Experiment: Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27315 and BWI76_RS27320 overlap by 1 nucleotides BWI76_RS27320 and BWI76_RS27325 overlap by 4 nucleotides BWI76_RS27325 and BWI76_RS27330 are separated by 100 nucleotides BWI76_RS27330 and BWI76_RS27335 are separated by 63 nucleotides
BWI76_RS27315: BWI76_RS27315 - 3-deoxy-D-manno-octulosonic acid transferase, at 5,669,571 to 5,670,845
_RS27315
BWI76_RS27320: BWI76_RS27320 - glucosyl transferase, at 5,670,845 to 5,671,621
_RS27320
BWI76_RS27325: BWI76_RS27325 - pantetheine-phosphate adenylyltransferase, at 5,671,618 to 5,672,094
_RS27325
BWI76_RS27330: BWI76_RS27330 - DNA-formamidopyrimidine glycosylase, at 5,672,195 to 5,673,004
_RS27330
BWI76_RS27335: BWI76_RS27335 - hypothetical protein, at 5,673,068 to 5,675,404
_RS27335
Position (kb) 5671
5672
5673 Strain fitness (log2 ratio) -1
0
1 at 5672.233 kb on + strand at 5672.241 kb on - strand at 5672.371 kb on + strand, within BWI76_RS27330 at 5672.540 kb on - strand, within BWI76_RS27330 at 5672.586 kb on + strand, within BWI76_RS27330 at 5672.688 kb on + strand, within BWI76_RS27330 at 5672.752 kb on + strand, within BWI76_RS27330 at 5672.911 kb on - strand, within BWI76_RS27330 at 5672.967 kb on - strand at 5673.055 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 5,672,233 + +0.1 5,672,241 - +0.4 5,672,371 + BWI76_RS27330 0.22 -1.1 5,672,540 - BWI76_RS27330 0.43 +0.9 5,672,586 + BWI76_RS27330 0.48 +1.4 5,672,688 + BWI76_RS27330 0.61 +0.3 5,672,752 + BWI76_RS27330 0.69 -1.4 5,672,911 - BWI76_RS27330 0.88 +0.0 5,672,967 - -1.6 5,673,055 - +0.5
Or see this region's nucleotide sequence