Experiment: LB anaerobic; ground control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1224 and SO1225 are separated by 12 nucleotides SO1225 and radA are separated by 158 nucleotides
SO1224: SO1224 - conserved hypothetical protein (NCBI ptt file), at 1,270,673 to 1,271,659
SO1224
SO1225: SO1225 - conserved hypothetical protein (NCBI ptt file), at 1,271,672 to 1,274,050
SO1225
SO1226: radA - DNA repair protein RadA (NCBI ptt file), at 1,274,209 to 1,275,573
radA
Position (kb)
1271
1272
1273
1274
1275 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 1270.718 kb on + strand at 1270.966 kb on - strand, within SO1224 at 1271.043 kb on + strand, within SO1224 at 1271.133 kb on + strand, within SO1224 at 1271.277 kb on - strand, within SO1224 at 1271.355 kb on - strand, within SO1224 at 1271.357 kb on + strand, within SO1224 at 1271.453 kb on + strand, within SO1224 at 1271.453 kb on + strand, within SO1224 at 1271.453 kb on + strand, within SO1224 at 1271.453 kb on + strand, within SO1224 at 1271.461 kb on - strand, within SO1224 at 1271.461 kb on - strand, within SO1224 at 1271.461 kb on - strand, within SO1224 at 1271.461 kb on - strand, within SO1224 at 1271.461 kb on - strand, within SO1224 at 1271.653 kb on + strand at 1271.653 kb on - strand at 1271.661 kb on - strand at 1271.756 kb on + strand at 1271.828 kb on + strand at 1271.935 kb on + strand, within SO1225 at 1272.151 kb on + strand, within SO1225 at 1272.151 kb on + strand, within SO1225 at 1272.163 kb on + strand, within SO1225 at 1272.163 kb on + strand, within SO1225 at 1272.171 kb on - strand, within SO1225 at 1272.171 kb on - strand, within SO1225 at 1272.171 kb on - strand, within SO1225 at 1272.362 kb on + strand, within SO1225 at 1272.370 kb on - strand, within SO1225 at 1272.404 kb on + strand, within SO1225 at 1272.404 kb on + strand, within SO1225 at 1272.412 kb on - strand, within SO1225 at 1272.413 kb on - strand, within SO1225 at 1272.463 kb on - strand, within SO1225 at 1272.486 kb on + strand, within SO1225 at 1272.503 kb on - strand, within SO1225 at 1272.519 kb on + strand, within SO1225 at 1272.541 kb on - strand, within SO1225 at 1272.568 kb on + strand, within SO1225 at 1272.575 kb on - strand, within SO1225 at 1272.575 kb on - strand, within SO1225 at 1272.576 kb on - strand, within SO1225 at 1272.857 kb on - strand, within SO1225 at 1272.869 kb on - strand, within SO1225 at 1272.869 kb on - strand, within SO1225 at 1272.957 kb on - strand, within SO1225 at 1272.958 kb on - strand, within SO1225 at 1272.998 kb on + strand, within SO1225 at 1272.998 kb on + strand, within SO1225 at 1272.998 kb on + strand, within SO1225 at 1272.998 kb on + strand, within SO1225 at 1272.998 kb on + strand, within SO1225 at 1272.998 kb on + strand, within SO1225 at 1272.998 kb on + strand, within SO1225 at 1272.998 kb on + strand, within SO1225 at 1272.998 kb on + strand, within SO1225 at 1273.006 kb on - strand, within SO1225 at 1273.006 kb on - strand, within SO1225 at 1273.006 kb on - strand, within SO1225 at 1273.006 kb on - strand, within SO1225 at 1273.006 kb on - strand, within SO1225 at 1273.006 kb on - strand, within SO1225 at 1273.039 kb on - strand, within SO1225 at 1273.044 kb on - strand, within SO1225 at 1273.055 kb on + strand, within SO1225 at 1273.055 kb on + strand, within SO1225 at 1273.055 kb on + strand, within SO1225 at 1273.063 kb on - strand, within SO1225 at 1273.241 kb on + strand, within SO1225 at 1273.241 kb on + strand, within SO1225 at 1273.254 kb on - strand, within SO1225 at 1273.302 kb on - strand, within SO1225 at 1273.506 kb on - strand, within SO1225 at 1273.537 kb on + strand, within SO1225 at 1273.663 kb on - strand, within SO1225 at 1273.750 kb on + strand, within SO1225 at 1273.839 kb on + strand at 1273.859 kb on - strand at 1273.861 kb on + strand at 1273.869 kb on - strand at 1273.869 kb on - strand at 1273.869 kb on - strand at 1273.869 kb on - strand at 1273.879 kb on + strand at 1273.970 kb on - strand at 1274.015 kb on - strand at 1274.024 kb on - strand at 1274.143 kb on + strand at 1274.143 kb on + strand at 