Experiment: Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26400 and BWI76_RS26405 are separated by 192 nucleotides BWI76_RS26405 and BWI76_RS26410 overlap by 11 nucleotides BWI76_RS26410 and BWI76_RS26415 are separated by 14 nucleotides BWI76_RS26415 and BWI76_RS26420 are separated by 142 nucleotides
BWI76_RS26400: BWI76_RS26400 - signal recognition particle-docking protein FtsY, at 5,465,039 to 5,466,553
_RS26400
BWI76_RS26405: BWI76_RS26405 - 16S rRNA (guanine(966)-N(2))-methyltransferase, at 5,466,746 to 5,467,342
_RS26405
BWI76_RS26410: BWI76_RS26410 - membrane protein, at 5,467,332 to 5,467,610
_RS26410
BWI76_RS26415: BWI76_RS26415 - hypothetical protein, at 5,467,625 to 5,467,984
_RS26415
BWI76_RS26420: BWI76_RS26420 - lysoplasmalogenase, at 5,468,127 to 5,468,753
_RS26420
Position (kb) 5466
5467
5468 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5466.588 kb on - strand at 5466.676 kb on + strand at 5466.953 kb on - strand, within BWI76_RS26405 at 5467.051 kb on + strand, within BWI76_RS26405 at 5467.305 kb on + strand at 5467.437 kb on + strand, within BWI76_RS26410 at 5467.473 kb on - strand, within BWI76_RS26410 at 5467.659 kb on + strand at 5467.753 kb on + strand, within BWI76_RS26415 at 5468.015 kb on + strand at 5468.165 kb on + strand at 5468.165 kb on + strand at 5468.173 kb on - strand at 5468.173 kb on - strand at 5468.227 kb on + strand, within BWI76_RS26420 at 5468.325 kb on + strand, within BWI76_RS26420 at 5468.342 kb on - strand, within BWI76_RS26420
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 5,466,588 - +1.2 5,466,676 + +0.0 5,466,953 - BWI76_RS26405 0.35 +1.1 5,467,051 + BWI76_RS26405 0.51 +1.6 5,467,305 + -1.0 5,467,437 + BWI76_RS26410 0.38 +1.8 5,467,473 - BWI76_RS26410 0.51 -0.9 5,467,659 + -0.5 5,467,753 + BWI76_RS26415 0.36 +1.2 5,468,015 + +0.4 5,468,165 + +0.9 5,468,165 + -0.1 5,468,173 - -1.2 5,468,173 - -1.3 5,468,227 + BWI76_RS26420 0.16 +0.3 5,468,325 + BWI76_RS26420 0.32 +0.1 5,468,342 - BWI76_RS26420 0.34 -3.3
Or see this region's nucleotide sequence