Experiment: LB anaerobic; ground control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt asnS and SO2219 are separated by 195 nucleotides SO2219 and SO2220 are separated by 17 nucleotides SO2220 and pabB are separated by 27 nucleotides
SO2218: asnS - asparaginyl-tRNA synthetase (NCBI ptt file), at 2,332,942 to 2,334,342
asnS
SO2219: SO2219 - hypothetical protein (NCBI ptt file), at 2,334,538 to 2,334,879
SO2219
SO2220: SO2220 - MutT/nudix family protein (NCBI ptt file), at 2,334,897 to 2,335,484
SO2220
SO2221: pabB - para-aminobenzoate synthase, component I (NCBI ptt file), at 2,335,512 to 2,336,915
pabB
Position (kb)
2334
2335
2336 Strain fitness (log2 ratio)
-2
-1
0
1 at 2334.453 kb on - strand at 2334.472 kb on - strand at 2334.493 kb on + strand at 2334.638 kb on - strand, within SO2219 at 2334.644 kb on - strand, within SO2219 at 2334.685 kb on - strand, within SO2219 at 2334.789 kb on - strand, within SO2219 at 2334.913 kb on + strand at 2334.918 kb on + strand at 2335.000 kb on + strand, within SO2220 at 2335.013 kb on - strand, within SO2220 at 2335.257 kb on - strand, within SO2220 at 2335.378 kb on + strand, within SO2220 at 2335.438 kb on + strand at 2335.438 kb on + strand at 2335.446 kb on - strand at 2335.618 kb on + strand at 2335.689 kb on + strand, within pabB at 2335.899 kb on - strand, within pabB at 2336.217 kb on - strand, within pabB at 2336.321 kb on + strand, within pabB
Per-strain Table
Position Strand Gene LocusTag Fraction LB anaerobic; ground control remove 2,334,453 - -0.8 2,334,472 - -0.1 2,334,493 + +0.4 2,334,638 - SO2219 0.29 -0.5 2,334,644 - SO2219 0.31 +1.3 2,334,685 - SO2219 0.43 -0.1 2,334,789 - SO2219 0.73 -0.3 2,334,913 + +0.8 2,334,918 + -2.1 2,335,000 + SO2220 0.18 -0.4 2,335,013 - SO2220 0.20 -1.5 2,335,257 - SO2220 0.61 -0.2 2,335,378 + SO2220 0.82 -2.5 2,335,438 + -0.3 2,335,438 + -1.8 2,335,446 - +0.6 2,335,618 + -0.9 2,335,689 + pabB SO2221 0.13 -0.2 2,335,899 - pabB SO2221 0.28 -0.5 2,336,217 - pabB SO2221 0.50 +0.9 2,336,321 + pabB SO2221 0.58 -0.5
Or see this region's nucleotide sequence