Experiment: BHIS with Trimethoprim 15 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0508 and BT0509 overlap by 4 nucleotides BT0509 and BT0510 are separated by 321 nucleotides
BT0508: BT0508 - ABC transporter ATP-binding protein (NCBI ptt file), at 629,904 to 631,733
BT0508
BT0509: BT0509 - ABC transporter ATP-binding/transmembrane protein (NCBI ptt file), at 631,730 to 633,460
BT0509
BT0510: BT0510 - putative heme biosynthesis protein (NCBI ptt file), at 633,782 to 634,882
BT0510
Position (kb) 631
632
633
634 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 630.737 kb on - strand, within BT0508 at 631.257 kb on - strand, within BT0508 at 631.548 kb on - strand, within BT0508 at 631.588 kb on + strand at 631.615 kb on + strand at 631.657 kb on + strand at 631.658 kb on - strand at 631.662 kb on - strand at 631.662 kb on - strand at 631.681 kb on - strand at 631.683 kb on - strand at 631.848 kb on + strand at 631.922 kb on - strand, within BT0509 at 631.936 kb on + strand, within BT0509 at 631.994 kb on + strand, within BT0509 at 632.042 kb on - strand, within BT0509 at 632.110 kb on + strand, within BT0509 at 632.131 kb on - strand, within BT0509 at 632.232 kb on + strand, within BT0509 at 632.416 kb on + strand, within BT0509 at 632.417 kb on - strand, within BT0509 at 632.642 kb on + strand, within BT0509 at 632.665 kb on + strand, within BT0509 at 632.672 kb on - strand, within BT0509 at 632.672 kb on - strand, within BT0509 at 632.745 kb on + strand, within BT0509 at 632.746 kb on - strand, within BT0509 at 632.876 kb on + strand, within BT0509 at 632.877 kb on - strand, within BT0509 at 632.978 kb on + strand, within BT0509 at 632.979 kb on - strand, within BT0509 at 633.104 kb on + strand, within BT0509 at 633.105 kb on - strand, within BT0509 at 633.110 kb on - strand, within BT0509 at 633.246 kb on + strand, within BT0509 at 633.428 kb on - strand at 633.545 kb on + strand at 633.545 kb on + strand at 633.552 kb on + strand at 633.567 kb on + strand at 633.567 kb on + strand at 633.674 kb on - strand at 633.698 kb on + strand at 633.762 kb on - strand at 633.831 kb on - strand at 633.850 kb on + strand at 633.962 kb on + strand, within BT0510 at 633.962 kb on + strand, within BT0510 at 633.988 kb on + strand, within BT0510 at 634.091 kb on - strand, within BT0510 at 634.097 kb on + strand, within BT0510 at 634.111 kb on - strand, within BT0510 at 634.262 kb on + strand, within BT0510 at 634.263 kb on - strand, within BT0510 at 634.291 kb on + strand, within BT0510 at 634.373 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 15 uM remove 630,737 - BT0508 0.46 -1.0 631,257 - BT0508 0.74 +0.0 631,548 - BT0508 0.90 -2.3 631,588 + -2.6 631,615 + -1.6 631,657 + -3.0 631,658 - -1.2 631,662 - +0.0 631,662 - +0.0 631,681 - -1.0 631,683 - -1.6 631,848 + +0.0 631,922 - BT0509 0.11 -1.0 631,936 + BT0509 0.12 -1.6 631,994 + BT0509 0.15 -2.0 632,042 - BT0509 0.18 +0.0 632,110 + BT0509 0.22 +2.0 632,131 - BT0509 0.23 +0.6 632,232 + BT0509 0.29 -2.8 632,416 + BT0509 0.40 -1.0 632,417 - BT0509 0.40 -3.8 632,642 + BT0509 0.53 -2.0 632,665 + BT0509 0.54 -0.9 632,672 - BT0509 0.54 -1.4 632,672 - BT0509 0.54 -1.0 632,745 + BT0509 0.59 +0.0 632,746 - BT0509 0.59 +0.8 632,876 + BT0509 0.66 +0.0 632,877 - BT0509 0.66 -1.0 632,978 + BT0509 0.72 -2.0 632,979 - BT0509 0.72 -1.4 633,104 + BT0509 0.79 -1.0 633,105 - BT0509 0.79 -1.3 633,110 - BT0509 0.80 -3.9 633,246 + BT0509 0.88 -1.8 633,428 - +0.0 633,545 + -2.6 633,545 + -1.0 633,552 + -1.9 633,567 + +0.6 633,567 + +1.4 633,674 - +0.6 633,698 + -2.0 633,762 - -2.3 633,831 - +0.0 633,850 + +0.0 633,962 + BT0510 0.16 -2.1 633,962 + BT0510 0.16 -1.0 633,988 + BT0510 0.19 -1.6 634,091 - BT0510 0.28 -0.4 634,097 + BT0510 0.29 -1.6 634,111 - BT0510 0.30 -1.0 634,262 + BT0510 0.44 +0.0 634,263 - BT0510 0.44 +2.4 634,291 + BT0510 0.46 -2.3 634,373 - +2.0
Or see this region's nucleotide sequence