Experiment: Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16900 and BWI76_RS16905 overlap by 4 nucleotides BWI76_RS16905 and BWI76_RS16910 overlap by 8 nucleotides BWI76_RS16910 and BWI76_RS16915 are separated by 9 nucleotides BWI76_RS16915 and BWI76_RS16920 are separated by 17 nucleotides
BWI76_RS16900: BWI76_RS16900 - 4-hydroxy-2-oxovalerate aldolase, at 3,504,598 to 3,505,614
_RS16900
BWI76_RS16905: BWI76_RS16905 - acetaldehyde dehydrogenase (acetylating), at 3,505,611 to 3,506,561
_RS16905
BWI76_RS16910: BWI76_RS16910 - 2-keto-4-pentenoate hydratase, at 3,506,554 to 3,507,360
_RS16910
BWI76_RS16915: BWI76_RS16915 - alpha/beta hydrolase fold protein, at 3,507,370 to 3,508,236
_RS16915
BWI76_RS16920: BWI76_RS16920 - 3-(2,3-dihydroxyphenyl)propionate dioxygenase, at 3,508,254 to 3,509,198
_RS16920
Position (kb) 3506
3507
3508 Strain fitness (log2 ratio) -2
-1
0
1 at 3506.110 kb on - strand, within BWI76_RS16905 at 3506.206 kb on + strand, within BWI76_RS16905 at 3506.232 kb on + strand, within BWI76_RS16905 at 3506.287 kb on - strand, within BWI76_RS16905 at 3506.575 kb on - strand at 3506.594 kb on - strand at 3506.986 kb on - strand, within BWI76_RS16910 at 3507.250 kb on - strand, within BWI76_RS16910 at 3507.409 kb on + strand at 3507.410 kb on - strand at 3507.823 kb on - strand, within BWI76_RS16915 at 3507.854 kb on + strand, within BWI76_RS16915 at 3508.140 kb on + strand, within BWI76_RS16915
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 3,506,110 - BWI76_RS16905 0.52 +0.0 3,506,206 + BWI76_RS16905 0.63 +0.4 3,506,232 + BWI76_RS16905 0.65 -0.0 3,506,287 - BWI76_RS16905 0.71 -0.0 3,506,575 - +0.1 3,506,594 - +0.5 3,506,986 - BWI76_RS16910 0.54 -2.0 3,507,250 - BWI76_RS16910 0.86 +0.4 3,507,409 + +1.2 3,507,410 - -0.1 3,507,823 - BWI76_RS16915 0.52 +0.4 3,507,854 + BWI76_RS16915 0.56 +0.1 3,508,140 + BWI76_RS16915 0.89 +1.1
Or see this region's nucleotide sequence