Experiment: Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00775 and BWI76_RS00780 are separated by 94 nucleotides BWI76_RS00780 and BWI76_RS00785 are separated by 266 nucleotides BWI76_RS00785 and BWI76_RS00790 are separated by 2 nucleotides
BWI76_RS00775: BWI76_RS00775 - 50S ribosomal protein L31, at 158,919 to 159,134
_RS00775
BWI76_RS00780: BWI76_RS00780 - met repressor, at 159,229 to 159,546
_RS00780
BWI76_RS00785: BWI76_RS00785 - cystathionine gamma-synthase, at 159,813 to 160,973
_RS00785
BWI76_RS00790: BWI76_RS00790 - bifunctional aspartokinase II/homoserine dehydrogenase II, at 160,976 to 163,408
_RS00790
Position (kb) 159
160
161 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 159.493 kb on + strand, within BWI76_RS00780 at 159.623 kb on - strand at 159.654 kb on + strand at 159.654 kb on + strand at 159.803 kb on - strand at 159.889 kb on + strand at 160.023 kb on + strand, within BWI76_RS00785 at 160.223 kb on + strand, within BWI76_RS00785 at 160.262 kb on + strand, within BWI76_RS00785 at 160.277 kb on - strand, within BWI76_RS00785 at 160.337 kb on - strand, within BWI76_RS00785 at 160.423 kb on + strand at 160.526 kb on - strand, within BWI76_RS00785 at 160.546 kb on + strand, within BWI76_RS00785 at 160.575 kb on + strand, within BWI76_RS00785 at 160.575 kb on + strand, within BWI76_RS00785 at 160.607 kb on + strand, within BWI76_RS00785 at 160.770 kb on - strand, within BWI76_RS00785 at 160.906 kb on + strand at 160.906 kb on + strand at 160.906 kb on + strand at 161.001 kb on - strand at 161.015 kb on - strand at 161.424 kb on + strand, within BWI76_RS00790 at 161.587 kb on + strand, within BWI76_RS00790 at 161.635 kb on + strand, within BWI76_RS00790 at 161.852 kb on - strand, within BWI76_RS00790 at 161.866 kb on + strand, within BWI76_RS00790
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 159,493 + BWI76_RS00780 0.83 -0.6 159,623 - +0.2 159,654 + +0.8 159,654 + +0.2 159,803 - -0.0 159,889 + -2.7 160,023 + BWI76_RS00785 0.18 -3.0 160,223 + BWI76_RS00785 0.35 -1.0 160,262 + BWI76_RS00785 0.39 -2.6 160,277 - BWI76_RS00785 0.40 -1.4 160,337 - BWI76_RS00785 0.45 -0.4 160,423 + -1.0 160,526 - BWI76_RS00785 0.61 -1.4 160,546 + BWI76_RS00785 0.63 -4.3 160,575 + BWI76_RS00785 0.66 -3.2 160,575 + BWI76_RS00785 0.66 -2.4 160,607 + BWI76_RS00785 0.68 +1.0 160,770 - BWI76_RS00785 0.82 -2.6 160,906 + -1.8 160,906 + -3.3 160,906 + +1.0 161,001 - +0.5 161,015 - +0.3 161,424 + BWI76_RS00790 0.18 +0.0 161,587 + BWI76_RS00790 0.25 -0.1 161,635 + BWI76_RS00790 0.27 -0.3 161,852 - BWI76_RS00790 0.36 -1.0 161,866 + BWI76_RS00790 0.37 +0.4
Or see this region's nucleotide sequence