Experiment: Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04835 and BWI76_RS04840 are separated by 10 nucleotides BWI76_RS04840 and BWI76_RS04845 are separated by 183 nucleotides BWI76_RS04845 and BWI76_RS04850 are separated by 194 nucleotides BWI76_RS04850 and BWI76_RS04855 are separated by 9 nucleotides
BWI76_RS04835: BWI76_RS04835 - type II secretion system protein GspE, at 1,010,005 to 1,011,390
_RS04835
BWI76_RS04840: BWI76_RS04840 - prepilin peptidase-dependent pilin, at 1,011,401 to 1,011,832
_RS04840
BWI76_RS04845: BWI76_RS04845 - nicotinate-nucleotide pyrophosphorylase, at 1,012,016 to 1,012,909
_RS04845
BWI76_RS04850: BWI76_RS04850 - N-acetylmuramoyl-L-alanine amidase, at 1,013,104 to 1,013,655
_RS04850
BWI76_RS04855: BWI76_RS04855 - regulatory protein AmpE, at 1,013,665 to 1,014,519
_RS04855
Position (kb) 1012
1013 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1011.154 kb on + strand, within BWI76_RS04835 at 1011.270 kb on + strand at 1011.506 kb on - strand, within BWI76_RS04840 at 1011.553 kb on + strand, within BWI76_RS04840 at 1011.670 kb on + strand, within BWI76_RS04840 at 1011.698 kb on + strand, within BWI76_RS04840 at 1011.706 kb on - strand, within BWI76_RS04840 at 1011.808 kb on + strand at 1011.808 kb on + strand at 1012.036 kb on + strand at 1012.100 kb on - strand at 1012.194 kb on - strand, within BWI76_RS04845 at 1012.418 kb on + strand, within BWI76_RS04845 at 1012.426 kb on - strand, within BWI76_RS04845 at 1012.802 kb on + strand, within BWI76_RS04845 at 1012.802 kb on + strand, within BWI76_RS04845 at 1012.810 kb on - strand, within BWI76_RS04845 at 1013.094 kb on - strand at 1013.256 kb on + strand, within BWI76_RS04850 at 1013.368 kb on + strand, within BWI76_RS04850 at 1013.398 kb on + strand, within BWI76_RS04850 at 1013.472 kb on + strand, within BWI76_RS04850 at 1013.514 kb on - strand at 1013.613 kb on - strand at 1013.675 kb on + strand at 1013.681 kb on - strand at 1013.708 kb on + strand at 1013.716 kb on - strand at 1013.716 kb on - strand at 1013.760 kb on - strand, within BWI76_RS04855 at 1013.784 kb on + strand, within BWI76_RS04855 at 1013.844 kb on + strand, within BWI76_RS04855 at 1013.904 kb on + strand, within BWI76_RS04855 at 1013.904 kb on - strand, within BWI76_RS04855
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 1,011,154 + BWI76_RS04835 0.83 -0.3 1,011,270 + -0.6 1,011,506 - BWI76_RS04840 0.24 +0.4 1,011,553 + BWI76_RS04840 0.35 +0.3 1,011,670 + BWI76_RS04840 0.62 +1.3 1,011,698 + BWI76_RS04840 0.69 +1.3 1,011,706 - BWI76_RS04840 0.71 +0.7 1,011,808 + -2.7 1,011,808 + +0.1 1,012,036 + -2.7 1,012,100 - -1.9 1,012,194 - BWI76_RS04845 0.20 -2.6 1,012,418 + BWI76_RS04845 0.45 -1.1 1,012,426 - BWI76_RS04845 0.46 -2.5 1,012,802 + BWI76_RS04845 0.88 -3.3 1,012,802 + BWI76_RS04845 0.88 -2.7 1,012,810 - BWI76_RS04845 0.89 -2.4 1,013,094 - +0.3 1,013,256 + BWI76_RS04850 0.28 +0.6 1,013,368 + BWI76_RS04850 0.48 -1.1 1,013,398 + BWI76_RS04850 0.53 +1.7 1,013,472 + BWI76_RS04850 0.67 +0.2 1,013,514 - -0.0 1,013,613 - +0.1 1,013,675 + +0.7 1,013,681 - +0.8 1,013,708 + +0.9 1,013,716 - +0.8 1,013,716 - +0.5 1,013,760 - BWI76_RS04855 0.11 +0.1 1,013,784 + BWI76_RS04855 0.14 -0.0 1,013,844 + BWI76_RS04855 0.21 -0.0 1,013,904 + BWI76_RS04855 0.28 +0.1 1,013,904 - BWI76_RS04855 0.28 -1.6
Or see this region's nucleotide sequence