Experiment: Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00230 and BWI76_RS00235 are separated by 196 nucleotides BWI76_RS00235 and BWI76_RS00240 overlap by 22 nucleotides BWI76_RS00240 and BWI76_RS00245 are separated by 91 nucleotides BWI76_RS00245 and BWI76_RS00250 are separated by 152 nucleotides
BWI76_RS00230: BWI76_RS00230 - tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA, at 46,681 to 48,570
_RS00230
BWI76_RS00235: BWI76_RS00235 - hypothetical protein, at 48,767 to 48,973
_RS00235
BWI76_RS00240: BWI76_RS00240 - FMN-binding protein MioC, at 48,952 to 49,392
_RS00240
BWI76_RS00245: BWI76_RS00245 - transcriptional regulator AsnC, at 49,484 to 49,942
_RS00245
BWI76_RS00250: BWI76_RS00250 - asparagine synthetase A, at 50,095 to 51,087
_RS00250
Position (kb) 48
49
50 Strain fitness (log2 ratio) -2
-1
0
1
2 at 48.582 kb on - strand at 48.592 kb on - strand at 49.073 kb on + strand, within BWI76_RS00240 at 49.073 kb on + strand, within BWI76_RS00240 at 49.102 kb on + strand, within BWI76_RS00240 at 49.227 kb on - strand, within BWI76_RS00240 at 49.287 kb on + strand at 49.310 kb on + strand at 49.354 kb on + strand at 49.357 kb on - strand at 49.362 kb on - strand at 49.367 kb on - strand at 49.384 kb on - strand at 49.385 kb on - strand at 49.409 kb on + strand at 49.417 kb on - strand at 49.417 kb on - strand at 49.417 kb on - strand at 49.422 kb on - strand at 49.431 kb on - strand at 49.431 kb on - strand at 49.576 kb on + strand, within BWI76_RS00245 at 49.641 kb on + strand, within BWI76_RS00245 at 49.666 kb on - strand, within BWI76_RS00245 at 49.677 kb on + strand, within BWI76_RS00245 at 49.677 kb on + strand, within BWI76_RS00245 at 49.677 kb on + strand, within BWI76_RS00245 at 49.677 kb on + strand, within BWI76_RS00245 at 49.677 kb on + strand, within BWI76_RS00245 at 49.682 kb on + strand, within BWI76_RS00245 at 49.685 kb on - strand, within BWI76_RS00245 at 49.687 kb on + strand, within BWI76_RS00245 at 49.743 kb on + strand, within BWI76_RS00245 at 49.743 kb on + strand, within BWI76_RS00245 at 49.743 kb on + strand, within BWI76_RS00245 at 49.756 kb on + strand, within BWI76_RS00245 at 49.764 kb on - strand, within BWI76_RS00245 at 49.766 kb on + strand, within BWI76_RS00245 at 49.822 kb on + strand, within BWI76_RS00245 at 49.830 kb on + strand, within BWI76_RS00245 at 49.830 kb on - strand, within BWI76_RS00245 at 49.830 kb on - strand, within BWI76_RS00245 at 49.845 kb on - strand, within BWI76_RS00245 at 49.861 kb on + strand, within BWI76_RS00245 at 49.869 kb on - strand, within BWI76_RS00245 at 49.895 kb on + strand, within BWI76_RS00245 at 49.896 kb on + strand, within BWI76_RS00245 at 49.897 kb on + strand at 49.903 kb on - strand at 50.111 kb on + strand at 50.158 kb on - strand at 50.227 kb on + strand, within BWI76_RS00250 at 50.237 kb on - strand, within BWI76_RS00250 at 50.300 kb on - strand, within BWI76_RS00250 at 50.335 kb on - strand, within BWI76_RS00250 at 50.346 kb on + strand, within BWI76_RS00250 at 50.367 kb on + strand, within BWI76_RS00250 at 50.387 kb on - strand, within BWI76_RS00250
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 48,582 - -0.6 48,592 - -0.6 49,073 + BWI76_RS00240 0.27 -1.4 49,073 + BWI76_RS00240 0.27 -1.0 49,102 + BWI76_RS00240 0.34 -1.0 49,227 - BWI76_RS00240 0.62 +1.0 49,287 + +1.0 49,310 + +1.0 49,354 + -0.0 49,357 - +1.4 49,362 - -1.6 49,367 - +1.0 49,384 - +0.5 49,385 - +1.0 49,409 + -1.4 49,417 - -1.0 49,417 - -0.9 49,417 - +0.0 49,422 - -2.5 49,431 - +0.1 49,431 - +1.8 49,576 + BWI76_RS00245 0.20 +0.6 49,641 + BWI76_RS00245 0.34 -0.1 49,666 - BWI76_RS00245 0.40 +0.0 49,677 + BWI76_RS00245 0.42 +0.2 49,677 + BWI76_RS00245 0.42 -1.0 49,677 + BWI76_RS00245 0.42 -0.2 49,677 + BWI76_RS00245 0.42 +0.3 49,677 + BWI76_RS00245 0.42 -1.0 49,682 + BWI76_RS00245 0.43 +0.3 49,685 - BWI76_RS00245 0.44 -0.6 49,687 + BWI76_RS00245 0.44 -0.4 49,743 + BWI76_RS00245 0.56 -0.8 49,743 + BWI76_RS00245 0.56 +1.7 49,743 + BWI76_RS00245 0.56 -0.5 49,756 + BWI76_RS00245 0.59 +1.5 49,764 - BWI76_RS00245 0.61 +0.3 49,766 + BWI76_RS00245 0.61 +0.5 49,822 + BWI76_RS00245 0.74 +1.0 49,830 + BWI76_RS00245 0.75 +0.5 49,830 - BWI76_RS00245 0.75 -0.8 49,830 - BWI76_RS00245 0.75 +0.4 49,845 - BWI76_RS00245 0.79 +0.7 49,861 + BWI76_RS00245 0.82 -2.0 49,869 - BWI76_RS00245 0.84 +0.4 49,895 + BWI76_RS00245 0.90 -0.6 49,896 + BWI76_RS00245 0.90 +0.9 49,897 + +0.3 49,903 - -1.2 50,111 + +0.3 50,158 - +0.1 50,227 + BWI76_RS00250 0.13 +0.2 50,237 - BWI76_RS00250 0.14 +0.5 50,300 - BWI76_RS00250 0.21 -0.2 50,335 - BWI76_RS00250 0.24 +0.0 50,346 + BWI76_RS00250 0.25 +1.0 50,367 + BWI76_RS00250 0.27 +0.6 50,387 - BWI76_RS00250 0.29 +1.6
Or see this region's nucleotide sequence