1274.165 kb on + strand at 1274.217 kb on + strand at 1274.225 kb on - strand at 1274.225 kb on - strand at 1274.272 kb on + strand at 1274.272 kb on + strand at 1274.280 kb on - strand at 1274.364 kb on + strand, within radA at 1274.386 kb on + strand, within radA at 1274.443 kb on + strand, within radA at 1274.551 kb on - strand, within radA at 1274.642 kb on + strand, within radA at 1274.680 kb on - strand, within radA at 1274.805 kb on + strand, within radA at 1274.842 kb on - strand, within radA at 1275.026 kb on + strand, within radA at 1275.026 kb on + strand, within radA at 1275.026 kb on + strand, within radA at 1275.026 kb on + strand, within radA at 1275.034 kb on - strand, within radA at 1275.034 kb on - strand, within radA at 1275.034 kb on - strand, within radA at 1275.034 kb on - strand, within radA at 1275.047 kb on - strand, within radA
Per-strain Table
Position Strand Gene LocusTag Fraction LB anaerobic; ground control remove 1,270,718 + +4.9 1,270,966 - SO1224 0.30 -0.2 1,271,043 + SO1224 0.37 +1.4 1,271,133 + SO1224 0.47 +0.1 1,271,277 - SO1224 0.61 +1.2 1,271,355 - SO1224 0.69 +0.1 1,271,357 + SO1224 0.69 +1.1 1,271,453 + SO1224 0.79 +0.7 1,271,453 + SO1224 0.79 +0.6 1,271,453 + SO1224 0.79 -0.3 1,271,453 + SO1224 0.79 -0.2 1,271,461 - SO1224 0.80 -0.4 1,271,461 - SO1224 0.80 -3.5 1,271,461 - SO1224 0.80 -0.3 1,271,461 - SO1224 0.80 -1.5 1,271,461 - SO1224 0.80 -1.1 1,271,653 + -0.2 1,271,653 - +0.3 1,271,661 - -2.0 1,271,756 + +1.0 1,271,828 + +3.2 1,271,935 + SO1225 0.11 +1.0 1,272,151 + SO1225 0.20 +0.2 1,272,151 + SO1225 0.20 -0.2 1,272,163 + SO1225 0.21 +2.1 1,272,163 + SO1225 0.21 -3.5 1,272,171 - SO1225 0.21 +0.9 1,272,171 - SO1225 0.21 +0.7 1,272,171 - SO1225 0.21 +1.3 1,272,362 + SO1225 0.29 +0.3 1,272,370 - SO1225 0.29 +0.4 1,272,404 + SO1225 0.31 -0.1 1,272,404 + SO1225 0.31 +0.4 1,272,412 - SO1225 0.31 -0.3 1,272,413 - SO1225 0.31 +2.9 1,272,463 - SO1225 0.33 -0.1 1,272,486 + SO1225 0.34 +1.3 1,272,503 - SO1225 0.35 +0.3 1,272,519 + SO1225 0.36 -0.6 1,272,541 - SO1225 0.37 -0.5 1,272,568 + SO1225 0.38 -0.9 1,272,575 - SO1225 0.38 -2.3 1,272,575 - SO1225 0.38 +0.2 1,272,576 - SO1225 0.38 +1.3 1,272,857 - SO1225 0.50 -0.4 1,272,869 - SO1225 0.50 -0.9 1,272,869 - SO1225 0.50 -0.2 1,272,957 - SO1225 0.54 -0.7 1,272,958 - SO1225 0.54 -0.9 1,272,998 + SO1225 0.56 -0.1 1,272,998 + SO1225 0.56 +0.4 1,272,998 + SO1225 0.56 -0.6 1,272,998 + SO1225 0.56 +2.0 1,272,998 + SO1225 0.56 +0.7 1,272,998 + SO1225 0.56 +0.5 1,272,998 + SO1225 0.56 -1.1 1,272,998 + SO1225 0.56 -0.3 1,272,998 + SO1225 0.56 +0.9 1,273,006 - SO1225 0.56 +0.9 1,273,006 - SO1225 0.56 -0.3 1,273,006 - SO1225 0.56 +1.1 1,273,006 - SO1225 0.56 -3.7 1,273,006 - SO1225 0.56 +1.7 1,273,006 - SO1225 0.56 +2.9 1,273,039 - SO1225 0.57 +1.0 1,273,044 - SO1225 0.58 -0.5 1,273,055 + SO1225 0.58 +1.9 1,273,055 + SO1225 0.58 +1.1 1,273,055 + SO1225 0.58 +0.6 1,273,063 - SO1225 0.58 -3.0 1,273,241 + SO1225 0.66 +0.8 1,273,241 + SO1225 0.66 +0.9 1,273,254 - SO1225 0.66 -0.3 1,273,302 - SO1225 0.69 +1.3 1,273,506 - SO1225 0.77 -0.4 1,273,537 + SO1225 0.78 +0.1 1,273,663 - SO1225 0.84 -3.0 1,273,750 + SO1225 0.87 -0.6 1,273,839 + +0.4 1,273,859 - +1.6 1,273,861 + +0.7 1,273,869 - +0.8 1,273,869 - +0.6 1,273,869 - -1.2 1,273,869 - -1.9 1,273,879 + +1.3 1,273,970 - +0.1 1,274,015 - +0.5 1,274,024 - +0.6 1,274,143 + +0.5 1,274,143 + +0.4 1,274,165 + +1.3 1,274,217 + +1.0 1,274,225 - -0.4 1,274,225 - +0.3 1,274,272 + -3.5 1,274,272 + +0.3 1,274,280 - +1.9 1,274,364 + radA SO1226 0.11 +1.2 1,274,386 + radA SO1226 0.13 +0.9 1,274,443 + radA SO1226 0.17 -1.3 1,274,551 - radA SO1226 0.25 -0.4 1,274,642 + radA SO1226 0.32 +2.3 1,274,680 - radA SO1226 0.35 -1.3 1,274,805 + radA SO1226 0.44 -0.3 1,274,842 - radA SO1226 0.46 +0.9 1,275,026 + radA SO1226 0.60 -3.2 1,275,026 + radA SO1226 0.60 +0.8 1,275,026 + radA SO1226 0.60 -1.7 1,275,026 + radA SO1226 0.60 -1.4 1,275,034 - radA SO1226 0.60 -0.1 1,275,034 - radA SO1226 0.60 -1.7 1,275,034 - radA SO1226 0.60 -2.4 1,275,034 - radA SO1226 0.60 +0.3 1,275,047 - radA SO1226 0.61 +1.1
Or see this region's nucleotide sequence