Experiment: LB anaerobic; ground control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt phnA and SO1582 are separated by 173 nucleotides SO1582 and SO1583 are separated by 69 nucleotides SO1583 and SO1585 are separated by 332 nucleotides SO1585 and SO1586 are separated by 20 nucleotides
SO1581: phnA - phnA protein (NCBI ptt file), at 1,658,982 to 1,659,545
phnA
SO1582: SO1582 - transcriptional regulator, MarR family (NCBI ptt file), at 1,659,719 to 1,660,258
SO1582
SO1583: SO1583 - conserved hypothetical protein (NCBI ptt file), at 1,660,328 to 1,661,341
SO1583
SO1585: SO1585 - fumarylacetoacetate hydrolase family protein (NCBI ptt file), at 1,661,674 to 1,662,300
SO1585
SO1586: SO1586 - hypothetical Cyclic nucleotide-binding domain protein (NCBI ptt file), at 1,662,321 to 1,662,923
SO1586
Position (kb)
1660
1661
1662 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1659.342 kb on + strand, within phnA at 1659.472 kb on + strand, within phnA at 1659.567 kb on + strand at 1659.737 kb on - strand at 1659.764 kb on - strand at 1659.842 kb on + strand, within SO1582 at 1659.912 kb on + strand, within SO1582 at 1659.964 kb on + strand, within SO1582 at 1659.972 kb on - strand, within SO1582 at 1660.077 kb on + strand, within SO1582 at 1660.127 kb on + strand, within SO1582 at 1660.135 kb on - strand, within SO1582 at 1660.284 kb on - strand at 1660.410 kb on - strand at 1660.414 kb on + strand at 1660.414 kb on + strand at 1660.414 kb on + strand at 1660.414 kb on + strand at 1660.422 kb on - strand at 1660.422 kb on - strand at 1660.422 kb on - strand at 1660.422 kb on - strand at 1660.529 kb on - strand, within SO1583 at 1660.737 kb on - strand, within SO1583 at 1660.896 kb on + strand, within SO1583 at 1660.958 kb on - strand, within SO1583 at 1660.978 kb on + strand, within SO1583 at 1660.984 kb on - strand, within SO1583 at 1660.993 kb on - strand, within SO1583 at 1661.110 kb on + strand, within SO1583 at 1661.110 kb on + strand, within SO1583 at 1661.137 kb on + strand, within SO1583 at 1661.138 kb on + strand, within SO1583 at 1661.181 kb on - strand, within SO1583 at 1661.189 kb on - strand, within SO1583 at 1661.255 kb on + strand at 1661.275 kb on + strand at 1661.275 kb on + strand at 1661.275 kb on + strand at 1661.275 kb on + strand at 1661.652 kb on - strand at 1661.652 kb on - strand at 1661.874 kb on + strand, within SO1585 at 1661.933 kb on + strand, within SO1585 at 1661.951 kb on + strand, within SO1585 at 1661.997 kb on - strand, within SO1585 at 1662.110 kb on + strand, within SO1585 at 1662.110 kb on + strand, within SO1585 at 1662.154 kb on + strand, within SO1585 at 1662.209 kb on - strand, within SO1585 at 1662.235 kb on + strand, within SO1585 at 1662.321 kb on + strand at 1662.337 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB anaerobic; ground control remove 1,659,342 + phnA SO1581 0.64 -0.1 1,659,472 + phnA SO1581 0.87 +1.1 1,659,567 + +1.3 1,659,737 - +0.3 1,659,764 - -1.6 1,659,842 + SO1582 0.23 +1.5 1,659,912 + SO1582 0.36 +1.9 1,659,964 + SO1582 0.45 +0.7 1,659,972 - SO1582 0.47 +0.5 1,660,077 + SO1582 0.66 +0.4 1,660,127 + SO1582 0.76 +0.9 1,660,135 - SO1582 0.77 -0.1 1,660,284 - -2.8 1,660,410 - +1.4 1,660,414 + +1.2 1,660,414 + -2.2 1,660,414 + +0.4 1,660,414 + -0.2 1,660,422 - -0.1 1,660,422 - +1.3 1,660,422 - -0.6 1,660,422 - +0.4 1,660,529 - SO1583 0.20 +0.2 1,660,737 - SO1583 0.40 +0.6 1,660,896 + SO1583 0.56 +2.0 1,660,958 - SO1583 0.62 +0.8 1,660,978 + SO1583 0.64 -1.1 1,660,984 - SO1583 0.65 -0.3 1,660,993 - SO1583 0.66 +1.0 1,661,110 + SO1583 0.77 +0.4 1,661,110 + SO1583 0.77 +2.8 1,661,137 + SO1583 0.80 +0.9 1,661,138 + SO1583 0.80 +0.2 1,661,181 - SO1583 0.84 +0.5 1,661,189 - SO1583 0.85 -1.8 1,661,255 + -0.7 1,661,275 + +0.7 1,661,275 + +0.9 1,661,275 + +0.2 1,661,275 + -1.4 1,661,652 - -0.9 1,661,652 - +0.4 1,661,874 + SO1585 0.32 -0.6 1,661,933 + SO1585 0.41 +0.4 1,661,951 + SO1585 0.44 -0.7 1,661,997 - SO1585 0.52 +0.6 1,662,110 + SO1585 0.70 -1.6 1,662,110 + SO1585 0.70 -1.5 1,662,154 + SO1585 0.77 -0.1 1,662,209 - SO1585 0.85 -1.7 1,662,235 + SO1585 0.89 +0.9 1,662,321 + -1.4 1,662,337 + +2.4
Or see this region's nucleotide sequence