Experiment: Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15290 and BWI76_RS15295 are separated by 17 nucleotides BWI76_RS15295 and BWI76_RS15300 are separated by 30 nucleotides BWI76_RS15300 and BWI76_RS15305 are separated by 31 nucleotides
BWI76_RS15290: BWI76_RS15290 - transcriptional regulator, at 3,204,237 to 3,204,671
_RS15290
BWI76_RS15295: BWI76_RS15295 - MDR efflux pump AcrAB transcriptional activator MarA, at 3,204,689 to 3,205,069
_RS15295
BWI76_RS15300: BWI76_RS15300 - multiple antibiotic resistance regulatory periplasmic protein MarB, at 3,205,100 to 3,205,318
_RS15300
BWI76_RS15305: BWI76_RS15305 - O-acetylserine/cysteine exporter, at 3,205,350 to 3,206,249
_RS15305
Position (kb) 3205
3206 Strain fitness (log2 ratio) -1
0
1
2 at 3204.104 kb on + strand at 3204.104 kb on + strand at 3204.122 kb on - strand at 3204.393 kb on + strand, within BWI76_RS15290 at 3204.632 kb on + strand at 3204.875 kb on - strand, within BWI76_RS15295 at 3204.875 kb on - strand, within BWI76_RS15295 at 3204.917 kb on + strand, within BWI76_RS15295 at 3204.918 kb on + strand, within BWI76_RS15295 at 3204.926 kb on - strand, within BWI76_RS15295 at 3205.029 kb on - strand, within BWI76_RS15295 at 3205.150 kb on - strand, within BWI76_RS15300 at 3205.156 kb on - strand, within BWI76_RS15300 at 3205.163 kb on + strand, within BWI76_RS15300 at 3205.171 kb on - strand, within BWI76_RS15300 at 3205.256 kb on + strand, within BWI76_RS15300 at 3205.296 kb on + strand, within BWI76_RS15300 at 3205.560 kb on - strand, within BWI76_RS15305 at 3205.788 kb on + strand, within BWI76_RS15305 at 3206.111 kb on - strand, within BWI76_RS15305 at 3206.314 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 3,204,104 + +0.4 3,204,104 + +0.3 3,204,122 - +0.7 3,204,393 + BWI76_RS15290 0.36 +0.5 3,204,632 + +0.6 3,204,875 - BWI76_RS15295 0.49 +0.4 3,204,875 - BWI76_RS15295 0.49 +0.4 3,204,917 + BWI76_RS15295 0.60 +0.6 3,204,918 + BWI76_RS15295 0.60 +0.4 3,204,926 - BWI76_RS15295 0.62 +0.5 3,205,029 - BWI76_RS15295 0.89 -0.3 3,205,150 - BWI76_RS15300 0.23 +0.3 3,205,156 - BWI76_RS15300 0.26 +2.0 3,205,163 + BWI76_RS15300 0.29 +0.7 3,205,171 - BWI76_RS15300 0.32 -0.9 3,205,256 + BWI76_RS15300 0.71 +1.2 3,205,296 + BWI76_RS15300 0.89 +0.6 3,205,560 - BWI76_RS15305 0.23 +0.2 3,205,788 + BWI76_RS15305 0.49 +0.2 3,206,111 - BWI76_RS15305 0.85 -0.5 3,206,314 + +0.8
Or see this region's nucleotide sequence