Strain Fitness in Shewanella oneidensis MR-1 around SO0173

Experiment: Fumarate electron acceptor; ground control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgspI and gspJ overlap by 20 nucleotidesgspJ and gspK overlap by 4 nucleotidesgspK and gspL are separated by 48 nucleotides SO0171: gspI - general secretion pathway protein I (NCBI ptt file), at 178,274 to 178,642 gspI SO0172: gspJ - general secretion pathway protein J (NCBI ptt file), at 178,623 to 179,390 gspJ SO0173: gspK - general secretion pathway protein K (NCBI ptt file), at 179,387 to 180,385 gspK SO0174: gspL - general secretion pathway protein L (NCBI ptt file), at 180,434 to 181,621 gspL Position (kb) 179 180 181Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 178.440 kb on + strand, within gspIat 178.516 kb on - strand, within gspIat 178.605 kb on - strand, within gspIat 178.654 kb on + strandat 178.654 kb on + strandat 178.654 kb on + strandat 178.654 kb on + strandat 178.654 kb on + strandat 178.654 kb on + strandat 178.662 kb on - strandat 178.662 kb on - strandat 178.700 kb on + strand, within gspJat 178.730 kb on - strand, within gspJat 178.742 kb on - strand, within gspJat 178.744 kb on + strand, within gspJat 178.816 kb on + strand, within gspJat 178.824 kb on - strand, within gspJat 178.849 kb on - strand, within gspJat 178.850 kb on + strand, within gspJat 178.867 kb on + strand, within gspJat 178.943 kb on + strand, within gspJat 178.943 kb on + strand, within gspJat 178.950 kb on - strand, within gspJat 178.951 kb on - strand, within gspJat 179.018 kb on - strand, within gspJat 179.151 kb on + strand, within gspJat 179.157 kb on + strand, within gspJat 179.220 kb on - strand, within gspJat 179.273 kb on + strand, within gspJat 179.303 kb on - strand, within gspJat 179.394 kb on + strandat 179.436 kb on - strandat 179.544 kb on - strand, within gspKat 179.635 kb on + strand, within gspKat 179.645 kb on + strand, within gspKat 179.785 kb on + strand, within gspKat 179.849 kb on - strand, within gspKat 179.892 kb on + strand, within gspKat 180.055 kb on + strand, within gspKat 180.102 kb on - strand, within gspKat 180.209 kb on - strand, within gspKat 180.410 kb on + strandat 180.418 kb on - strandat 180.424 kb on + strandat 180.476 kb on - strandat 180.493 kb on + strandat 180.538 kb on + strandat 180.546 kb on - strandat 180.546 kb on - strandat 180.569 kb on - strand, within gspLat 180.569 kb on - strand, within gspLat 180.569 kb on - strand, within gspLat 180.628 kb on - strand, within gspLat 180.628 kb on - strand, within gspLat 180.697 kb on - strand, within gspLat 180.910 kb on - strand, within gspLat 180.920 kb on + strand, within gspLat 180.932 kb on + strand, within gspLat 181.035 kb on + strand, within gspLat 181.078 kb on - strand, within gspLat 181.147 kb on - strand, within gspLat 181.225 kb on - strand, within gspLat 181.281 kb on - strand, within gspLat 181.311 kb on - strand, within gspLat 181.364 kb on + strand, within gspLat 181.364 kb on + strand, within gspLat 181.372 kb on - strand, within gspLat 181.372 kb on - strand, within gspL

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Per-strain Table

Position Strand Gene LocusTag Fraction Fumarate electron acceptor; ground control
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178,440 + gspI SO0171 0.45 -1.1
178,516 - gspI SO0171 0.66 -0.3
178,605 - gspI SO0171 0.90 -0.6
178,654 + -1.1
178,654 + -0.3
178,654 + -3.2
178,654 + -1.3
178,654 + -0.6
178,654 + -2.2
178,662 - -2.1
178,662 - -1.3
178,700 + gspJ SO0172 0.10 +1.7
178,730 - gspJ SO0172 0.14 -0.4
178,742 - gspJ SO0172 0.15 -2.1
178,744 + gspJ SO0172 0.16 +0.7
178,816 + gspJ SO0172 0.25 -1.3
178,824 - gspJ SO0172 0.26 -1.4
178,849 - gspJ SO0172 0.29 -1.9
178,850 + gspJ SO0172 0.30 -1.7
178,867 + gspJ SO0172 0.32 +0.7
178,943 + gspJ SO0172 0.42 -1.4
178,943 + gspJ SO0172 0.42 -0.6
178,950 - gspJ SO0172 0.43 +0.7
178,951 - gspJ SO0172 0.43 -0.9
179,018 - gspJ SO0172 0.51 -1.6
179,151 + gspJ SO0172 0.69 -2.1
179,157 + gspJ SO0172 0.70 +0.1
179,220 - gspJ SO0172 0.78 -0.9
179,273 + gspJ SO0172 0.85 +1.4
179,303 - gspJ SO0172 0.89 -2.0
179,394 + -2.4
179,436 - -1.6
179,544 - gspK SO0173 0.16 -2.6
179,635 + gspK SO0173 0.25 -2.5
179,645 + gspK SO0173 0.26 -1.1
179,785 + gspK SO0173 0.40 -1.3
179,849 - gspK SO0173 0.46 -1.9
179,892 + gspK SO0173 0.51 -0.9
180,055 + gspK SO0173 0.67 -2.3
180,102 - gspK SO0173 0.72 -2.4
180,209 - gspK SO0173 0.82 -0.6
180,410 + +0.5
180,418 - -0.6
180,424 + +2.4
180,476 - -2.0
180,493 + -1.3
180,538 + -2.3
180,546 - -1.1
180,546 - +0.7
180,569 - gspL SO0174 0.11 +1.7
180,569 - gspL SO0174 0.11 -1.3
180,569 - gspL SO0174 0.11 +0.7
180,628 - gspL SO0174 0.16 -1.7
180,628 - gspL SO0174 0.16 -0.9
180,697 - gspL SO0174 0.22 +0.7
180,910 - gspL SO0174 0.40 -2.2
180,920 + gspL SO0174 0.41 +1.1
180,932 + gspL SO0174 0.42 -1.3
181,035 + gspL SO0174 0.51 +2.6
181,078 - gspL SO0174 0.54 -1.3
181,147 - gspL SO0174 0.60 -1.4
181,225 - gspL SO0174 0.67 -1.1
181,281 - gspL SO0174 0.71 -1.9
181,311 - gspL SO0174 0.74 +0.7
181,364 + gspL SO0174 0.78 -0.6
181,364 + gspL SO0174 0.78 -0.6
181,372 - gspL SO0174 0.79 -0.9
181,372 - gspL SO0174 0.79 +0.1

Or see this region's nucleotide sequence