Experiment: LB anaerobic; ground control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt alaS and recX are separated by 278 nucleotides recX and recA are separated by 113 nucleotides recA and mutS are separated by 380 nucleotides
SO3428: alaS - alanyl-tRNA synthetase (NCBI ptt file), at 3,569,751 to 3,572,375
alaS
SO3429: recX - regulatory protein RecX (NCBI ptt file), at 3,572,654 to 3,573,037
recX
SO3430: recA - recA protein (NCBI ptt file), at 3,573,151 to 3,574,224
recA
SO3431: mutS - DNA mismatch repair protein MutS (NCBI ptt file), at 3,574,605 to 3,577,175
mutS
Position (kb)
3573
3574
3575 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3572.474 kb on + strand at 3572.474 kb on + strand at 3572.482 kb on - strand at 3572.482 kb on - strand at 3572.482 kb on - strand at 3572.482 kb on - strand at 3572.482 kb on - strand at 3572.482 kb on - strand at 3572.482 kb on - strand at 3572.482 kb on - strand at 3572.482 kb on - strand at 3572.532 kb on + strand at 3572.539 kb on - strand at 3572.544 kb on + strand at 3572.584 kb on + strand at 3572.610 kb on + strand at 3572.686 kb on - strand at 3572.784 kb on + strand, within recX at 3572.792 kb on - strand, within recX at 3572.792 kb on - strand, within recX at 3572.895 kb on + strand, within recX at 3572.947 kb on + strand, within recX at 3572.947 kb on + strand, within recX at 3572.983 kb on + strand, within recX at 3572.991 kb on - strand, within recX at 3573.027 kb on - strand at 3573.063 kb on - strand at 3573.149 kb on + strand at 3573.157 kb on - strand at 3573.231 kb on - strand at 3573.319 kb on + strand, within recA at 3573.327 kb on - strand, within recA at 3573.445 kb on - strand, within recA at 3573.521 kb on - strand, within recA at 3573.534 kb on + strand, within recA at 3573.660 kb on - strand, within recA at 3573.670 kb on - strand, within recA at 3573.679 kb on - strand, within recA at 3573.689 kb on + strand, within recA at 3573.823 kb on + strand, within recA at 3573.855 kb on - strand, within recA at 3573.950 kb on + strand, within recA at 3573.955 kb on + strand, within recA at 3573.955 kb on + strand, within recA at 3573.955 kb on + strand, within recA at 3573.955 kb on + strand, within recA at 3573.963 kb on - strand, within recA at 3573.963 kb on - strand, within recA at 3573.963 kb on - strand, within recA at 3574.045 kb on + strand, within recA at 3574.236 kb on + strand at 3574.236 kb on + strand at 3574.236 kb on + strand at 3574.236 kb on + strand at 3574.244 kb on - strand at 3574.254 kb on - strand at 3574.255 kb on - strand at 3574.359 kb on - strand at 3574.415 kb on + strand at 3574.515 kb on - strand at 3574.546 kb on + strand at 3574.642 kb on + strand at 3574.650 kb on - strand at 3574.650 kb on - strand at 3574.672 kb on + strand at 3574.672 kb on + strand at 3574.697 kb on + strand at 3574.722 kb on - strand at 3574.723 kb on - strand at 3574.727 kb on + strand at 3574.773 kb on - strand at 3574.830 kb on - strand at 3574.836 kb on + strand at 3574.939 kb on - strand, within mutS at 3574.956 kb on + strand, within mutS at 3574.961 kb on - strand, within mutS
Per-strain Table
Position Strand Gene LocusTag Fraction LB anaerobic; ground control remove 3,572,474 + +0.3 3,572,474 + -0.4 3,572,482 - -2.1 3,572,482 - +0.4 3,572,482 - -0.4 3,572,482 - +2.6 3,572,482 - +1.5 3,572,482 - +0.4 3,572,482 - -1.4 3,572,482 - +1.5 3,572,482 - +1.2 3,572,532 + -0.1 3,572,539 - -1.1 3,572,544 + +0.6 3,572,584 + +0.6 3,572,610 + +0.1 3,572,686 - +0.3 3,572,784 + recX SO3429 0.34 +1.8 3,572,792 - recX SO3429 0.36 -0.4 3,572,792 - recX SO3429 0.36 -2.4 3,572,895 + recX SO3429 0.63 -0.7 3,572,947 + recX SO3429 0.76 -0.3 3,572,947 + recX SO3429 0.76 +1.9 3,572,983 + recX SO3429 0.86 -0.2 3,572,991 - recX SO3429 0.88 -0.5 3,573,027 - +1.0 3,573,063 - -1.7 3,573,149 + -1.7 3,573,157 - +0.4 3,573,231 - +0.3 3,573,319 + recA SO3430 0.16 -0.5 3,573,327 - recA SO3430 0.16 +0.7 3,573,445 - recA SO3430 0.27 -0.8 3,573,521 - recA SO3430 0.34 -2.7 3,573,534 + recA SO3430 0.36 +0.9 3,573,660 - recA SO3430 0.47 -1.4 3,573,670 - recA SO3430 0.48 -0.7 3,573,679 - recA SO3430 0.49 -2.8 3,573,689 + recA SO3430 0.50 -1.1 3,573,823 + recA SO3430 0.63 -2.1 3,573,855 - recA SO3430 0.66 +0.7 3,573,950 + recA SO3430 0.74 -0.7 3,573,955 + recA SO3430 0.75 -1.4 3,573,955 + recA SO3430 0.75 -2.2 3,573,955 + recA SO3430 0.75 -0.1 3,573,955 + recA SO3430 0.75 -1.1 3,573,963 - recA SO3430 0.76 -0.1 3,573,963 - recA SO3430 0.76 -0.7 3,573,963 - recA SO3430 0.76 +1.9 3,574,045 + recA SO3430 0.83 -1.1 3,574,236 + -0.3 3,574,236 + +0.3 3,574,236 + -0.5 3,574,236 + -1.1 3,574,244 - +0.9 3,574,254 - -0.7 3,574,255 - -0.1 3,574,359 - -2.7 3,574,415 + +0.1 3,574,515 - +0.8 3,574,546 + -1.1 3,574,642 + -0.7 3,574,650 - +0.7 3,574,650 - -0.1 3,574,672 + -0.4 3,574,672 + -1.8 3,574,697 + -3.9 3,574,722 - -1.2 3,574,723 - -0.1 3,574,727 + +0.3 3,574,773 - +1.7 3,574,830 - +1.1 3,574,836 + -3.3 3,574,939 - mutS SO3431 0.13 +0.7 3,574,956 + mutS SO3431 0.14 +0.5 3,574,961 - mutS SO3431 0.14 +0.3
Or see this region's nucleotide sequence