are separated by 80 nucleotides
BT4273: BT4273 - conserved hypothetical protein (NCBI ptt file), at 5,631,593 to 5,633,152
BT4273
BT4274: BT4274 - hypothetical protein (NCBI ptt file), at 5,633,762 to 5,634,553
BT4274
BT4275: BT4275 - hypothetical protein (NCBI ptt file), at 5,634,664 to 5,635,422
BT4275
BT4276: BT4276 - conserved hypothetical protein, putative transposase (NCBI ptt file), at 5,635,503 to 5,635,874
BT4276
Position (kb)
5633
5634
5635 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5632.840 kb on + strand, within BT4273 at 5632.896 kb on - strand, within BT4273 at 5632.926 kb on + strand, within BT4273 at 5632.937 kb on - strand, within BT4273 at 5632.937 kb on - strand, within BT4273 at 5632.985 kb on + strand, within BT4273 at 5632.988 kb on + strand, within BT4273 at 5632.989 kb on - strand, within BT4273 at 5633.066 kb on - strand at 5633.084 kb on - strand at 5633.108 kb on + strand at 5633.110 kb on + strand at 5633.111 kb on - strand at 5633.114 kb on + strand at 5633.174 kb on + strand at 5633.207 kb on - strand at 5633.214 kb on - strand at 5633.218 kb on + strand at 5633.225 kb on + strand at 5633.226 kb on - strand at 5633.261 kb on - strand at 5633.332 kb on + strand at 5633.360 kb on - strand at 5633.377 kb on + strand at 5633.404 kb on + strand at 5633.444 kb on - strand at 5633.447 kb on + strand at 5633.447 kb on + strand at 5633.456 kb on + strand at 5633.471 kb on - strand at 5633.471 kb on - strand at 5633.567 kb on + strand at 5633.616 kb on + strand at 5633.618 kb on + strand at 5633.639 kb on + strand at 5633.678 kb on + strand at 5633.678 kb on + strand at 5633.731 kb on - strand at 5633.736 kb on + strand at 5633.750 kb on + strand at 5633.779 kb on + strand at 5633.783 kb on + strand at 5633.783 kb on + strand at 5633.784 kb on - strand at 5633.822 kb on - strand at 5633.846 kb on + strand, within BT4274 at 5633.859 kb on + strand, within BT4274 at 5633.894 kb on + strand, within BT4274 at 5633.923 kb on + strand, within BT4274 at 5633.940 kb on + strand, within BT4274 at 5633.987 kb on + strand, within BT4274 at 5633.988 kb on - strand, within BT4274 at 5633.988 kb on - strand, within BT4274 at 5634.007 kb on - strand, within BT4274 at 5634.049 kb on + strand, within BT4274 at 5634.056 kb on - strand, within BT4274 at 5634.094 kb on + strand, within BT4274 at 5634.145 kb on + strand, within BT4274 at 5634.177 kb on + strand, within BT4274 at 5634.177 kb on + strand, within BT4274 at 5634.181 kb on + strand, within BT4274 at 5634.226 kb on - strand, within BT4274 at 5634.316 kb on - strand, within BT4274 at 5634.323 kb on + strand, within BT4274 at 5634.356 kb on + strand, within BT4274 at 5634.406 kb on + strand, within BT4274 at 5634.415 kb on + strand, within BT4274 at 5634.415 kb on + strand, within BT4274 at 5634.437 kb on + strand, within BT4274 at 5634.437 kb on + strand, within BT4274 at 5634.458 kb on - strand, within BT4274 at 5634.507 kb on - strand at 5634.518 kb on + strand at 5634.519 kb on - strand at 5634.527 kb on + strand at 5634.527 kb on + strand at 5634.632 kb on - strand at 5634.632 kb on - strand at 