Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT4274

Experiment: BHIS with Trimethoprim 15 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT4273 and BT4274 are separated by 609 nucleotidesBT4274 and BT4275 are separated by 110 nucleotidesBT4275 and BT4276 are separated by 80 nucleotides BT4273: BT4273 - conserved hypothetical protein (NCBI ptt file), at 5,631,593 to 5,633,152 BT4273 BT4274: BT4274 - hypothetical protein (NCBI ptt file), at 5,633,762 to 5,634,553 BT4274 BT4275: BT4275 - hypothetical protein (NCBI ptt file), at 5,634,664 to 5,635,422 BT4275 BT4276: BT4276 - conserved hypothetical protein, putative transposase (NCBI ptt file), at 5,635,503 to 5,635,874 BT4276 Position (kb) 5633 5634 5635Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 5632.840 kb on + strand, within BT4273at 5632.896 kb on - strand, within BT4273at 5632.926 kb on + strand, within BT4273at 5632.937 kb on - strand, within BT4273at 5632.937 kb on - strand, within BT4273at 5632.985 kb on + strand, within BT4273at 5632.988 kb on + strand, within BT4273at 5632.989 kb on - strand, within BT4273at 5633.066 kb on - strandat 5633.084 kb on - strandat 5633.108 kb on + strandat 5633.110 kb on + strandat 5633.111 kb on - strandat 5633.114 kb on + strandat 5633.174 kb on + strandat 5633.207 kb on - strandat 5633.214 kb on - strandat 5633.218 kb on + strandat 5633.225 kb on + strandat 5633.226 kb on - strandat 5633.261 kb on - strandat 5633.332 kb on + strandat 5633.360 kb on - strandat 5633.377 kb on + strandat 5633.404 kb on + strandat 5633.444 kb on - strandat 5633.447 kb on + strandat 5633.447 kb on + strandat 5633.456 kb on + strandat 5633.471 kb on - strandat 5633.471 kb on - strandat 5633.567 kb on + strandat 5633.616 kb on + strandat 5633.618 kb on + strandat 5633.639 kb on + strandat 5633.678 kb on + strandat 5633.678 kb on + strandat 5633.731 kb on - strandat 5633.736 kb on + strandat 5633.750 kb on + strandat 5633.779 kb on + strandat 5633.783 kb on + strandat 5633.783 kb on + strandat 5633.784 kb on - strandat 5633.822 kb on - strandat 5633.846 kb on + strand, within BT4274at 5633.859 kb on + strand, within BT4274at 5633.894 kb on + strand, within BT4274at 5633.923 kb on + strand, within BT4274at 5633.940 kb on + strand, within BT4274at 5633.987 kb on + strand, within BT4274at 5633.988 kb on - strand, within BT4274at 5633.988 kb on - strand, within BT4274at 5634.007 kb on - strand, within BT4274at 5634.049 kb on + strand, within BT4274at 5634.056 kb on - strand, within BT4274at 5634.094 kb on + strand, within BT4274at 5634.145 kb on + strand, within BT4274at 5634.177 kb on + strand, within BT4274at 5634.177 kb on + strand, within BT4274at 5634.181 kb on + strand, within BT4274at 5634.226 kb on - strand, within BT4274at 5634.316 kb on - strand, within BT4274at 5634.323 kb on + strand, within BT4274at 5634.356 kb on + strand, within BT4274at 5634.406 kb on + strand, within BT4274at 5634.415 kb on + strand, within BT4274at 5634.415 kb on + strand, within BT4274at 5634.437 kb on + strand, within BT4274at 5634.437 kb on + strand, within BT4274at 5634.458 kb on - strand, within BT4274at 5634.507 kb on - strandat 5634.518 kb on + strandat 5634.519 kb on - strandat 5634.527 kb on + strandat 