Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01855

Experiment: Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01850 and BWI76_RS01855 are separated by 188 nucleotidesBWI76_RS01855 and BWI76_RS01860 are separated by 12 nucleotidesBWI76_RS01860 and BWI76_RS01865 are separated by 98 nucleotides BWI76_RS01850: BWI76_RS01850 - maltose operon protein MalM, at 377,462 to 378,394 _RS01850 BWI76_RS01855: BWI76_RS01855 - chorismate lyase, at 378,583 to 379,080 _RS01855 BWI76_RS01860: BWI76_RS01860 - 4-hydroxybenzoate octaprenyltransferase, at 379,093 to 379,962 _RS01860 BWI76_RS01865: BWI76_RS01865 - glycerol-3-phosphate 1-O-acyltransferase, at 380,061 to 382,484 _RS01865 Position (kb) 378 379 380Strain fitness (log2 ratio) -2 -1 0 1at 377.608 kb on + strand, within BWI76_RS01850at 377.782 kb on - strand, within BWI76_RS01850at 377.998 kb on + strand, within BWI76_RS01850at 377.998 kb on + strand, within BWI76_RS01850at 377.998 kb on + strand, within BWI76_RS01850at 378.006 kb on - strand, within BWI76_RS01850at 378.093 kb on + strand, within BWI76_RS01850at 378.108 kb on + strand, within BWI76_RS01850at 378.383 kb on - strandat 378.398 kb on + strandat 378.427 kb on + strandat 378.427 kb on + strandat 378.435 kb on - strandat 378.887 kb on + strand, within BWI76_RS01855at 378.895 kb on - strand, within BWI76_RS01855at 379.069 kb on + strandat 380.039 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
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377,608 + BWI76_RS01850 0.16 -0.4
377,782 - BWI76_RS01850 0.34 +1.4
377,998 + BWI76_RS01850 0.57 +0.1
377,998 + BWI76_RS01850 0.57 +0.4
377,998 + BWI76_RS01850 0.57 -0.1
378,006 - BWI76_RS01850 0.58 -0.1
378,093 + BWI76_RS01850 0.68 -0.4
378,108 + BWI76_RS01850 0.69 -0.2
378,383 - -2.6
378,398 + -0.6
378,427 + -1.3
378,427 + +1.3
378,435 - -2.3
378,887 + BWI76_RS01855 0.61 +0.7
378,895 - BWI76_RS01855 0.63 +1.7
379,069 + +0.7
380,039 + -0.2

Or see this region's nucleotide sequence