Experiment: Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08115 and BWI76_RS08120 are separated by 22 nucleotides BWI76_RS08120 and BWI76_RS08125 are separated by 98 nucleotides BWI76_RS08125 and BWI76_RS08130 overlap by 4 nucleotides
BWI76_RS08115: BWI76_RS08115 - apolipoprotein N-acyltransferase, at 1,719,938 to 1,721,476
_RS08115
BWI76_RS08120: BWI76_RS08120 - cobalt transporter, at 1,721,499 to 1,722,377
_RS08120
BWI76_RS08125: BWI76_RS08125 - rRNA maturation RNase YbeY, at 1,722,476 to 1,722,943
_RS08125
BWI76_RS08130: BWI76_RS08130 - nucleoside triphosphate hydrolase, at 1,722,940 to 1,723,986
_RS08130
Position (kb) 1721
1722
1723 Strain fitness (log2 ratio) -1
0
1
2 at 1721.755 kb on - strand, within BWI76_RS08120 at 1721.839 kb on - strand, within BWI76_RS08120 at 1722.199 kb on - strand, within BWI76_RS08120 at 1722.482 kb on - strand at 1723.209 kb on - strand, within BWI76_RS08130
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 1,721,755 - BWI76_RS08120 0.29 +1.8 1,721,839 - BWI76_RS08120 0.39 +0.6 1,722,199 - BWI76_RS08120 0.80 +0.9 1,722,482 - +1.9 1,723,209 - BWI76_RS08130 0.26 -0.6
Or see this region's nucleotide sequence