Experiment: Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22150 and BWI76_RS22155 are separated by 60 nucleotides BWI76_RS22155 and BWI76_RS22160 are separated by 4 nucleotides BWI76_RS22160 and BWI76_RS22165 are separated by 323 nucleotides BWI76_RS22165 and BWI76_RS22170 are separated by 238 nucleotides BWI76_RS22170 and BWI76_RS22175 are separated by 130 nucleotides BWI76_RS22175 and BWI76_RS22180 are separated by 70 nucleotides
BWI76_RS22150: BWI76_RS22150 - tRNA-Arg, at 4,621,647 to 4,621,723
_RS22150
BWI76_RS22155: BWI76_RS22155 - tRNA-Arg, at 4,621,784 to 4,621,860
_RS22155
BWI76_RS22160: BWI76_RS22160 - tRNA-Ser, at 4,621,865 to 4,621,957
_RS22160
BWI76_RS22165: BWI76_RS22165 - carbon storage regulator, at 4,622,281 to 4,622,466
_RS22165
BWI76_RS22170: BWI76_RS22170 - alanine--tRNA ligase, at 4,622,705 to 4,625,332
_RS22170
BWI76_RS22175: BWI76_RS22175 - recombination regulator RecX, at 4,625,463 to 4,625,963
_RS22175
BWI76_RS22180: BWI76_RS22180 - recombinase RecA, at 4,626,034 to 4,627,092
_RS22180
Position (kb) 4622
4623
4624
4625
4626 Strain fitness (log2 ratio) -1
0
1 at 4622.581 kb on - strand at 4622.587 kb on - strand at 4625.512 kb on + strand at 4625.514 kb on - strand, within BWI76_RS22175 at 4625.936 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 4,622,581 - +1.1 4,622,587 - -0.2 4,625,512 + +0.2 4,625,514 - BWI76_RS22175 0.10 -1.6 4,625,936 - -0.1
Or see this region's nucleotide sequence