5634.527 kb on + strandat 5634.632 kb on - strandat 5634.632 kb on - strandat 5634.666 kb on - strandat 5634.672 kb on + strandat 5634.687 kb on + strandat 5634.710 kb on + strandat 5634.718 kb on + strandat 5634.719 kb on - strandat 5634.747 kb on - strand, within BT4275at 5634.762 kb on + strand, within BT4275at 5634.781 kb on + strand, within BT4275at 5634.792 kb on + strand, within BT4275at 5634.794 kb on + strand, within BT4275at 5634.847 kb on - strand, within BT4275at 5634.933 kb on + strand, within BT4275at 5635.027 kb on - strand, within BT4275at 5635.065 kb on - strand, within BT4275at 5635.194 kb on + strand, within BT4275at 5635.244 kb on - strand, within BT4275at 5635.246 kb on + strand, within BT4275at 5635.322 kb on + strand, within BT4275at 5635.335 kb on + strand, within BT4275at 5635.468 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 15 uM
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5,632,840 + BT4273 0.80 -2.9
5,632,896 - BT4273 0.84 -1.3
5,632,926 + BT4273 0.85 -1.0
5,632,937 - BT4273 0.86 +2.0
5,632,937 - BT4273 0.86 +0.0
5,632,985 + BT4273 0.89 -1.0
5,632,988 + BT4273 0.89 -1.3
5,632,989 - BT4273 0.89 +0.0
5,633,066 - -1.6
5,633,084 - -1.8
5,633,108 + -2.1
5,633,110 + +0.0
5,633,111 - +2.0
5,633,114 + -1.1
5,633,174 + +0.0
5,633,207 - -1.0
5,633,214 - +2.6
5,633,218 + -1.3
5,633,225 + -1.0
5,633,226 - -0.6
5,633,261 - -1.6
5,633,332 + +1.2
5,633,360 - -1.6
5,633,377 + -0.3
5,633,404 + -2.0
5,633,444 - +0.0
5,633,447 + +0.0
5,633,447 + -1.1
5,633,456 + -1.0
5,633,471 - +0.6
5,633,471 - +2.0
5,633,567 + +1.6
5,633,616 + -1.0
5,633,618 + -2.5
5,633,639 + -1.8
5,633,678 + -1.0
5,633,678 + -1.6
5,633,731 - +0.0
5,633,736 + -2.9
5,633,750 + -2.0
5,633,779 + -1.8
5,633,783 + -1.6
5,633,783 + -3.3
5,633,784 - -2.0
5,633,822 - -1.6
5,633,846 + BT4274 0.11 +0.0
5,633,859 + BT4274 0.12 -2.0
5,633,894 + BT4274 0.17 +2.3
5,633,923 + BT4274 0.20 -2.6
5,633,940 + BT4274 0.22 +0.0
5,633,987 + BT4274 0.28 -0.6
5,633,988 - BT4274 0.29 -2.0
5,633,988 - BT4274 0.29 +0.0
5,634,007 - BT4274 0.31 -1.4
5,634,049 + BT4274 0.36 +0.0
5,634,056 - BT4274 0.37 -2.3
5,634,094 + BT4274 0.42 -2.0
5,634,145 + BT4274 0.48 -2.4
5,634,177 + BT4274 0.52 -0.3
5,634,177 + BT4274 0.52 -1.4
5,634,181 + BT4274 0.53 +0.0
5,634,226 - BT4274 0.59 +0.0
5,634,316 - BT4274 0.70 -2.6
5,634,323 + BT4274 0.71 -1.0
5,634,356 + BT4274 0.75 -1.3
5,634,406 + BT4274 0.81 -1.0
5,634,415 + BT4274 0.82 -1.6
5,634,415 + BT4274 0.82 +0.0
5,634,437 + BT4274 0.85 -2.4
5,634,437 + BT4274 0.85 -1.0
5,634,458 - BT4274 0.88 -1.0
5,634,507 - -0.3
5,634,518 + -2.3
5,634,519 - +0.0
5,634,527 + -2.3
5,634,527 + -1.1
5,634,632 - -0.6
5,634,632 - +1.0
5,634,666 - +0.0
5,634,672 + -2.6
5,634,687 + +0.0
5,634,710 + -1.0
5,634,718 + +0.7
5,634,719 - -1.2
5,634,747 - BT4275 0.11 -1.6
5,634,762 + BT4275 0.13 +0.2
5,634,781 + BT4275 0.15 +0.0
5,634,792 + BT4275 0.17 -1.3
5,634,794 + BT4275 0.17 -2.3
5,634,847 - BT4275 0.24 +3.2
5,634,933 + BT4275 0.35 -0.4
5,635,027 - BT4275 0.48 +0.4
5,635,065 - BT4275 0.53 +0.0
5,635,194 + BT4275 0.70 -1.0
5,635,244 - BT4275 0.76 -1.1
5,635,246 + BT4275 0.77 -0.6
5,635,322 + BT4275 0.87 -1.6
5,635,335 + BT4275 0.88 -1.6
5,635,468 - -1.0

Or see this region's nucleotide sequence