Experiment: Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11230 and BWI76_RS11235 are separated by 28 nucleotides BWI76_RS11235 and BWI76_RS11240 are separated by 346 nucleotides BWI76_RS11240 and BWI76_RS11245 overlap by 4 nucleotides BWI76_RS11245 and BWI76_RS11250 overlap by 4 nucleotides
BWI76_RS11230: BWI76_RS11230 - NAD-dependent deacylase, at 2,369,681 to 2,370,526
_RS11230
BWI76_RS11235: BWI76_RS11235 - hypothetical protein, at 2,370,555 to 2,370,860
_RS11235
BWI76_RS11240: BWI76_RS11240 - spermidine/putrescine ABC transporter substrate-binding protein PotD, at 2,371,207 to 2,372,253
_RS11240
BWI76_RS11245: BWI76_RS11245 - spermidine/putrescine ABC transporter permease PotC, at 2,372,250 to 2,373,035
_RS11245
BWI76_RS11250: BWI76_RS11250 - spermidine/putrescine ABC transporter permease PotB, at 2,373,032 to 2,373,889
_RS11250
Position (kb)
2371
2372
2373 Strain fitness (log2 ratio)
-1
0
1 at 2370.211 kb on - strand, within BWI76_RS11230 at 2370.335 kb on - strand, within BWI76_RS11230 at 2370.442 kb on + strand at 2370.552 kb on + strand at 2370.560 kb on - strand at 2370.623 kb on + strand, within BWI76_RS11235 at 2370.904 kb on + strand at 2371.030 kb on + strand at 2371.166 kb on - strand at 2371.571 kb on + strand, within BWI76_RS11240 at 2371.715 kb on + strand, within BWI76_RS11240 at 2371.725 kb on + strand, within BWI76_RS11240 at 2371.762 kb on - strand, within BWI76_RS11240 at 2371.867 kb on - strand, within BWI76_RS11240 at 2372.461 kb on - strand, within BWI76_RS11245 at 2372.599 kb on - strand, within BWI76_RS11245 at 2372.922 kb on + strand, within BWI76_RS11245
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 2,370,211 - BWI76_RS11230 0.63 +0.4 2,370,335 - BWI76_RS11230 0.77 -1.2 2,370,442 + +0.7 2,370,552 + -0.9 2,370,560 - +0.0 2,370,623 + BWI76_RS11235 0.22 +0.6 2,370,904 + +0.7 2,371,030 + -0.0 2,371,166 - -0.2 2,371,571 + BWI76_RS11240 0.35 +1.1 2,371,715 + BWI76_RS11240 0.49 +0.4 2,371,725 + BWI76_RS11240 0.49 +0.1 2,371,762 - BWI76_RS11240 0.53 +0.7 2,371,867 - BWI76_RS11240 0.63 +0.6 2,372,461 - BWI76_RS11245 0.27 +0.6 2,372,599 - BWI76_RS11245 0.44 +0.5 2,372,922 + BWI76_RS11245 0.85 -0.0
Or see this region's nucleotide sequence