Strain Fitness in Shewanella oneidensis MR-1 around SO3234

Experiment: Fumarate electron acceptor; ground control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntflrA and fliS are separated by 294 nucleotidesfliS and SO3234 are separated by 6 nucleotidesSO3234 and fliD are separated by 28 nucleotides SO3232: flrA - flagellar regulatory protein A (NCBI ptt file), at 3,371,358 to 3,372,791 flrA SO3233: fliS - flagellar protein FliS (NCBI ptt file), at 3,373,086 to 3,373,496 fliS SO3234: SO3234 - hypothetical protein (NCBI ptt file), at 3,373,503 to 3,373,823 SO3234 SO3235: fliD - flagellar hook-associated protein FliD (NCBI ptt file), at 3,373,852 to 3,375,222 fliD Position (kb) 3373 3374Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 3372.524 kb on + strand, within flrAat 3372.586 kb on - strand, within flrAat 3372.620 kb on + strand, within flrAat 3372.744 kb on - strandat 3372.788 kb on + strandat 3372.884 kb on - strandat 3372.901 kb on - strandat 3372.917 kb on + strandat 3373.116 kb on + strandat 3373.116 kb on + strandat 3373.116 kb on + strandat 3373.136 kb on + strand, within fliSat 3373.145 kb on - strand, within fliSat 3373.156 kb on - strand, within fliSat 3373.361 kb on + strand, within fliSat 3373.370 kb on + strand, within fliSat 3373.377 kb on + strand, within fliSat 3373.377 kb on + strand, within fliSat 3373.385 kb on - strand, within fliSat 3373.385 kb on - strand, within fliSat 3373.385 kb on - strand, within fliSat 3373.389 kb on + strand, within fliSat 3373.389 kb on + strand, within fliSat 3373.397 kb on - strand, within fliSat 3373.400 kb on - strand, within fliSat 3373.426 kb on - strand, within fliSat 3373.500 kb on + strandat 3373.500 kb on - strandat 3373.539 kb on - strand, within SO3234at 3373.624 kb on + strand, within SO3234at 3373.684 kb on - strand, within SO3234at 3373.711 kb on - strand, within SO3234at 3373.711 kb on - strand, within SO3234at 3373.733 kb on + strand, within SO3234at 3373.749 kb on + strand, within SO3234at 3373.775 kb on + strand, within SO3234at 3373.775 kb on + strand, within SO3234at 3373.783 kb on - strand, within SO3234at 3373.801 kb on - strandat 3374.020 kb on + strand, within fliDat 3374.028 kb on - strand, within fliDat 3374.036 kb on - strand, within fliDat 3374.178 kb on + strand, within fliDat 3374.323 kb on + strand, within fliDat 3374.331 kb on - strand, within fliDat 3374.371 kb on - strand, within fliDat 3374.468 kb on + strand, within fliDat 3374.642 kb on - strand, within fliDat 3374.642 kb on - strand, within fliDat 3374.698 kb on + strand, within fliDat 3374.740 kb on + strand, within fliD

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Per-strain Table

Position Strand Gene LocusTag Fraction Fumarate electron acceptor; ground control
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3,372,524 + flrA SO3232 0.81 -3.9
3,372,586 - flrA SO3232 0.86 +1.4
3,372,620 + flrA SO3232 0.88 +1.5
3,372,744 - -0.5
3,372,788 + +1.9
3,372,884 - +0.9
3,372,901 - -1.4
3,372,917 + +1.9
3,373,116 + +2.6
3,373,116 + +0.7
3,373,116 + +0.6
3,373,136 + fliS SO3233 0.12 +1.4
3,373,145 - fliS SO3233 0.14 +3.8
3,373,156 - fliS SO3233 0.17 -1.4
3,373,361 + fliS SO3233 0.67 -2.7
3,373,370 + fliS SO3233 0.69 -1.9
3,373,377 + fliS SO3233 0.71 -0.4
3,373,377 + fliS SO3233 0.71 +1.0
3,373,385 - fliS SO3233 0.73 -0.4
3,373,385 - fliS SO3233 0.73 -2.9
3,373,385 - fliS SO3233 0.73 -1.4
3,373,389 + fliS SO3233 0.74 +1.4
3,373,389 + fliS SO3233 0.74 -1.9
3,373,397 - fliS SO3233 0.76 -2.5
3,373,400 - fliS SO3233 0.76 +1.6
3,373,426 - fliS SO3233 0.83 -3.6
3,373,500 + -3.7
3,373,500 - +0.2
3,373,539 - SO3234 0.11 -0.2
3,373,624 + SO3234 0.38 -1.3
3,373,684 - SO3234 0.56 -3.7
3,373,711 - SO3234 0.65 -2.8
3,373,711 - SO3234 0.65 -2.4
3,373,733 + SO3234 0.72 -3.2
3,373,749 + SO3234 0.77 -1.1
3,373,775 + SO3234 0.85 -0.7
3,373,775 + SO3234 0.85 -3.3
3,373,783 - SO3234 0.87 -0.6
3,373,801 - +0.3
3,374,020 + fliD SO3235 0.12 -1.8
3,374,028 - fliD SO3235 0.13 -0.5
3,374,036 - fliD SO3235 0.13 -1.7
3,374,178 + fliD SO3235 0.24 -2.2
3,374,323 + fliD SO3235 0.34 +2.7
3,374,331 - fliD SO3235 0.35 +0.4
3,374,371 - fliD SO3235 0.38 +1.1
3,374,468 + fliD SO3235 0.45 +1.2
3,374,642 - fliD SO3235 0.58 -2.9
3,374,642 - fliD SO3235 0.58 +0.0
3,374,698 + fliD SO3235 0.62 -0.8
3,374,740 + fliD SO3235 0.65 -0.6

Or see this region's nucleotide sequence