Experiment: Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08375 and BWI76_RS08380 are separated by 15 nucleotides BWI76_RS08380 and BWI76_RS08385 are separated by 45 nucleotides BWI76_RS08385 and BWI76_RS08390 are separated by 437 nucleotides BWI76_RS08390 and BWI76_RS08395 overlap by 17 nucleotides
BWI76_RS08375: BWI76_RS08375 - pyroglutamyl-peptidase I, at 1,772,181 to 1,772,825
_RS08375
BWI76_RS08380: BWI76_RS08380 - endonuclease VIII, at 1,772,841 to 1,773,632
_RS08380
BWI76_RS08385: BWI76_RS08385 - citrate (Si)-synthase, at 1,773,678 to 1,774,961
_RS08385
BWI76_RS08390: BWI76_RS08390 - hypothetical protein, at 1,775,399 to 1,775,617
_RS08390
BWI76_RS08395: BWI76_RS08395 - succinate dehydrogenase cytochrome b556 large subunit, at 1,775,601 to 1,776,005
_RS08395
Position (kb) 1773
1774
1775 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 1772.904 kb on + strand at 1772.912 kb on - strand at 1773.069 kb on + strand, within BWI76_RS08380 at 1773.220 kb on + strand, within BWI76_RS08380 at 1773.794 kb on + strand at 1773.862 kb on - strand, within BWI76_RS08385 at 1774.346 kb on + strand, within BWI76_RS08385 at 1774.604 kb on - strand, within BWI76_RS08385 at 1775.091 kb on + strand at 1775.099 kb on - strand at 1775.114 kb on + strand at 1775.183 kb on + strand at 1775.196 kb on - strand at 1775.207 kb on + strand at 1775.215 kb on + strand at 1775.450 kb on + strand, within BWI76_RS08390 at 1775.461 kb on - strand, within BWI76_RS08390 at 1775.658 kb on - strand at 1775.929 kb on - strand, within BWI76_RS08395
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 1,772,904 + -1.7 1,772,912 - -0.5 1,773,069 + BWI76_RS08380 0.29 +1.5 1,773,220 + BWI76_RS08380 0.48 -0.7 1,773,794 + +0.9 1,773,862 - BWI76_RS08385 0.14 -2.1 1,774,346 + BWI76_RS08385 0.52 -5.0 1,774,604 - BWI76_RS08385 0.72 -1.7 1,775,091 + +0.9 1,775,099 - -0.4 1,775,114 + -1.9 1,775,183 + -1.4 1,775,196 - -0.5 1,775,207 + -0.6 1,775,215 + -2.0 1,775,450 + BWI76_RS08390 0.23 +0.4 1,775,461 - BWI76_RS08390 0.28 -1.4 1,775,658 - +0.3 1,775,929 - BWI76_RS08395 0.81 -0.1
Or see this region's nucleotide sequence