Experiment: BHIS with Trimethoprim 15 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2489 and BT2490 are separated by 419 nucleotides BT2490 and BT2491 are separated by 59 nucleotides BT2491 and BT2492 are separated by 7 nucleotides
BT2489: BT2489 - 50S ribosomal protein L19 (NCBI ptt file), at 3,113,000 to 3,113,353
BT2489
BT2490: BT2490 - hypothetical protein (NCBI ptt file), at 3,113,773 to 3,114,066
BT2490
BT2491: BT2491 - hypothetical protein (NCBI ptt file), at 3,114,126 to 3,114,707
BT2491
BT2492: BT2492 - RNA polymerase ECF-type sigma factor (NCBI ptt file), at 3,114,715 to 3,115,191
BT2492
Position (kb)
3113
3114
3115 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3112.796 kb on + strand at 3112.803 kb on - strand at 3113.466 kb on - strand at 3113.479 kb on + strand at 3113.480 kb on - strand at 3113.486 kb on - strand at 3113.487 kb on + strand at 3113.487 kb on + strand at 3113.488 kb on - strand at 3113.488 kb on - strand at 3113.493 kb on + strand at 3113.498 kb on + strand at 3113.518 kb on + strand at 3113.518 kb on + strand at 3113.518 kb on + strand at 3113.519 kb on - strand at 3113.553 kb on + strand at 3113.555 kb on + strand at 3113.612 kb on + strand at 3113.618 kb on - strand at 3113.656 kb on - strand at 3113.687 kb on + strand at 3113.687 kb on + strand at 3113.688 kb on - strand at 3113.719 kb on + strand at 3113.720 kb on - strand at 3113.826 kb on - strand, within BT2490 at 3113.827 kb on + strand, within BT2490 at 3113.828 kb on - strand, within BT2490 at 3113.840 kb on + strand, within BT2490 at 3113.922 kb on - strand, within BT2490 at 3114.035 kb on + strand, within BT2490 at 3114.073 kb on - strand at 3114.081 kb on - strand at 3114.094 kb on - strand at 3114.138 kb on - strand at 3114.723 kb on + strand at 3114.748 kb on - strand at 3114.790 kb on + strand, within BT2492 at 3114.795 kb on + strand, within BT2492 at 3114.795 kb on + strand, within BT2492 at 3114.835 kb on + strand, within BT2492 at 3114.835 kb on + strand, within BT2492 at 3114.835 kb on + strand, within BT2492 at 3114.836 kb on - strand, within BT2492 at 3114.850 kb on - strand at 3114.882 kb on - strand, within BT2492 at 3114.904 kb on - strand, within BT2492 at 3114.991 kb on - strand, within BT2492 at 3114.991 kb on - strand, within BT2492 at 3115.001 kb on - strand, within BT2492 at 3115.021 kb on + strand, within BT2492 at 3115.023 kb on + strand, within BT2492 at 3115.023 kb on + strand, within BT2492 at 3115.026 kb on + strand, within BT2492 at 3115.044 kb on + strand, within BT2492
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 15 uM remove 3,112,796 + -3.0 3,112,803 - -1.0 3,113,466 - -2.0 3,113,479 + -1.2 3,113,480 - -0.8 3,113,486 - +1.4 3,113,487 + +0.2 3,113,487 + +1.0 3,113,488 - -0.9 3,113,488 - -1.3 3,113,493 + +2.3 3,113,498 + -0.8 3,113,518 + -1.8 3,113,518 + -1.1 3,113,518 + -1.2 3,113,519 - -0.6 3,113,553 + -1.8 3,113,555 + -0.0 3,113,612 + -0.6 3,113,618 - +1.0 3,113,656 - -1.6 3,113,687 + +0.2 3,113,687 + -1.6 3,113,688 - -0.0 3,113,719 + -1.4 3,113,720 - +2.0 3,113,826 - BT2490 0.18 +1.0 3,113,827 + BT2490 0.18 -0.6 3,113,828 - BT2490 0.19 +2.6 3,113,840 + BT2490 0.23 -1.8 3,113,922 - BT2490 0.51 +1.0 3,114,035 + BT2490 0.89 +1.3 3,114,073 - -0.0 3,114,081 - -2.0 3,114,094 - -0.7 3,114,138 - +0.7 3,114,723 + +2.0 3,114,748 - +1.0 3,114,790 + BT2492 0.16 -1.8 3,114,795 + BT2492 0.17 +0.3 3,114,795 + BT2492 0.17 -2.5 3,114,835 + BT2492 0.25 +0.2 3,114,835 + BT2492 0.25 +0.3 3,114,835 + BT2492 0.25 -0.0 3,114,836 - BT2492 0.25 -1.0 3,114,850 - -1.0 3,114,882 - BT2492 0.35 +0.3 3,114,904 - BT2492 0.40 -1.0 3,114,991 - BT2492 0.58 -1.2 3,114,991 - BT2492 0.58 -0.0 3,115,001 - BT2492 0.60 -1.3 3,115,021 + BT2492 0.64 +0.6 3,115,023 + BT2492 0.65 -1.5 3,115,023 + BT2492 0.65 -1.2 3,115,026 + BT2492 0.65 -1.1 3,115,044 + BT2492 0.69 -0.0
Or see this region's nucleotide sequence