5634.666 kb on - strand at 5634.672 kb on + strand at 5634.687 kb on + strand at 5634.710 kb on + strand at 5634.718 kb on + strand at 5634.719 kb on - strand at 5634.747 kb on - strand, within BT4275 at 5634.762 kb on + strand, within BT4275 at 5634.781 kb on + strand, within BT4275 at 5634.792 kb on + strand, within BT4275 at 5634.794 kb on + strand, within BT4275 at 5634.847 kb on - strand, within BT4275 at 5634.933 kb on + strand, within BT4275 at 5635.027 kb on - strand, within BT4275 at 5635.065 kb on - strand, within BT4275 at 5635.194 kb on + strand, within BT4275 at 5635.244 kb on - strand, within BT4275 at 5635.246 kb on + strand, within BT4275 at 5635.322 kb on + strand, within BT4275 at 5635.335 kb on + strand, within BT4275 at 5635.468 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 15 uM remove 5,632,840 + BT4273 0.80 -2.9 5,632,896 - BT4273 0.84 -1.3 5,632,926 + BT4273 0.85 -1.0 5,632,937 - BT4273 0.86 +2.0 5,632,937 - BT4273 0.86 +0.0 5,632,985 + BT4273 0.89 -1.0 5,632,988 + BT4273 0.89 -1.3 5,632,989 - BT4273 0.89 +0.0 5,633,066 - -1.6 5,633,084 - -1.8 5,633,108 + -2.1 5,633,110 + +0.0 5,633,111 - +2.0 5,633,114 + -1.1 5,633,174 + +0.0 5,633,207 - -1.0 5,633,214 - +2.6 5,633,218 + -1.3 5,633,225 + -1.0 5,633,226 - -0.6 5,633,261 - -1.6 5,633,332 + +1.2 5,633,360 - -1.6 5,633,377 + -0.3 5,633,404 + -2.0 5,633,444 - +0.0 5,633,447 + +0.0 5,633,447 + -1.1 5,633,456 + -1.0 5,633,471 - +0.6 5,633,471 - +2.0 5,633,567 + +1.6 5,633,616 + -1.0 5,633,618 + -2.5 5,633,639 + -1.8 5,633,678 + -1.0 5,633,678 + -1.6 5,633,731 - +0.0 5,633,736 + -2.9 5,633,750 + -2.0 5,633,779 + -1.8 5,633,783 + -1.6 5,633,783 + -3.3 5,633,784 - -2.0 5,633,822 - -1.6 5,633,846 + BT4274 0.11 +0.0 5,633,859 + BT4274 0.12 -2.0 5,633,894 + BT4274 0.17 +2.3 5,633,923 + BT4274 0.20 -2.6 5,633,940 + BT4274 0.22 +0.0 5,633,987 + BT4274 0.28 -0.6 5,633,988 - BT4274 0.29 -2.0 5,633,988 - BT4274 0.29 +0.0 5,634,007 - BT4274 0.31 -1.4 5,634,049 + BT4274 0.36 +0.0 5,634,056 - BT4274 0.37 -2.3 5,634,094 + BT4274 0.42 -2.0 5,634,145 + BT4274 0.48 -2.4 5,634,177 + BT4274 0.52 -0.3 5,634,177 + BT4274 0.52 -1.4 5,634,181 + BT4274 0.53 +0.0 5,634,226 - BT4274 0.59 +0.0 5,634,316 - BT4274 0.70 -2.6 5,634,323 + BT4274 0.71 -1.0 5,634,356 + BT4274 0.75 -1.3 5,634,406 + BT4274 0.81 -1.0 5,634,415 + BT4274 0.82 -1.6 5,634,415 + BT4274 0.82 +0.0 5,634,437 + BT4274 0.85 -2.4 5,634,437 + BT4274 0.85 -1.0 5,634,458 - BT4274 0.88 -1.0 5,634,507 - -0.3 5,634,518 + -2.3 5,634,519 - +0.0 5,634,527 + -2.3 5,634,527 + -1.1 5,634,632 - -0.6 5,634,632 - +1.0 5,634,666 - +0.0 5,634,672 + -2.6 5,634,687 + +0.0 5,634,710 + -1.0 5,634,718 + +0.7 5,634,719 - -1.2 5,634,747 - BT4275 0.11 -1.6 5,634,762 + BT4275 0.13 +0.2 5,634,781 + BT4275 0.15 +0.0 5,634,792 + BT4275 0.17 -1.3 5,634,794 + BT4275 0.17 -2.3 5,634,847 - BT4275 0.24 +3.2 5,634,933 + BT4275 0.35 -0.4 5,635,027 - BT4275 0.48 +0.4 5,635,065 - BT4275 0.53 +0.0 5,635,194 + BT4275 0.70 -1.0 5,635,244 - BT4275 0.76 -1.1 5,635,246 + BT4275 0.77 -0.6 5,635,322 + BT4275 0.87 -1.6 5,635,335 + BT4275 0.88 -1.6 5,635,468 - -1.0
Or see this region's nucleotide sequence