Experiment: Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04830 and BWI76_RS04835 overlap by 11 nucleotides BWI76_RS04835 and BWI76_RS04840 are separated by 10 nucleotides BWI76_RS04840 and BWI76_RS04845 are separated by 183 nucleotides
BWI76_RS04830: BWI76_RS04830 - type IV pilin biogenesis protein, at 1,008,810 to 1,010,015
_RS04830
BWI76_RS04835: BWI76_RS04835 - type II secretion system protein GspE, at 1,010,005 to 1,011,390
_RS04835
BWI76_RS04840: BWI76_RS04840 - prepilin peptidase-dependent pilin, at 1,011,401 to 1,011,832
_RS04840
BWI76_RS04845: BWI76_RS04845 - nicotinate-nucleotide pyrophosphorylase, at 1,012,016 to 1,012,909
_RS04845
Position (kb)
1010
1011
1012 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1009.028 kb on + strand, within BWI76_RS04830 at 1009.028 kb on + strand, within BWI76_RS04830 at 1009.267 kb on + strand, within BWI76_RS04830 at 1009.418 kb on + strand, within BWI76_RS04830 at 1009.480 kb on + strand, within BWI76_RS04830 at 1009.486 kb on + strand, within BWI76_RS04830 at 1009.562 kb on - strand, within BWI76_RS04830 at 1009.712 kb on + strand, within BWI76_RS04830 at 1009.714 kb on + strand, within BWI76_RS04830 at 1009.777 kb on + strand, within BWI76_RS04830 at 1009.868 kb on - strand, within BWI76_RS04830 at 1010.247 kb on - strand, within BWI76_RS04835 at 1010.279 kb on - strand, within BWI76_RS04835 at 1010.289 kb on - strand, within BWI76_RS04835 at 1010.591 kb on + strand, within BWI76_RS04835 at 1010.797 kb on - strand, within BWI76_RS04835 at 1010.836 kb on - strand, within BWI76_RS04835 at 1011.154 kb on + strand, within BWI76_RS04835 at 1011.270 kb on + strand at 1011.506 kb on - strand, within BWI76_RS04840 at 1011.553 kb on + strand, within BWI76_RS04840 at 1011.670 kb on + strand, within BWI76_RS04840 at 1011.698 kb on + strand, within BWI76_RS04840 at 1011.706 kb on - strand, within BWI76_RS04840 at 1011.808 kb on + strand at 1011.808 kb on + strand at 1012.036 kb on + strand at 1012.100 kb on - strand at 1012.194 kb on - strand, within BWI76_RS04845
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 1,009,028 + BWI76_RS04830 0.18 +0.7 1,009,028 + BWI76_RS04830 0.18 +0.9 1,009,267 + BWI76_RS04830 0.38 +0.6 1,009,418 + BWI76_RS04830 0.50 -0.1 1,009,480 + BWI76_RS04830 0.56 +0.2 1,009,486 + BWI76_RS04830 0.56 +1.9 1,009,562 - BWI76_RS04830 0.62 -0.7 1,009,712 + BWI76_RS04830 0.75 +1.1 1,009,714 + BWI76_RS04830 0.75 -0.1 1,009,777 + BWI76_RS04830 0.80 +0.7 1,009,868 - BWI76_RS04830 0.88 -0.3 1,010,247 - BWI76_RS04835 0.17 -1.1 1,010,279 - BWI76_RS04835 0.20 -1.4 1,010,289 - BWI76_RS04835 0.20 -0.8 1,010,591 + BWI76_RS04835 0.42 +0.1 1,010,797 - BWI76_RS04835 0.57 +0.5 1,010,836 - BWI76_RS04835 0.60 -1.1 1,011,154 + BWI76_RS04835 0.83 -0.1 1,011,270 + -0.7 1,011,506 - BWI76_RS04840 0.24 +0.6 1,011,553 + BWI76_RS04840 0.35 +0.4 1,011,670 + BWI76_RS04840 0.62 +1.3 1,011,698 + BWI76_RS04840 0.69 +1.6 1,011,706 - BWI76_RS04840 0.71 -1.1 1,011,808 + -2.8 1,011,808 + +0.2 1,012,036 + -3.5 1,012,100 - -4.0 1,012,194 - BWI76_RS04845 0.20 -2.8
Or see this region's nucleotide sequence