Strain Fitness in Shewanella oneidensis MR-1 around SO3212

Experiment: Fumarate electron acceptor; ground control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfliA and flhG overlap by 8 nucleotidesflhG and flhF are separated by 34 nucleotidesflhF and flhA are separated by 11 nucleotides SO3210: fliA - RNA polymerase sigma-27 factor (NCBI ptt file), at 3,350,155 to 3,350,868 fliA SO3211: flhG - flagellar biosynthetic protein FlhG (NCBI ptt file), at 3,350,861 to 3,351,712 flhG SO3212: flhF - flagellar biosynthetic protein FlhF (NCBI ptt file), at 3,351,747 to 3,353,123 flhF SO3213: flhA - flagellar biosynthesis protein FlhA (NCBI ptt file), at 3,353,135 to 3,355,234 flhA Position (kb) 3351 3352 3353 3354Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3at 3350.845 kb on + strandat 3350.853 kb on - strandat 3350.853 kb on - strandat 3350.853 kb on - strandat 3351.311 kb on - strand, within flhGat 3351.457 kb on - strand, within flhGat 3351.511 kb on - strand, within flhGat 3351.571 kb on - strand, within flhGat 3351.591 kb on + strand, within flhGat 3351.638 kb on + strandat 3351.646 kb on - strandat 3351.770 kb on - strandat 3351.770 kb on - strandat 3351.982 kb on - strand, within flhFat 3352.025 kb on - strand, within flhFat 3352.032 kb on + strand, within flhFat 3352.045 kb on - strand, within flhFat 3352.109 kb on + strand, within flhFat 3352.260 kb on + strand, within flhFat 3352.310 kb on - strand, within flhFat 3352.383 kb on + strand, within flhFat 3352.621 kb on - strand, within flhFat 3352.690 kb on + strand, within flhFat 3352.743 kb on - strand, within flhFat 3352.896 kb on + strand, within flhFat 3353.048 kb on + strandat 3353.056 kb on - strandat 3353.143 kb on - strandat 3353.234 kb on + strandat 3353.262 kb on - strandat 3353.506 kb on + strand, within flhAat 3353.689 kb on - strand, within flhAat 3353.788 kb on + strand, within flhAat 3353.795 kb on + strand, within flhAat 3353.906 kb on - strand, within flhAat 3353.986 kb on + strand, within flhAat 3354.057 kb on + strand, within flhAat 3354.057 kb on + strand, within flhAat 3354.065 kb on - strand, within flhA

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Per-strain Table

Position Strand Gene LocusTag Fraction Fumarate electron acceptor; ground control
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3,350,845 + -4.8
3,350,853 - -0.4
3,350,853 - -2.3
3,350,853 - -2.8
3,351,311 - flhG SO3211 0.53 -1.4
3,351,457 - flhG SO3211 0.70 -1.6
3,351,511 - flhG SO3211 0.76 -1.6
3,351,571 - flhG SO3211 0.83 -1.1
3,351,591 + flhG SO3211 0.86 -1.1
3,351,638 + +1.2
3,351,646 - +0.2
3,351,770 - -0.1
3,351,770 - -2.0
3,351,982 - flhF SO3212 0.17 +2.9
3,352,025 - flhF SO3212 0.20 -3.8
3,352,032 + flhF SO3212 0.21 -1.1
3,352,045 - flhF SO3212 0.22 -1.7
3,352,109 + flhF SO3212 0.26 -0.8
3,352,260 + flhF SO3212 0.37 -1.1
3,352,310 - flhF SO3212 0.41 -2.7
3,352,383 + flhF SO3212 0.46 +1.2
3,352,621 - flhF SO3212 0.63 -2.7
3,352,690 + flhF SO3212 0.68 +0.2
3,352,743 - flhF SO3212 0.72 -1.1
3,352,896 + flhF SO3212 0.83 -1.6
3,353,048 + -0.4
3,353,056 - -0.1
3,353,143 - -1.7
3,353,234 + -2.0
3,353,262 - -3.4
3,353,506 + flhA SO3213 0.18 +1.5
3,353,689 - flhA SO3213 0.26 +0.9
3,353,788 + flhA SO3213 0.31 +0.6
3,353,795 + flhA SO3213 0.31 -2.4
3,353,906 - flhA SO3213 0.37 -1.5
3,353,986 + flhA SO3213 0.41 +0.1
3,354,057 + flhA SO3213 0.44 -1.9
3,354,057 + flhA SO3213 0.44 +0.4
3,354,065 - flhA SO3213 0.44 +1.1

Or see this region's nucleotide sequence