Strain Fitness in Shewanella oneidensis MR-1 around SO3257

Experiment: Fumarate electron acceptor; ground control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntflgM and flgN are separated by 9 nucleotidesflgN and SO3256 are separated by 117 nucleotidesSO3256 and SO3257 overlap by 4 nucleotidesSO3257 and SO3258 are separated by 273 nucleotides SO3254: flgM - negative regulator of flagellin synthesis FlgM (NCBI ptt file), at 3,391,687 to 3,392,007 flgM SO3255: flgN - flagellar biosynthetic protein FlgN (NCBI ptt file), at 3,392,017 to 3,392,442 flgN SO3256: SO3256 - conserved hypothetical protein (NCBI ptt file), at 3,392,560 to 3,393,009 SO3256 SO3257: SO3257 - conserved hypothetical protein (NCBI ptt file), at 3,393,006 to 3,393,638 SO3257 SO3258: SO3258 - conserved hypothetical protein (NCBI ptt file), at 3,393,912 to 3,395,087 SO3258 Position (kb) 3393 3394Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 3392.042 kb on + strandat 3392.253 kb on + strand, within flgNat 3392.266 kb on - strand, within flgNat 3392.266 kb on - strand, within flgNat 3392.336 kb on + strand, within flgNat 3392.341 kb on + strand, within flgNat 3392.349 kb on - strand, within flgNat 3392.394 kb on + strand, within flgNat 3392.529 kb on - strandat 3392.530 kb on + strandat 3392.540 kb on + strandat 3392.548 kb on - strandat 3392.548 kb on - strandat 3392.548 kb on - strandat 3392.685 kb on + strand, within SO3256at 3392.742 kb on + strand, within SO3256at 3392.891 kb on - strand, within SO3256at 3392.904 kb on + strand, within SO3256at 3392.912 kb on - strand, within SO3256at 3392.912 kb on - strand, within SO3256at 3392.912 kb on - strand, within SO3256at 3393.089 kb on + strand, within SO3257at 3393.101 kb on + strand, within SO3257at 3393.160 kb on - strand, within SO3257at 3393.331 kb on - strand, within SO3257at 3393.636 kb on - strandat 3393.640 kb on + strandat 3393.648 kb on - strandat 3393.677 kb on - strandat 3393.717 kb on - strandat 3393.758 kb on - strandat 3393.788 kb on - strandat 3393.843 kb on + strandat 3393.861 kb on - strandat 3393.872 kb on - strandat 3393.949 kb on + strandat 3393.957 kb on - strandat 3393.959 kb on + strandat 3393.971 kb on + strandat 3393.979 kb on - strandat 3394.036 kb on - strand, within SO3258at 3394.046 kb on - strand, within SO3258at 3394.085 kb on + strand, within SO3258at 3394.086 kb on - strand, within SO3258at 3394.150 kb on + strand, within SO3258at 3394.244 kb on + strand, within SO3258at 3394.245 kb on - strand, within SO3258at 3394.283 kb on + strand, within SO3258at 3394.283 kb on + strand, within SO3258at 3394.311 kb on + strand, within SO3258at 3394.332 kb on + strand, within SO3258at 3394.332 kb on + strand, within SO3258at 3394.340 kb on - strand, within SO3258at 3394.340 kb on - strand, within SO3258at 3394.340 kb on - strand, within SO3258at 3394.340 kb on - strand, within SO3258at 3394.424 kb on - strand, within SO3258at 3394.445 kb on + strand, within SO3258at 3394.495 kb on - strand, within SO3258at 3394.561 kb on + strand, within SO3258at 3394.571 kb on + strand, within SO3258

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Per-strain Table

Position Strand Gene LocusTag Fraction Fumarate electron acceptor; ground control
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3,392,042 + +0.9
3,392,253 + flgN SO3255 0.55 -1.1
3,392,266 - flgN SO3255 0.58 -3.1
3,392,266 - flgN SO3255 0.58 -0.2
3,392,336 + flgN SO3255 0.75 -1.1
3,392,341 + flgN SO3255 0.76 -1.3
3,392,349 - flgN SO3255 0.78 -1.6
3,392,394 + flgN SO3255 0.88 -0.6
3,392,529 - -0.5
3,392,530 + -0.3
3,392,540 + +0.8
3,392,548 - -1.9
3,392,548 - -2.8
3,392,548 - +1.3
3,392,685 + SO3256 0.28 -2.8
3,392,742 + SO3256 0.40 +0.2
3,392,891 - SO3256 0.74 -1.3
3,392,904 + SO3256 0.76 -0.8
3,392,912 - SO3256 0.78 -0.5
3,392,912 - SO3256 0.78 +0.1
3,392,912 - SO3256 0.78 -1.3
3,393,089 + SO3257 0.13 -2.1
3,393,101 + SO3257 0.15 -2.1
3,393,160 - SO3257 0.24 -0.9
3,393,331 - SO3257 0.51 -2.2
3,393,636 - +0.8
3,393,640 + +1.3
3,393,648 - -2.8
3,393,677 - +0.0
3,393,717 - -0.5
3,393,758 - -0.3
3,393,788 - +0.4
3,393,843 + -2.2
3,393,861 - -1.3
3,393,872 - -0.3
3,393,949 + -3.5
3,393,957 - -3.3
3,393,959 + -3.6
3,393,971 + -2.2
3,393,979 - -3.6
3,394,036 - SO3258 0.11 -1.3
3,394,046 - SO3258 0.11 -2.2
3,394,085 + SO3258 0.15 -3.1
3,394,086 - SO3258 0.15 -2.5
3,394,150 + SO3258 0.20 -4.4
3,394,244 + SO3258 0.28 +0.9
3,394,245 - SO3258 0.28 -5.0
3,394,283 + SO3258 0.32 -2.3
3,394,283 + SO3258 0.32 -3.2
3,394,311 + SO3258 0.34 +1.1
3,394,332 + SO3258 0.36 -2.8
3,394,332 + SO3258 0.36 -2.3
3,394,340 - SO3258 0.36 -0.5
3,394,340 - SO3258 0.36 -0.2
3,394,340 - SO3258 0.36 -0.8
3,394,340 - SO3258 0.36 -3.6
3,394,424 - SO3258 0.44 -2.8
3,394,445 + SO3258 0.45 -0.7
3,394,495 - SO3258 0.50 -2.3
3,394,561 + SO3258 0.55 -2.9
3,394,571 + SO3258 0.56 -1.8

Or see this region's nucleotide sequence